Ultra fast miniaturized real-time PCR: 40 cycles in less than six minutes

We have designed, fabricated and tested a real-time PCR chip capable of conducting one thermal cycle in 8.5 s. This corresponds to 40 cycles of PCR in 5 min and 40 s. The PCR system was made of silicon micromachined into the shape of a cantilever terminated with a disc. The thin film heater and a te...

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Bibliographic Details
Main Authors: Neuzil, Pavel, Zhang, Chunyan, Pipper, Juergen, Oh, Sharon, Zhuo, Lang
Format: Online
Language:English
Published: Oxford University Press 2006
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1904101/