A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes
In order to facilitate the functional analysis of rice genes, we produced about 50,000 insertion lines with the endogenous retrotransposon Tos17. Phenotypes of these lines in the M2 generation were observed in the field and characterized based on 53 phenotype descriptors. Nearly half of the lines sh...
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Kluwer Academic Publishers
2006
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1805041/ |
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pubmed-18050412007-02-26 A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes Miyao, Akio Iwasaki, Yukimoto Kitano, Hidemi Itoh, Jun-Ichi Maekawa, Masahiko Murata, Kazumasa Yatou, Osamu Nagato, Yasuo Hirochika, Hirohiko Article In order to facilitate the functional analysis of rice genes, we produced about 50,000 insertion lines with the endogenous retrotransposon Tos17. Phenotypes of these lines in the M2 generation were observed in the field and characterized based on 53 phenotype descriptors. Nearly half of the lines showed more than one mutant phenotype. The most frequently observed phenotype was low fertility, followed by dwarfism. Phenotype data with photographs of each line are stored in the Tos17 mutant panel web-based database with a dataset of sequences flanking Tos17 insertion points in the rice genome (http://tos.nias.affrc.go.jp/). This combination of phenotypic and flanking sequence data will stimulate the functional analysis of rice genes. Kluwer Academic Publishers 2006-12-19 2007-03 /pmc/articles/PMC1805041/ /pubmed/17180734 http://dx.doi.org/10.1007/s11103-006-9118-7 Text en © Springer Science+Business Media B.V. 2006 |
repository_type |
Open Access Journal |
institution_category |
Foreign Institution |
institution |
US National Center for Biotechnology Information |
building |
NCBI PubMed |
collection |
Online Access |
language |
English |
format |
Online |
author |
Miyao, Akio Iwasaki, Yukimoto Kitano, Hidemi Itoh, Jun-Ichi Maekawa, Masahiko Murata, Kazumasa Yatou, Osamu Nagato, Yasuo Hirochika, Hirohiko |
spellingShingle |
Miyao, Akio Iwasaki, Yukimoto Kitano, Hidemi Itoh, Jun-Ichi Maekawa, Masahiko Murata, Kazumasa Yatou, Osamu Nagato, Yasuo Hirochika, Hirohiko A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes |
author_facet |
Miyao, Akio Iwasaki, Yukimoto Kitano, Hidemi Itoh, Jun-Ichi Maekawa, Masahiko Murata, Kazumasa Yatou, Osamu Nagato, Yasuo Hirochika, Hirohiko |
author_sort |
Miyao, Akio |
title |
A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes |
title_short |
A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes |
title_full |
A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes |
title_fullStr |
A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes |
title_full_unstemmed |
A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes |
title_sort |
large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes |
description |
In order to facilitate the functional analysis of rice genes, we produced about 50,000 insertion lines with the endogenous retrotransposon Tos17. Phenotypes of these lines in the M2 generation were observed in the field and characterized based on 53 phenotype descriptors. Nearly half of the lines showed more than one mutant phenotype. The most frequently observed phenotype was low fertility, followed by dwarfism. Phenotype data with photographs of each line are stored in the Tos17 mutant panel web-based database with a dataset of sequences flanking Tos17 insertion points in the rice genome (http://tos.nias.affrc.go.jp/). This combination of phenotypic and flanking sequence data will stimulate the functional analysis of rice genes. |
publisher |
Kluwer Academic Publishers |
publishDate |
2006 |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1805041/ |
_version_ |
1611394575350366208 |