Pyrene binary probes for unambiguous detection of mRNA using time-resolved fluorescence spectroscopy
We report here the design, synthesis and application of pyrene binary oligonucleotide probes for selective detection of cellular mRNA. The detection strategy is based on the formation of a fluorescent excimer when two pyrene groups are brought into close proximity upon hybridization of the probes wi...
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| Format: | Online |
| Language: | English |
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Oxford University Press
2006
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1477857/ |
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pubmed-1477857 |
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pubmed-14778572006-06-26 Pyrene binary probes for unambiguous detection of mRNA using time-resolved fluorescence spectroscopy Martí, Angel A. Li, Xiaoxu Jockusch, Steffen Li, Zengmin Raveendra, Bindu Kalachikov, Sergey Russo, James J. Morozova, Irina Puthanveettil, Sathyanarayanan V. Ju, Jingyue Turro, Nicholas J. Article We report here the design, synthesis and application of pyrene binary oligonucleotide probes for selective detection of cellular mRNA. The detection strategy is based on the formation of a fluorescent excimer when two pyrene groups are brought into close proximity upon hybridization of the probes with the target mRNA. The pyrene excimer has a long fluorescence lifetime (>40 ns) compared with that of cellular extracts (∼7 ns), allowing selective detection of the excimer using time-resolved emission spectra (TRES). Optimized probes were used to target a specific region of sensorin mRNA yielding a strong excimer emission peak at 485 nm in the presence of the target and no excimer emission in the absence of the target in buffer solution. While direct fluorescence measurement of neuronal extracts showed a strong fluorescent background, obscuring the detection of the excimer signal, time-resolved emission measurements indicated that the emission decay of the cellular extracts is ∼8 times faster than that of the pyrene excimer probes. Thus, using TRES of the pyrene probes, we are able to selectively detect mRNA in the presence of cellular extracts, demonstrating the potential for application of pyrene excimer probes for imaging mRNAs in cellular environments that have background fluorescence. Oxford University Press 2006 2006-06-12 /pmc/articles/PMC1477857/ /pubmed/16769776 http://dx.doi.org/10.1093/nar/gkl406 Text en © 2006 The Author(s) |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Martí, Angel A. Li, Xiaoxu Jockusch, Steffen Li, Zengmin Raveendra, Bindu Kalachikov, Sergey Russo, James J. Morozova, Irina Puthanveettil, Sathyanarayanan V. Ju, Jingyue Turro, Nicholas J. |
| spellingShingle |
Martí, Angel A. Li, Xiaoxu Jockusch, Steffen Li, Zengmin Raveendra, Bindu Kalachikov, Sergey Russo, James J. Morozova, Irina Puthanveettil, Sathyanarayanan V. Ju, Jingyue Turro, Nicholas J. Pyrene binary probes for unambiguous detection of mRNA using time-resolved fluorescence spectroscopy |
| author_facet |
Martí, Angel A. Li, Xiaoxu Jockusch, Steffen Li, Zengmin Raveendra, Bindu Kalachikov, Sergey Russo, James J. Morozova, Irina Puthanveettil, Sathyanarayanan V. Ju, Jingyue Turro, Nicholas J. |
| author_sort |
Martí, Angel A. |
| title |
Pyrene binary probes for unambiguous detection of mRNA using time-resolved fluorescence spectroscopy |
| title_short |
Pyrene binary probes for unambiguous detection of mRNA using time-resolved fluorescence spectroscopy |
| title_full |
Pyrene binary probes for unambiguous detection of mRNA using time-resolved fluorescence spectroscopy |
| title_fullStr |
Pyrene binary probes for unambiguous detection of mRNA using time-resolved fluorescence spectroscopy |
| title_full_unstemmed |
Pyrene binary probes for unambiguous detection of mRNA using time-resolved fluorescence spectroscopy |
| title_sort |
pyrene binary probes for unambiguous detection of mrna using time-resolved fluorescence spectroscopy |
| description |
We report here the design, synthesis and application of pyrene binary oligonucleotide probes for selective detection of cellular mRNA. The detection strategy is based on the formation of a fluorescent excimer when two pyrene groups are brought into close proximity upon hybridization of the probes with the target mRNA. The pyrene excimer has a long fluorescence lifetime (>40 ns) compared with that of cellular extracts (∼7 ns), allowing selective detection of the excimer using time-resolved emission spectra (TRES). Optimized probes were used to target a specific region of sensorin mRNA yielding a strong excimer emission peak at 485 nm in the presence of the target and no excimer emission in the absence of the target in buffer solution. While direct fluorescence measurement of neuronal extracts showed a strong fluorescent background, obscuring the detection of the excimer signal, time-resolved emission measurements indicated that the emission decay of the cellular extracts is ∼8 times faster than that of the pyrene excimer probes. Thus, using TRES of the pyrene probes, we are able to selectively detect mRNA in the presence of cellular extracts, demonstrating the potential for application of pyrene excimer probes for imaging mRNAs in cellular environments that have background fluorescence. |
| publisher |
Oxford University Press |
| publishDate |
2006 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1477857/ |
| _version_ |
1611384044763742208 |