Three-dimensional Numerical Modeling and Computational Fluid Dynamics Simulations to Analyze and Improve Oxygen Availability in the AMC Bioartificial Liver
A numerical model to investigate fluid flow and oxygen (O2) transport and consumption in the AMC-Bioartificial Liver (AMC-BAL) was developed and applied to two representative micro models of the AMC-BAL with two different gas capillary patterns, each combined with two proposed hepatocyte distributio...
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| Format: | Online |
| Language: | English |
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Kluwer Academic Publishers-Plenum Publishers
2006
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1705524/ |
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pubmed-1705524 |
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pubmed-17055242006-12-18 Three-dimensional Numerical Modeling and Computational Fluid Dynamics Simulations to Analyze and Improve Oxygen Availability in the AMC Bioartificial Liver Mareels, Guy Poyck, Paul P. C. Eloot, Sunny Chamuleau, Robert A. F. M. Verdonck, Pascal R. Article A numerical model to investigate fluid flow and oxygen (O2) transport and consumption in the AMC-Bioartificial Liver (AMC-BAL) was developed and applied to two representative micro models of the AMC-BAL with two different gas capillary patterns, each combined with two proposed hepatocyte distributions. Parameter studies were performed on each configuration to gain insight in fluid flow, shear stress distribution and oxygen availability in the AMC-BAL. We assessed the function of the internal oxygenator, the effect of changes in hepatocyte oxygen consumption parameters in time and the effect of the change from an experimental to a clinical setting. In addition, different methodologies were studied to improve cellular oxygen availability, i.e. external oxygenation of culture medium, culture medium flow rate, culture gas oxygen content (pO2) and the number of oxygenation capillaries. Standard operating conditions did not adequately provide all hepatocytes in the AMC-BAL with sufficient oxygen to maintain O2 consumption at minimally 90% of maximal uptake rate. Cellular oxygen availability was optimized by increasing the number of gas capillaries and pO2 of the oxygenation gas by a factor two. Pressure drop over the AMC-BAL and maximal shear stresses were low and not considered to be harmful. This information can be used to increase cellular efficiency and may ultimately lead to a more productive AMC-BAL. Kluwer Academic Publishers-Plenum Publishers 2006-10-10 2006-11 /pmc/articles/PMC1705524/ /pubmed/17031599 http://dx.doi.org/10.1007/s10439-006-9169-6 Text en © Springer Science+Business Media, Inc. 2006 |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Mareels, Guy Poyck, Paul P. C. Eloot, Sunny Chamuleau, Robert A. F. M. Verdonck, Pascal R. |
| spellingShingle |
Mareels, Guy Poyck, Paul P. C. Eloot, Sunny Chamuleau, Robert A. F. M. Verdonck, Pascal R. Three-dimensional Numerical Modeling and Computational Fluid Dynamics Simulations to Analyze and Improve Oxygen Availability in the AMC Bioartificial Liver |
| author_facet |
Mareels, Guy Poyck, Paul P. C. Eloot, Sunny Chamuleau, Robert A. F. M. Verdonck, Pascal R. |
| author_sort |
Mareels, Guy |
| title |
Three-dimensional Numerical Modeling and Computational Fluid Dynamics Simulations to Analyze and Improve Oxygen Availability in the AMC Bioartificial Liver |
| title_short |
Three-dimensional Numerical Modeling and Computational Fluid Dynamics Simulations to Analyze and Improve Oxygen Availability in the AMC Bioartificial Liver |
| title_full |
Three-dimensional Numerical Modeling and Computational Fluid Dynamics Simulations to Analyze and Improve Oxygen Availability in the AMC Bioartificial Liver |
| title_fullStr |
Three-dimensional Numerical Modeling and Computational Fluid Dynamics Simulations to Analyze and Improve Oxygen Availability in the AMC Bioartificial Liver |
| title_full_unstemmed |
Three-dimensional Numerical Modeling and Computational Fluid Dynamics Simulations to Analyze and Improve Oxygen Availability in the AMC Bioartificial Liver |
| title_sort |
three-dimensional numerical modeling and computational fluid dynamics simulations to analyze and improve oxygen availability in the amc bioartificial liver |
| description |
A numerical model to investigate fluid flow and oxygen (O2) transport and consumption in the AMC-Bioartificial Liver (AMC-BAL) was developed and applied to two representative micro models of the AMC-BAL with two different gas capillary patterns, each combined with two proposed hepatocyte distributions. Parameter studies were performed on each configuration to gain insight in fluid flow, shear stress distribution and oxygen availability in the AMC-BAL. We assessed the function of the internal oxygenator, the effect of changes in hepatocyte oxygen consumption parameters in time and the effect of the change from an experimental to a clinical setting. In addition, different methodologies were studied to improve cellular oxygen availability, i.e. external oxygenation of culture medium, culture medium flow rate, culture gas oxygen content (pO2) and the number of oxygenation capillaries. Standard operating conditions did not adequately provide all hepatocytes in the AMC-BAL with sufficient oxygen to maintain O2 consumption at minimally 90% of maximal uptake rate. Cellular oxygen availability was optimized by increasing the number of gas capillaries and pO2 of the oxygenation gas by a factor two. Pressure drop over the AMC-BAL and maximal shear stresses were low and not considered to be harmful. This information can be used to increase cellular efficiency and may ultimately lead to a more productive AMC-BAL. |
| publisher |
Kluwer Academic Publishers-Plenum Publishers |
| publishDate |
2006 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1705524/ |
| _version_ |
1611392091820130304 |