Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells

Preeclampsia is often accompanied by hypoxia of the placenta and this condition induces apoptosis in trophoblastic cells. The aim of this study was to characterize global changes of apoptosis-related proteins induced by hypoxia in trophoblastic cells so as to clarify the mechanism of hypoxia-induced...

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Main Authors:	Ishioka, Shin-ichi, Ezaka, Yoshiaki, Umemura, Kota, Hayashi, Takuhiro, Endo, Toshiaki, Saito, Tsuyoshi
Format:	Online
Language:	English
Published:	Ivyspring International Publisher 2006
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1796955/

id	pubmed-1796955
recordtype	oai_dc
spelling	pubmed-17969552007-02-13 Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells Ishioka, Shin-ichi Ezaka, Yoshiaki Umemura, Kota Hayashi, Takuhiro Endo, Toshiaki Saito, Tsuyoshi Research Paper Preeclampsia is often accompanied by hypoxia of the placenta and this condition induces apoptosis in trophoblastic cells. The aim of this study was to characterize global changes of apoptosis-related proteins induced by hypoxia in trophoblastic cells so as to clarify the mechanism of hypoxia-induced apoptosis by using the PoweBlot, an antibody-based Western array. Human choriocarcinoma cell line JAR was cultured for 24 hours under aerobic and hypoxic conditions. Hypoxia induced apoptosis accompanied by increased expression of Bcl-x, Caspase-3 and -9, Hsp70, PTEN, and Bag-1. Bad, pan-JNK/SAPK-1, Bcl-2, Bid, and Caspase-8 showed decreased expression. Hypoxia-induced apoptosis was increased with the transfection of a bag-1 antisense oligonucleotide. The bag-1 antisense oligonucleotide affected the expression of Bid, Bad, Bcl-2, JNK, and phosphorylated JNK, although expression of PTEN and Bcl-X did not change. Bag-1 may inhibit apoptosis by suppressing the expression of Bid and Bad. It may also enhance apoptosis by inhibiting the expression of Bcl-2 and by modulating phosphorylation of JNK. Both mitochondrial and stress-activated apoptosis pathways played important roles in the hypoxia induced cell death of trophoblastic cells. These findings will contribute to establish new approach to detect hypoxic stress of the placenta, which leads to preeclampsia and other hypoxia-related obstetrics complications. Ivyspring International Publisher 2006-12-29 /pmc/articles/PMC1796955/ /pubmed/17299580 Text en © Ivyspring International Publisher. This is an open access article. Reproduction is permitted for personal, noncommerical use, provided that the article is in whole, unmodified, and properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Ishioka, Shin-ichi Ezaka, Yoshiaki Umemura, Kota Hayashi, Takuhiro Endo, Toshiaki Saito, Tsuyoshi
spellingShingle	Ishioka, Shin-ichi Ezaka, Yoshiaki Umemura, Kota Hayashi, Takuhiro Endo, Toshiaki Saito, Tsuyoshi Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells
author_facet	Ishioka, Shin-ichi Ezaka, Yoshiaki Umemura, Kota Hayashi, Takuhiro Endo, Toshiaki Saito, Tsuyoshi
author_sort	Ishioka, Shin-ichi
title	Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells
title_short	Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells
title_full	Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells
title_fullStr	Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells
title_full_unstemmed	Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells
title_sort	proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells
description	Preeclampsia is often accompanied by hypoxia of the placenta and this condition induces apoptosis in trophoblastic cells. The aim of this study was to characterize global changes of apoptosis-related proteins induced by hypoxia in trophoblastic cells so as to clarify the mechanism of hypoxia-induced apoptosis by using the PoweBlot, an antibody-based Western array. Human choriocarcinoma cell line JAR was cultured for 24 hours under aerobic and hypoxic conditions. Hypoxia induced apoptosis accompanied by increased expression of Bcl-x, Caspase-3 and -9, Hsp70, PTEN, and Bag-1. Bad, pan-JNK/SAPK-1, Bcl-2, Bid, and Caspase-8 showed decreased expression. Hypoxia-induced apoptosis was increased with the transfection of a bag-1 antisense oligonucleotide. The bag-1 antisense oligonucleotide affected the expression of Bid, Bad, Bcl-2, JNK, and phosphorylated JNK, although expression of PTEN and Bcl-X did not change. Bag-1 may inhibit apoptosis by suppressing the expression of Bid and Bad. It may also enhance apoptosis by inhibiting the expression of Bcl-2 and by modulating phosphorylation of JNK. Both mitochondrial and stress-activated apoptosis pathways played important roles in the hypoxia induced cell death of trophoblastic cells. These findings will contribute to establish new approach to detect hypoxic stress of the placenta, which leads to preeclampsia and other hypoxia-related obstetrics complications.
publisher	Ivyspring International Publisher
publishDate	2006
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1796955/
_version_	1611394236561752064

Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells

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