Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels
An inexpensive Plexiglas apparatus which allows a simple and rapid preparation of horizontal polyacrylamide gels of different dimensions for different purposes, is described. Preparation of such gels is as easy and rapid as agarose gel preparation, and polymerized polyacrylamide gels are used to fra...
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| Format: | Online |
| Language: | English |
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BioMed Central
2006
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1538630/ |
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pubmed-1538630 |
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pubmed-15386302006-08-10 Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels Izzo, Vincenzo Costa, Maria A Di Fiore, Renata Duro, Giovanni Bellavia, Daniele Cascone, Eleonora Colombo, Paolo Gioviale, Maria C Barbieri, Rainer Methodology An inexpensive Plexiglas apparatus which allows a simple and rapid preparation of horizontal polyacrylamide gels of different dimensions for different purposes, is described. Preparation of such gels is as easy and rapid as agarose gel preparation, and polymerized polyacrylamide gels are used to fractionate proteins or small DNA fragments using a common horizontal electrophoretic tank. This apparatus was used to electrophoretically fractionate proteins or DNA for immuno-blot analyses, particularirly in the study of the allergenic response to Parietaria judaica pollen in senescence, for Southern-blot hybridizations and in the study of DNA polymorphisms. BioMed Central 2006-07-12 /pmc/articles/PMC1538630/ /pubmed/16836743 http://dx.doi.org/10.1186/1742-4933-3-7 Text en Copyright © 2006 Izzo et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Izzo, Vincenzo Costa, Maria A Di Fiore, Renata Duro, Giovanni Bellavia, Daniele Cascone, Eleonora Colombo, Paolo Gioviale, Maria C Barbieri, Rainer |
| spellingShingle |
Izzo, Vincenzo Costa, Maria A Di Fiore, Renata Duro, Giovanni Bellavia, Daniele Cascone, Eleonora Colombo, Paolo Gioviale, Maria C Barbieri, Rainer Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels |
| author_facet |
Izzo, Vincenzo Costa, Maria A Di Fiore, Renata Duro, Giovanni Bellavia, Daniele Cascone, Eleonora Colombo, Paolo Gioviale, Maria C Barbieri, Rainer |
| author_sort |
Izzo, Vincenzo |
| title |
Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels |
| title_short |
Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels |
| title_full |
Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels |
| title_fullStr |
Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels |
| title_full_unstemmed |
Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels |
| title_sort |
electrophoresis of proteins and dna on horizontal sodium dodecyl sulfate polyacrylamide gels |
| description |
An inexpensive Plexiglas apparatus which allows a simple and rapid preparation of horizontal polyacrylamide gels of different dimensions for different purposes, is described. Preparation of such gels is as easy and rapid as agarose gel preparation, and polymerized polyacrylamide gels are used to fractionate proteins or small DNA fragments using a common horizontal electrophoretic tank. This apparatus was used to electrophoretically fractionate proteins or DNA for immuno-blot analyses, particularirly in the study of the allergenic response to Parietaria judaica pollen in senescence, for Southern-blot hybridizations and in the study of DNA polymorphisms. |
| publisher |
BioMed Central |
| publishDate |
2006 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1538630/ |
| _version_ |
1611386375328759808 |