Quantitative trait loci and candidate gene mapping of aluminum tolerance in diploid alfalfa

Aluminum (Al) toxicity in acid soils is a major limitation to the production of alfalfa (Medicago sativa subsp. sativa L.) in the USA. Developing Al-tolerant alfalfa cultivars is one approach to overcome this constraint. Accessions of wild diploid alfalfa (M. sativa subsp. coerulea) have been found...

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Main Authors:	Narasimhamoorthy, B., Bouton, J. H., Olsen, K. M., Sledge, M. K.
Format:	Online
Language:	English
Published:	Springer-Verlag 2007
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1805042/

id	pubmed-1805042
recordtype	oai_dc
spelling	pubmed-18050422007-02-26 Quantitative trait loci and candidate gene mapping of aluminum tolerance in diploid alfalfa Narasimhamoorthy, B. Bouton, J. H. Olsen, K. M. Sledge, M. K. Original Paper Aluminum (Al) toxicity in acid soils is a major limitation to the production of alfalfa (Medicago sativa subsp. sativa L.) in the USA. Developing Al-tolerant alfalfa cultivars is one approach to overcome this constraint. Accessions of wild diploid alfalfa (M. sativa subsp. coerulea) have been found to be a source of useful genes for Al tolerance. Previously, two genomic regions associated with Al tolerance were identified in this diploid species using restriction fragment length polymorphism (RFLP) markers and single marker analysis. This study was conducted to identify additional Al-tolerance quantitative trait loci (QTLs); to identify simple sequence repeat (SSR) markers that flank the previously identified QTLs; to map candidate genes associated with Al tolerance from other plant species; and to test for co-localization with mapped QTLs. A genetic linkage map was constructed using EST-SSR markers in a population of 130 BC1F1 plants derived from the cross between Al-sensitive and Al-tolerant genotypes. Three putative QTLs on linkage groups LG I, LG II and LG III, explaining 38, 16 and 27% of the phenotypic variation, respectively, were identified. Six candidate gene markers designed from Medicago truncatula ESTs that showed homology to known Al-tolerance genes identified in other plant species were placed on the QTL map. A marker designed from a candidate gene involved in malic acid release mapped near a marginally significant QTL (LOD 2.83) on LG I. The SSR markers flanking these QTLs will be useful for transferring them to cultivated alfalfa via marker-assisted selection and for pyramiding Al tolerance QTLs. Springer-Verlag 2007-01-12 2007-03 /pmc/articles/PMC1805042/ /pubmed/17219204 http://dx.doi.org/10.1007/s00122-006-0488-7 Text en © Springer-Verlag 2007
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Narasimhamoorthy, B. Bouton, J. H. Olsen, K. M. Sledge, M. K.
spellingShingle	Narasimhamoorthy, B. Bouton, J. H. Olsen, K. M. Sledge, M. K. Quantitative trait loci and candidate gene mapping of aluminum tolerance in diploid alfalfa
author_facet	Narasimhamoorthy, B. Bouton, J. H. Olsen, K. M. Sledge, M. K.
author_sort	Narasimhamoorthy, B.
title	Quantitative trait loci and candidate gene mapping of aluminum tolerance in diploid alfalfa
title_short	Quantitative trait loci and candidate gene mapping of aluminum tolerance in diploid alfalfa
title_full	Quantitative trait loci and candidate gene mapping of aluminum tolerance in diploid alfalfa
title_fullStr	Quantitative trait loci and candidate gene mapping of aluminum tolerance in diploid alfalfa
title_full_unstemmed	Quantitative trait loci and candidate gene mapping of aluminum tolerance in diploid alfalfa
title_sort	quantitative trait loci and candidate gene mapping of aluminum tolerance in diploid alfalfa
description	Aluminum (Al) toxicity in acid soils is a major limitation to the production of alfalfa (Medicago sativa subsp. sativa L.) in the USA. Developing Al-tolerant alfalfa cultivars is one approach to overcome this constraint. Accessions of wild diploid alfalfa (M. sativa subsp. coerulea) have been found to be a source of useful genes for Al tolerance. Previously, two genomic regions associated with Al tolerance were identified in this diploid species using restriction fragment length polymorphism (RFLP) markers and single marker analysis. This study was conducted to identify additional Al-tolerance quantitative trait loci (QTLs); to identify simple sequence repeat (SSR) markers that flank the previously identified QTLs; to map candidate genes associated with Al tolerance from other plant species; and to test for co-localization with mapped QTLs. A genetic linkage map was constructed using EST-SSR markers in a population of 130 BC1F1 plants derived from the cross between Al-sensitive and Al-tolerant genotypes. Three putative QTLs on linkage groups LG I, LG II and LG III, explaining 38, 16 and 27% of the phenotypic variation, respectively, were identified. Six candidate gene markers designed from Medicago truncatula ESTs that showed homology to known Al-tolerance genes identified in other plant species were placed on the QTL map. A marker designed from a candidate gene involved in malic acid release mapped near a marginally significant QTL (LOD 2.83) on LG I. The SSR markers flanking these QTLs will be useful for transferring them to cultivated alfalfa via marker-assisted selection and for pyramiding Al tolerance QTLs.
publisher	Springer-Verlag
publishDate	2007
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1805042/
_version_	1611394575922888704

Quantitative trait loci and candidate gene mapping of aluminum tolerance in diploid alfalfa

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