Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast
Ubiquitin-protein ligases (E3s) are responsible for target recognition and regulate stability, localization or function of their substrates. However, the substrates of most E3 enzymes remain unknown. Here, we describe the development of a novel proteomic in vitro ubiquitination screen using a protei...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
Nature Publishing Group
2007
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1911201/ |