Characterization of gangliosides in human leucocytes.
Lipids extracted from leucocyte pellets with chloroform-methanol were applied to a DEAE-Sephadex column an the gangliosides were eluted with 0.2M sodium acetate in methanol. The eluate was desalted by Sephadex G-50 column chromatography. The purified ganglioside was spotted on the high-performance t...
Main Authors: | , , , , , |
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Format: | Online |
Language: | English |
Published: |
1980
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2010586/ |