Comparison of the toxic effects of hydrogen peroxide and ozone on cultured human bronchial epithelial cells.

In this study, we compared the cytotoxic and genotoxic effects of hydrogen peroxide and ozone on cultured human airway epithelial cells in primary culture. Both agents caused a dose-dependent loss in the replicative ability of epithelial cells and at higher levels of exposure caused acute cytotoxici...

Full description

Bibliographic Details
Main Authors:	Gabrielson, E W, Yu, X Y, Spannhake, E W
Format:	Online
Language:	English
Published:	1994
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567440/

id	pubmed-1567440
recordtype	oai_dc
spelling	pubmed-15674402006-09-19 Comparison of the toxic effects of hydrogen peroxide and ozone on cultured human bronchial epithelial cells. Gabrielson, E W Yu, X Y Spannhake, E W Research Article In this study, we compared the cytotoxic and genotoxic effects of hydrogen peroxide and ozone on cultured human airway epithelial cells in primary culture. Both agents caused a dose-dependent loss in the replicative ability of epithelial cells and at higher levels of exposure caused acute cytotoxicity as measured by release of lactate dehydrogenase. Differences were seen, however, between the agents' effects with regard to induction of DNA single strand breaks as measured by alkaline elution:; whereas single-strand breaks were detected in significant amounts at concentration of hydrogen peroxide that cause acute cytotoxicity, none were detected at any of the levels of ozone exposure examined. A difference was also seen in the ability of the iron chelator deferoxamine to protect cells from the effect of the two oxidants. Preincubation of cultures with deferoxamine appreciably attenuated the toxicity of hydrogen peroxide but not of ozone. These data suggest that ozone has significant toxic effects on bronchial epithelial cells not mediated through the generation of hydrogen peroxide or hydroxyl radical. Furthermore, the data indicate that the inhibiting action of ozone on cell replicative ability is not mediated through a mechanism related to DNA single strand breaks. 1994-11 /pmc/articles/PMC1567440/ /pubmed/9738213 Text en
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Gabrielson, E W Yu, X Y Spannhake, E W
spellingShingle	Gabrielson, E W Yu, X Y Spannhake, E W Comparison of the toxic effects of hydrogen peroxide and ozone on cultured human bronchial epithelial cells.
author_facet	Gabrielson, E W Yu, X Y Spannhake, E W
author_sort	Gabrielson, E W
title	Comparison of the toxic effects of hydrogen peroxide and ozone on cultured human bronchial epithelial cells.
title_short	Comparison of the toxic effects of hydrogen peroxide and ozone on cultured human bronchial epithelial cells.
title_full	Comparison of the toxic effects of hydrogen peroxide and ozone on cultured human bronchial epithelial cells.
title_fullStr	Comparison of the toxic effects of hydrogen peroxide and ozone on cultured human bronchial epithelial cells.
title_full_unstemmed	Comparison of the toxic effects of hydrogen peroxide and ozone on cultured human bronchial epithelial cells.
title_sort	comparison of the toxic effects of hydrogen peroxide and ozone on cultured human bronchial epithelial cells.
description	In this study, we compared the cytotoxic and genotoxic effects of hydrogen peroxide and ozone on cultured human airway epithelial cells in primary culture. Both agents caused a dose-dependent loss in the replicative ability of epithelial cells and at higher levels of exposure caused acute cytotoxicity as measured by release of lactate dehydrogenase. Differences were seen, however, between the agents' effects with regard to induction of DNA single strand breaks as measured by alkaline elution:; whereas single-strand breaks were detected in significant amounts at concentration of hydrogen peroxide that cause acute cytotoxicity, none were detected at any of the levels of ozone exposure examined. A difference was also seen in the ability of the iron chelator deferoxamine to protect cells from the effect of the two oxidants. Preincubation of cultures with deferoxamine appreciably attenuated the toxicity of hydrogen peroxide but not of ozone. These data suggest that ozone has significant toxic effects on bronchial epithelial cells not mediated through the generation of hydrogen peroxide or hydroxyl radical. Furthermore, the data indicate that the inhibiting action of ozone on cell replicative ability is not mediated through a mechanism related to DNA single strand breaks.
publishDate	1994
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567440/
_version_	1611388203838734336

Comparison of the toxic effects of hydrogen peroxide and ozone on cultured human bronchial epithelial cells.

Similar Items