Genomic Convergence toward Diploidy in Saccharomyces cerevisiae

Genome size, a fundamental aspect of any organism, is subject to a variety of mutational and selection pressures. We investigated genome size evolution in haploid, diploid, and tetraploid initially isogenic lines of the yeast Saccharomyces cerevisiae. Over the course of ~1,800 generations of mitotic...

Full description

Bibliographic Details
Main Authors:	Gerstein, Aleeza C, Chun, Hye-Jung E, Grant, Alex, Otto, Sarah P
Format:	Online
Language:	English
Published:	Public Library of Science 2006
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1570378/

id	pubmed-1570378
recordtype	oai_dc
spelling	pubmed-15703782006-10-05 Genomic Convergence toward Diploidy in Saccharomyces cerevisiae Gerstein, Aleeza C Chun, Hye-Jung E Grant, Alex Otto, Sarah P Research Article Genome size, a fundamental aspect of any organism, is subject to a variety of mutational and selection pressures. We investigated genome size evolution in haploid, diploid, and tetraploid initially isogenic lines of the yeast Saccharomyces cerevisiae. Over the course of ~1,800 generations of mitotic division, we observed convergence toward diploid DNA content in all replicate lines. This convergence was observed in both unstressful and stressful environments, although the rate of convergence was dependent on initial ploidy and evolutionary environment. Comparative genomic hybridization with microarrays revealed nearly euploid DNA content by the end of the experiment. As the vegetative life cycle of S. cerevisiae is predominantly diploid, this experiment provides evidence that genome size evolution is constrained, with selection favouring the genomic content typical of the yeast's evolutionary past. Public Library of Science 2006-09 2006-09-22 /pmc/articles/PMC1570378/ /pubmed/17002497 http://dx.doi.org/10.1371/journal.pgen.0020145 Text en © 2006 Gerstein et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Gerstein, Aleeza C Chun, Hye-Jung E Grant, Alex Otto, Sarah P
spellingShingle	Gerstein, Aleeza C Chun, Hye-Jung E Grant, Alex Otto, Sarah P Genomic Convergence toward Diploidy in Saccharomyces cerevisiae
author_facet	Gerstein, Aleeza C Chun, Hye-Jung E Grant, Alex Otto, Sarah P
author_sort	Gerstein, Aleeza C
title	Genomic Convergence toward Diploidy in Saccharomyces cerevisiae
title_short	Genomic Convergence toward Diploidy in Saccharomyces cerevisiae
title_full	Genomic Convergence toward Diploidy in Saccharomyces cerevisiae
title_fullStr	Genomic Convergence toward Diploidy in Saccharomyces cerevisiae
title_full_unstemmed	Genomic Convergence toward Diploidy in Saccharomyces cerevisiae
title_sort	genomic convergence toward diploidy in saccharomyces cerevisiae
description	Genome size, a fundamental aspect of any organism, is subject to a variety of mutational and selection pressures. We investigated genome size evolution in haploid, diploid, and tetraploid initially isogenic lines of the yeast Saccharomyces cerevisiae. Over the course of ~1,800 generations of mitotic division, we observed convergence toward diploid DNA content in all replicate lines. This convergence was observed in both unstressful and stressful environments, although the rate of convergence was dependent on initial ploidy and evolutionary environment. Comparative genomic hybridization with microarrays revealed nearly euploid DNA content by the end of the experiment. As the vegetative life cycle of S. cerevisiae is predominantly diploid, this experiment provides evidence that genome size evolution is constrained, with selection favouring the genomic content typical of the yeast's evolutionary past.
publisher	Public Library of Science
publishDate	2006
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1570378/
_version_	1611389337952321536

Genomic Convergence toward Diploidy in Saccharomyces cerevisiae

Similar Items