R.KpnI, an HNH superfamily REase, exhibits differential discrimination at non-canonical sequences in the presence of Ca2+ and Mg2+

R.KpnI, an HNH superfamily REase, exhibits differential discrimination at non-canonical sequences in the presence of Ca2+ and Mg2+

KpnI REase recognizes palindromic sequence, GGTAC↓C, and forms complex in the absence of divalent metal ions, but requires the ions for DNA cleavage. Unlike most other REases, R.KpnI shows promiscuous DNA cleavage in the presence of Mg2+. Surprisingly, Ca2+ suppresses the Mg2+-mediated promiscuous a...

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Bibliographic Details
Main Authors: Saravanan, Matheshwaran, Vasu, Kommireddy, Kanakaraj, Radhakrishnan, Rao, Desirazu N., Nagaraja, Valakunja
Format: Online
Language:English
Published: Oxford University Press 2007
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1885652/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1885652/

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