A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes

In order to facilitate the functional analysis of rice genes, we produced about 50,000 insertion lines with the endogenous retrotransposon Tos17. Phenotypes of these lines in the M2 generation were observed in the field and characterized based on 53 phenotype descriptors. Nearly half of the lines sh...

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Main Authors: Miyao, Akio, Iwasaki, Yukimoto, Kitano, Hidemi, Itoh, Jun-Ichi, Maekawa, Masahiko, Murata, Kazumasa, Yatou, Osamu, Nagato, Yasuo, Hirochika, Hirohiko
Format: Online
Language:English
Published: Kluwer Academic Publishers 2006
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1805041/
id pubmed-1805041
recordtype oai_dc
spelling pubmed-18050412007-02-26 A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes Miyao, Akio Iwasaki, Yukimoto Kitano, Hidemi Itoh, Jun-Ichi Maekawa, Masahiko Murata, Kazumasa Yatou, Osamu Nagato, Yasuo Hirochika, Hirohiko Article In order to facilitate the functional analysis of rice genes, we produced about 50,000 insertion lines with the endogenous retrotransposon Tos17. Phenotypes of these lines in the M2 generation were observed in the field and characterized based on 53 phenotype descriptors. Nearly half of the lines showed more than one mutant phenotype. The most frequently observed phenotype was low fertility, followed by dwarfism. Phenotype data with photographs of each line are stored in the Tos17 mutant panel web-based database with a dataset of sequences flanking Tos17 insertion points in the rice genome (http://tos.nias.affrc.go.jp/). This combination of phenotypic and flanking sequence data will stimulate the functional analysis of rice genes. Kluwer Academic Publishers 2006-12-19 2007-03 /pmc/articles/PMC1805041/ /pubmed/17180734 http://dx.doi.org/10.1007/s11103-006-9118-7 Text en © Springer Science+Business Media B.V. 2006
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Miyao, Akio
Iwasaki, Yukimoto
Kitano, Hidemi
Itoh, Jun-Ichi
Maekawa, Masahiko
Murata, Kazumasa
Yatou, Osamu
Nagato, Yasuo
Hirochika, Hirohiko
spellingShingle Miyao, Akio
Iwasaki, Yukimoto
Kitano, Hidemi
Itoh, Jun-Ichi
Maekawa, Masahiko
Murata, Kazumasa
Yatou, Osamu
Nagato, Yasuo
Hirochika, Hirohiko
A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes
author_facet Miyao, Akio
Iwasaki, Yukimoto
Kitano, Hidemi
Itoh, Jun-Ichi
Maekawa, Masahiko
Murata, Kazumasa
Yatou, Osamu
Nagato, Yasuo
Hirochika, Hirohiko
author_sort Miyao, Akio
title A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes
title_short A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes
title_full A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes
title_fullStr A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes
title_full_unstemmed A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes
title_sort large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes
description In order to facilitate the functional analysis of rice genes, we produced about 50,000 insertion lines with the endogenous retrotransposon Tos17. Phenotypes of these lines in the M2 generation were observed in the field and characterized based on 53 phenotype descriptors. Nearly half of the lines showed more than one mutant phenotype. The most frequently observed phenotype was low fertility, followed by dwarfism. Phenotype data with photographs of each line are stored in the Tos17 mutant panel web-based database with a dataset of sequences flanking Tos17 insertion points in the rice genome (http://tos.nias.affrc.go.jp/). This combination of phenotypic and flanking sequence data will stimulate the functional analysis of rice genes.
publisher Kluwer Academic Publishers
publishDate 2006
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1805041/
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