Identification of a Novel Polyomavirus from Patients with Acute Respiratory Tract Infections
We report the identification of a novel polyomavirus present in respiratory secretions from human patients with symptoms of acute respiratory tract infection. The virus was initially detected in a nasopharyngeal aspirate from a 3-year-old child from Australia diagnosed with pneumonia. A random libra...
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| Format: | Online |
| Language: | English |
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Public Library of Science
2007
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1864993/ |
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pubmed-1864993 |
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pubmed-18649932007-05-04 Identification of a Novel Polyomavirus from Patients with Acute Respiratory Tract Infections Gaynor, Anne M Nissen, Michael D Whiley, David M Mackay, Ian M Lambert, Stephen B Wu, Guang Brennan, Daniel C Storch, Gregory A Sloots, Theo P Wang, David Research Article We report the identification of a novel polyomavirus present in respiratory secretions from human patients with symptoms of acute respiratory tract infection. The virus was initially detected in a nasopharyngeal aspirate from a 3-year-old child from Australia diagnosed with pneumonia. A random library was generated from nucleic acids extracted from the nasopharyngeal aspirate and analyzed by high throughput DNA sequencing. Multiple DNA fragments were cloned that possessed limited homology to known polyomaviruses. We subsequently sequenced the entire virus genome of 5,229 bp, henceforth referred to as WU virus, and found it to have genomic features characteristic of the family Polyomaviridae. The genome was predicted to encode small T antigen, large T antigen, and three capsid proteins: VP1, VP2, and VP3. Phylogenetic analysis clearly revealed that the WU virus was divergent from all known polyomaviruses. Screening of 2,135 patients with acute respiratory tract infections in Brisbane, Queensland, Australia, and St. Louis, Missouri, United States, using WU virus–specific PCR primers resulted in the detection of 43 additional specimens that contained WU virus. The presence of multiple instances of the virus in two continents suggests that this virus is geographically widespread in the human population and raises the possibility that the WU virus may be a human pathogen. Public Library of Science 2007-05 2007-05-04 /pmc/articles/PMC1864993/ /pubmed/17480120 http://dx.doi.org/10.1371/journal.ppat.0030064 Text en © 2007 Gaynor et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Gaynor, Anne M Nissen, Michael D Whiley, David M Mackay, Ian M Lambert, Stephen B Wu, Guang Brennan, Daniel C Storch, Gregory A Sloots, Theo P Wang, David |
| spellingShingle |
Gaynor, Anne M Nissen, Michael D Whiley, David M Mackay, Ian M Lambert, Stephen B Wu, Guang Brennan, Daniel C Storch, Gregory A Sloots, Theo P Wang, David Identification of a Novel Polyomavirus from Patients with Acute Respiratory Tract Infections |
| author_facet |
Gaynor, Anne M Nissen, Michael D Whiley, David M Mackay, Ian M Lambert, Stephen B Wu, Guang Brennan, Daniel C Storch, Gregory A Sloots, Theo P Wang, David |
| author_sort |
Gaynor, Anne M |
| title |
Identification of a Novel Polyomavirus from Patients with Acute Respiratory Tract Infections |
| title_short |
Identification of a Novel Polyomavirus from Patients with Acute Respiratory Tract Infections |
| title_full |
Identification of a Novel Polyomavirus from Patients with Acute Respiratory Tract Infections |
| title_fullStr |
Identification of a Novel Polyomavirus from Patients with Acute Respiratory Tract Infections |
| title_full_unstemmed |
Identification of a Novel Polyomavirus from Patients with Acute Respiratory Tract Infections |
| title_sort |
identification of a novel polyomavirus from patients with acute respiratory tract infections |
| description |
We report the identification of a novel polyomavirus present in respiratory secretions from human patients with symptoms of acute respiratory tract infection. The virus was initially detected in a nasopharyngeal aspirate from a 3-year-old child from Australia diagnosed with pneumonia. A random library was generated from nucleic acids extracted from the nasopharyngeal aspirate and analyzed by high throughput DNA sequencing. Multiple DNA fragments were cloned that possessed limited homology to known polyomaviruses. We subsequently sequenced the entire virus genome of 5,229 bp, henceforth referred to as WU virus, and found it to have genomic features characteristic of the family Polyomaviridae. The genome was predicted to encode small T antigen, large T antigen, and three capsid proteins: VP1, VP2, and VP3. Phylogenetic analysis clearly revealed that the WU virus was divergent from all known polyomaviruses. Screening of 2,135 patients with acute respiratory tract infections in Brisbane, Queensland, Australia, and St. Louis, Missouri, United States, using WU virus–specific PCR primers resulted in the detection of 43 additional specimens that contained WU virus. The presence of multiple instances of the virus in two continents suggests that this virus is geographically widespread in the human population and raises the possibility that the WU virus may be a human pathogen. |
| publisher |
Public Library of Science |
| publishDate |
2007 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1864993/ |
| _version_ |
1611396066357280768 |