Oxygen tensions in multicell spheroids of two cell lines.
O2 tensions (Po2) were measured with microelectrodes in multicellular spheroids from EMT6/Ro and V-79-171-B cells. The measurements were performed in spheroids kept in flowing growth medium that was equilibrated with 5% CO2 and air at a temperature of 37 degrees C and contained 5.5 mM glucose. The r...
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| Format: | Online |
| Language: | English |
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1982
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010909/ |
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pubmed-2010909 |
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pubmed-20109092009-09-10 Oxygen tensions in multicell spheroids of two cell lines. Mueller-Klieser, W. F. Sutherland, R. M. Research Article O2 tensions (Po2) were measured with microelectrodes in multicellular spheroids from EMT6/Ro and V-79-171-B cells. The measurements were performed in spheroids kept in flowing growth medium that was equilibrated with 5% CO2 and air at a temperature of 37 degrees C and contained 5.5 mM glucose. The recorded Po2 profiles are characterized by a diffusion-depleted zone surrounding the spheroids and by a steep drop in Po2 within the spheroids over mean distance of 220 and 188 micrometer from the surface of EMT6/Ro and V-79-171B spheroids over mean distance of 220 and 188 micrometer from the surface of EMT6/Ro and V-79-171B spheroids respectively. Smaller spheroid exhibit parabolic Po2 profiles, larger ones show a central plateau. The region of the steep decrease in Po2 corresponds to the thickness of the viable rim: the plateau region is created by the absence of O2 consumption in the central necrotic area. Po2 in the centre of EMT6/Ro spheroids decreased from 66 mmHg at a diameter of 400 micrometer to 13 mmHg at a diameter of 1000 micrometer. Under the present conditions during growth and in the experiments, values below 5 mmHg were recorded only in spheroids 1200 micrometer. Comparably low Po2 was recorded in V-79 spheroids with diameters of 650 micrometer +. In spheroids of this cell type with a diameter of 400 micrometer, Po2 was 42 mmHg. The findings provide evidence that necrosis may arise at average Po2 of 57 and 42 mmHg in EMT6/Ro and V-79-171B spheroids, respectively, grown under the conditions described. 1982-02 /pmc/articles/PMC2010909/ /pubmed/7059474 Text en |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Mueller-Klieser, W. F. Sutherland, R. M. |
| spellingShingle |
Mueller-Klieser, W. F. Sutherland, R. M. Oxygen tensions in multicell spheroids of two cell lines. |
| author_facet |
Mueller-Klieser, W. F. Sutherland, R. M. |
| author_sort |
Mueller-Klieser, W. F. |
| title |
Oxygen tensions in multicell spheroids of two cell lines. |
| title_short |
Oxygen tensions in multicell spheroids of two cell lines. |
| title_full |
Oxygen tensions in multicell spheroids of two cell lines. |
| title_fullStr |
Oxygen tensions in multicell spheroids of two cell lines. |
| title_full_unstemmed |
Oxygen tensions in multicell spheroids of two cell lines. |
| title_sort |
oxygen tensions in multicell spheroids of two cell lines. |
| description |
O2 tensions (Po2) were measured with microelectrodes in multicellular spheroids from EMT6/Ro and V-79-171-B cells. The measurements were performed in spheroids kept in flowing growth medium that was equilibrated with 5% CO2 and air at a temperature of 37 degrees C and contained 5.5 mM glucose. The recorded Po2 profiles are characterized by a diffusion-depleted zone surrounding the spheroids and by a steep drop in Po2 within the spheroids over mean distance of 220 and 188 micrometer from the surface of EMT6/Ro and V-79-171B spheroids over mean distance of 220 and 188 micrometer from the surface of EMT6/Ro and V-79-171B spheroids respectively. Smaller spheroid exhibit parabolic Po2 profiles, larger ones show a central plateau. The region of the steep decrease in Po2 corresponds to the thickness of the viable rim: the plateau region is created by the absence of O2 consumption in the central necrotic area. Po2 in the centre of EMT6/Ro spheroids decreased from 66 mmHg at a diameter of 400 micrometer to 13 mmHg at a diameter of 1000 micrometer. Under the present conditions during growth and in the experiments, values below 5 mmHg were recorded only in spheroids 1200 micrometer. Comparably low Po2 was recorded in V-79 spheroids with diameters of 650 micrometer +. In spheroids of this cell type with a diameter of 400 micrometer, Po2 was 42 mmHg. The findings provide evidence that necrosis may arise at average Po2 of 57 and 42 mmHg in EMT6/Ro and V-79-171B spheroids, respectively, grown under the conditions described. |
| publishDate |
1982 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010909/ |
| _version_ |
1611403838669979648 |