Podophyllum hexandrum Offers Radioprotection by Modulating Free Radical Flux: Role of Aryl-Tetralin Lignans

We have evaluated the effect of variation in aryl-tetralin lignans on the radioprotective properties of Podophyllum hexandrum. Two fractionated fractions of P. hexandrum [methanolic (S1) and chloroform fractions (S2)], with varying aryl-tetralin lignan content were utilized for the present study. Th...

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Main Authors:	Chawla, Raman, Arora, Rajesh, Singh, Shikha, Sagar, R.K., Sharma, Rakesh Kumar, Kumar, R., Sharma, A., Tripathi, R. P., Puri, S. C., Khan, H. A., Shawl, A. S., Sultan, P., Krishan, Tej, Qazi, G. N.
Format:	Online
Language:	English
Published:	Oxford University Press 2006
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1697744/

id	pubmed-1697744
recordtype	oai_dc
spelling	pubmed-16977442006-12-14 Podophyllum hexandrum Offers Radioprotection by Modulating Free Radical Flux: Role of Aryl-Tetralin Lignans Chawla, Raman Arora, Rajesh Singh, Shikha Sagar, R.K. Sharma, Rakesh Kumar Kumar, R. Sharma, A. Tripathi, R. P. Puri, S. C. Khan, H. A. Shawl, A. S. Sultan, P. Krishan, Tej Qazi, G. N. Original Articles We have evaluated the effect of variation in aryl-tetralin lignans on the radioprotective properties of Podophyllum hexandrum. Two fractionated fractions of P. hexandrum [methanolic (S1) and chloroform fractions (S2)], with varying aryl-tetralin lignan content were utilized for the present study. The peroxyl ion scavenging potentials of S1 and S2 were found to be comparable [i.e. 45.88% (S1) and 41% (S2)] after a 48 h interval in a time-dependent study, whereas in a 2 h study, S2 exhibited significant (P < 0.05) antioxidant activity in different metal ion + flux states. In the aqueous phase, S2 exhibited non-site-specific reactive oxygen species scavenging activity, i.e. 73.12% inhibition at 500 μg ml−1. S1 exhibited 58.40 ± 0.8% inhibition (at 0.025 μg ml−1) of the formation of reactive nitrite radicals, comparable to S2 (52.45 ± 0.825%), and also showed 45.01% site-specific activity (1000 μg ml−1), along with significant (P < 0.05) electron donation potential (50–2000 μg ml−1) compared to S2. Such activities of S1 could be attributed to the significantly (P < 0.05) higher levels of podophyllotoxin β-d-glucopyranoside (16.5 times) and demethyl podophyllotoxin glucoside (2.9 times) compared with S2. Together, these findings clearly prove that aryl-tetralin lignan content influences the radiation protective potential of the Podophyllum fractions to a great extent. Oxford University Press 2006-12 2006-06-21 /pmc/articles/PMC1697744/ /pubmed/17173115 http://dx.doi.org/10.1093/ecam/nel037 Text en © The Author (2006). Published by Oxford University Press. All rights reserved
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Chawla, Raman Arora, Rajesh Singh, Shikha Sagar, R.K. Sharma, Rakesh Kumar Kumar, R. Sharma, A. Tripathi, R. P. Puri, S. C. Khan, H. A. Shawl, A. S. Sultan, P. Krishan, Tej Qazi, G. N.
spellingShingle	Chawla, Raman Arora, Rajesh Singh, Shikha Sagar, R.K. Sharma, Rakesh Kumar Kumar, R. Sharma, A. Tripathi, R. P. Puri, S. C. Khan, H. A. Shawl, A. S. Sultan, P. Krishan, Tej Qazi, G. N. Podophyllum hexandrum Offers Radioprotection by Modulating Free Radical Flux: Role of Aryl-Tetralin Lignans
author_facet	Chawla, Raman Arora, Rajesh Singh, Shikha Sagar, R.K. Sharma, Rakesh Kumar Kumar, R. Sharma, A. Tripathi, R. P. Puri, S. C. Khan, H. A. Shawl, A. S. Sultan, P. Krishan, Tej Qazi, G. N.
author_sort	Chawla, Raman
title	Podophyllum hexandrum Offers Radioprotection by Modulating Free Radical Flux: Role of Aryl-Tetralin Lignans
title_short	Podophyllum hexandrum Offers Radioprotection by Modulating Free Radical Flux: Role of Aryl-Tetralin Lignans
title_full	Podophyllum hexandrum Offers Radioprotection by Modulating Free Radical Flux: Role of Aryl-Tetralin Lignans
title_fullStr	Podophyllum hexandrum Offers Radioprotection by Modulating Free Radical Flux: Role of Aryl-Tetralin Lignans
title_full_unstemmed	Podophyllum hexandrum Offers Radioprotection by Modulating Free Radical Flux: Role of Aryl-Tetralin Lignans
title_sort	podophyllum hexandrum offers radioprotection by modulating free radical flux: role of aryl-tetralin lignans
description	We have evaluated the effect of variation in aryl-tetralin lignans on the radioprotective properties of Podophyllum hexandrum. Two fractionated fractions of P. hexandrum [methanolic (S1) and chloroform fractions (S2)], with varying aryl-tetralin lignan content were utilized for the present study. The peroxyl ion scavenging potentials of S1 and S2 were found to be comparable [i.e. 45.88% (S1) and 41% (S2)] after a 48 h interval in a time-dependent study, whereas in a 2 h study, S2 exhibited significant (P < 0.05) antioxidant activity in different metal ion + flux states. In the aqueous phase, S2 exhibited non-site-specific reactive oxygen species scavenging activity, i.e. 73.12% inhibition at 500 μg ml−1. S1 exhibited 58.40 ± 0.8% inhibition (at 0.025 μg ml−1) of the formation of reactive nitrite radicals, comparable to S2 (52.45 ± 0.825%), and also showed 45.01% site-specific activity (1000 μg ml−1), along with significant (P < 0.05) electron donation potential (50–2000 μg ml−1) compared to S2. Such activities of S1 could be attributed to the significantly (P < 0.05) higher levels of podophyllotoxin β-d-glucopyranoside (16.5 times) and demethyl podophyllotoxin glucoside (2.9 times) compared with S2. Together, these findings clearly prove that aryl-tetralin lignan content influences the radiation protective potential of the Podophyllum fractions to a great extent.
publisher	Oxford University Press
publishDate	2006
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1697744/
_version_	1611391926964060160

Podophyllum hexandrum Offers Radioprotection by Modulating Free Radical Flux: Role of Aryl-Tetralin Lignans

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