Biogenesis and Dynamics of Mitochondria during the Cell Cycle: Significance of 3′UTRs
Nowadays, we are facing a renaissance of mitochondria in cancer biology. However, our knowledge of the basic cell biology and on the timing and mechanisms that control the biosynthesis of mitochondrial constituents during progression through the cell cycle of mammalian cells remain largely unknown....
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Public Library of Science
2006
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1762426/ |
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pubmed-17624262007-01-04 Biogenesis and Dynamics of Mitochondria during the Cell Cycle: Significance of 3′UTRs Martínez-Diez, Marta Santamaría, Gema Ortega, Álvaro D. Cuezva, José M. Research Article Nowadays, we are facing a renaissance of mitochondria in cancer biology. However, our knowledge of the basic cell biology and on the timing and mechanisms that control the biosynthesis of mitochondrial constituents during progression through the cell cycle of mammalian cells remain largely unknown. Herein, we document the in vivo changes on mitochondrial morphology and dynamics that accompany cellular mitosis, and illustrate the following key points of the biogenesis of mitochondria during progression of liver cells through the cycle: (i) the replication of nuclear and mitochondrial genomes is synchronized during cellular proliferation, (ii) the accretion of OXPHOS proteins is asynchronously regulated during proliferation being the synthesis of β-F1-ATPase and Hsp60 carried out also at G2/M and, (iii) the biosynthesis of cardiolipin is achieved during the S phase, although full development of the mitochondrial membrane potential (ΔΨm) is attained at G2/M. Furthermore, we demonstrate using reporter constructs that the mechanism regulating the accretion of β-F1-ATPase during cellular proliferation is controlled at the level of mRNA translation by the 3′UTR of the transcript. The 3′UTR-driven synthesis of the protein at G2/M is essential for conferring to the daughter cells the original phenotype of the parental cell. Our findings suggest that alterations on this process may promote deregulated β-F1-ATPase expression in human cancer. Public Library of Science 2006-12-20 /pmc/articles/PMC1762426/ /pubmed/17205111 http://dx.doi.org/10.1371/journal.pone.0000107 Text en Martínez-Diez et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Martínez-Diez, Marta Santamaría, Gema Ortega, Álvaro D. Cuezva, José M. |
| spellingShingle |
Martínez-Diez, Marta Santamaría, Gema Ortega, Álvaro D. Cuezva, José M. Biogenesis and Dynamics of Mitochondria during the Cell Cycle: Significance of 3′UTRs |
| author_facet |
Martínez-Diez, Marta Santamaría, Gema Ortega, Álvaro D. Cuezva, José M. |
| author_sort |
Martínez-Diez, Marta |
| title |
Biogenesis and Dynamics of Mitochondria during the Cell Cycle: Significance of 3′UTRs |
| title_short |
Biogenesis and Dynamics of Mitochondria during the Cell Cycle: Significance of 3′UTRs |
| title_full |
Biogenesis and Dynamics of Mitochondria during the Cell Cycle: Significance of 3′UTRs |
| title_fullStr |
Biogenesis and Dynamics of Mitochondria during the Cell Cycle: Significance of 3′UTRs |
| title_full_unstemmed |
Biogenesis and Dynamics of Mitochondria during the Cell Cycle: Significance of 3′UTRs |
| title_sort |
biogenesis and dynamics of mitochondria during the cell cycle: significance of 3′utrs |
| description |
Nowadays, we are facing a renaissance of mitochondria in cancer biology. However, our knowledge of the basic cell biology and on the timing and mechanisms that control the biosynthesis of mitochondrial constituents during progression through the cell cycle of mammalian cells remain largely unknown. Herein, we document the in vivo changes on mitochondrial morphology and dynamics that accompany cellular mitosis, and illustrate the following key points of the biogenesis of mitochondria during progression of liver cells through the cycle: (i) the replication of nuclear and mitochondrial genomes is synchronized during cellular proliferation, (ii) the accretion of OXPHOS proteins is asynchronously regulated during proliferation being the synthesis of β-F1-ATPase and Hsp60 carried out also at G2/M and, (iii) the biosynthesis of cardiolipin is achieved during the S phase, although full development of the mitochondrial membrane potential (ΔΨm) is attained at G2/M. Furthermore, we demonstrate using reporter constructs that the mechanism regulating the accretion of β-F1-ATPase during cellular proliferation is controlled at the level of mRNA translation by the 3′UTR of the transcript. The 3′UTR-driven synthesis of the protein at G2/M is essential for conferring to the daughter cells the original phenotype of the parental cell. Our findings suggest that alterations on this process may promote deregulated β-F1-ATPase expression in human cancer. |
| publisher |
Public Library of Science |
| publishDate |
2006 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1762426/ |
| _version_ |
1611392762797621248 |