Interleukin-1 beta and interleukin-6 release by peripheral blood monocytes in head and neck cancer.
In patients with advanced head and neck squamous cell carcinoma (HNSC), evidence of cell-mediated immunity and monocyte functional abnormalities has been reported. We studied the production of interleukin 1 beta (IL-1 beta) and interleukin 6 (IL-6) by peripheral blood monocytes from 22 patients with...
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| Format: | Online |
| Language: | English |
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1993
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968396/ |
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pubmed-1968396 |
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pubmed-19683962009-09-10 Interleukin-1 beta and interleukin-6 release by peripheral blood monocytes in head and neck cancer. Gallo, O. Gori, A. M. Attanasio, M. Martini, F. Giusti, B. Boddi, M. Gallina, E. Fini, O. Abbate, R. Research Article In patients with advanced head and neck squamous cell carcinoma (HNSC), evidence of cell-mediated immunity and monocyte functional abnormalities has been reported. We studied the production of interleukin 1 beta (IL-1 beta) and interleukin 6 (IL-6) by peripheral blood monocytes from 22 patients with HNSC (12 larynx and ten oral cavity cancers) in comparison with monocyte cytokine production of age-matched healthy subjects. Pure monocytes were incubated with and without lipopolysaccharides (LPS) (10 micrograms ml-1) for 4 h at 37 degrees C and IL-1 beta and IL-6 concentrations were determined in supernatants by specific ELISA. There was no significant difference in IL-1 beta levels in monocyte supernatants from cancer in comparison to control subjects; conversely, a higher IL-6 production by unstimulated and LPS-activated cells from HNSC patients than from controls was found. No relationship was observed between cytokine production and cancer stage. The regression analysis evidenced a significant correlation between IL-1 beta and IL-6 monocyte-release in HNSC patients and in controls, so suggesting a possible autocrine control of IL-6 production by other cytokines. 1993-09 /pmc/articles/PMC1968396/ /pubmed/8353036 Text en |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Gallo, O. Gori, A. M. Attanasio, M. Martini, F. Giusti, B. Boddi, M. Gallina, E. Fini, O. Abbate, R. |
| spellingShingle |
Gallo, O. Gori, A. M. Attanasio, M. Martini, F. Giusti, B. Boddi, M. Gallina, E. Fini, O. Abbate, R. Interleukin-1 beta and interleukin-6 release by peripheral blood monocytes in head and neck cancer. |
| author_facet |
Gallo, O. Gori, A. M. Attanasio, M. Martini, F. Giusti, B. Boddi, M. Gallina, E. Fini, O. Abbate, R. |
| author_sort |
Gallo, O. |
| title |
Interleukin-1 beta and interleukin-6 release by peripheral blood monocytes in head and neck cancer. |
| title_short |
Interleukin-1 beta and interleukin-6 release by peripheral blood monocytes in head and neck cancer. |
| title_full |
Interleukin-1 beta and interleukin-6 release by peripheral blood monocytes in head and neck cancer. |
| title_fullStr |
Interleukin-1 beta and interleukin-6 release by peripheral blood monocytes in head and neck cancer. |
| title_full_unstemmed |
Interleukin-1 beta and interleukin-6 release by peripheral blood monocytes in head and neck cancer. |
| title_sort |
interleukin-1 beta and interleukin-6 release by peripheral blood monocytes in head and neck cancer. |
| description |
In patients with advanced head and neck squamous cell carcinoma (HNSC), evidence of cell-mediated immunity and monocyte functional abnormalities has been reported. We studied the production of interleukin 1 beta (IL-1 beta) and interleukin 6 (IL-6) by peripheral blood monocytes from 22 patients with HNSC (12 larynx and ten oral cavity cancers) in comparison with monocyte cytokine production of age-matched healthy subjects. Pure monocytes were incubated with and without lipopolysaccharides (LPS) (10 micrograms ml-1) for 4 h at 37 degrees C and IL-1 beta and IL-6 concentrations were determined in supernatants by specific ELISA. There was no significant difference in IL-1 beta levels in monocyte supernatants from cancer in comparison to control subjects; conversely, a higher IL-6 production by unstimulated and LPS-activated cells from HNSC patients than from controls was found. No relationship was observed between cytokine production and cancer stage. The regression analysis evidenced a significant correlation between IL-1 beta and IL-6 monocyte-release in HNSC patients and in controls, so suggesting a possible autocrine control of IL-6 production by other cytokines. |
| publishDate |
1993 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968396/ |
| _version_ |
1611399633791090688 |