Human hypervariable sequences in risk assessment: rare Ha-ras alleles in cancer patients.

Human hypervariable sequences in risk assessment: rare Ha-ras alleles in cancer patients.

A variable tandem repeat (VTR) is responsible for the hyperallelism one kilobase 3' to the human c-Ha-ras-1 (Ha-ras) gene. Thirty-two distinct restriction fragments, comprising 3 allelic classes by frequency of occurrence, have thus far been detected in a sample size of approximately 800 caucas...

Full description

Bibliographic Details
Main Authors: Krontiris, T G, DiMartino, N A, Mitcheson, H D, Lonergan, J A, Begg, C, Parkinson, D R
Format: Online
Language:English
Published: 1987
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1474469/
id pubmed-1474469
recordtype oai_dc
spelling pubmed-14744692006-06-09 Human hypervariable sequences in risk assessment: rare Ha-ras alleles in cancer patients. Krontiris, T G DiMartino, N A Mitcheson, H D Lonergan, J A Begg, C Parkinson, D R Research Article A variable tandem repeat (VTR) is responsible for the hyperallelism one kilobase 3' to the human c-Ha-ras-1 (Ha-ras) gene. Thirty-two distinct restriction fragments, comprising 3 allelic classes by frequency of occurrence, have thus far been detected in a sample size of approximately 800 caucasians. Rare Ha-ras alleles, 21 in all, are almost exclusively confined to the genomes of cancer patients (p less than 0.001). From our data we have computed the relative cancer risk associated with possession of a rare Ha-ras allele to be 27. To understand the molecular basis for this phenomenon, we have begun to clone Ha-ras fragments from nontumor DNA of cancer patients. We report here the weak activation, as detected by transfection and transformation of NIH 3T3 mouse cells, of two Ha-ras genes which were obtained from lymphocyte DNA of a melanoma patient. We have mapped the regions that confer this transforming activity to the fragment containing the VTR in one Ha-ras clone and the fragment containing gene coding sequences in the other. 1987-12 /pmc/articles/PMC1474469/ /pubmed/3329095 Text en
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Krontiris, T G
DiMartino, N A
Mitcheson, H D
Lonergan, J A
Begg, C
Parkinson, D R
spellingShingle Krontiris, T G
DiMartino, N A
Mitcheson, H D
Lonergan, J A
Begg, C
Parkinson, D R
Human hypervariable sequences in risk assessment: rare Ha-ras alleles in cancer patients.
author_facet Krontiris, T G
DiMartino, N A
Mitcheson, H D
Lonergan, J A
Begg, C
Parkinson, D R
author_sort Krontiris, T G
title Human hypervariable sequences in risk assessment: rare Ha-ras alleles in cancer patients.
title_short Human hypervariable sequences in risk assessment: rare Ha-ras alleles in cancer patients.
title_full Human hypervariable sequences in risk assessment: rare Ha-ras alleles in cancer patients.
title_fullStr Human hypervariable sequences in risk assessment: rare Ha-ras alleles in cancer patients.
title_full_unstemmed Human hypervariable sequences in risk assessment: rare Ha-ras alleles in cancer patients.
title_sort human hypervariable sequences in risk assessment: rare ha-ras alleles in cancer patients.
description A variable tandem repeat (VTR) is responsible for the hyperallelism one kilobase 3' to the human c-Ha-ras-1 (Ha-ras) gene. Thirty-two distinct restriction fragments, comprising 3 allelic classes by frequency of occurrence, have thus far been detected in a sample size of approximately 800 caucasians. Rare Ha-ras alleles, 21 in all, are almost exclusively confined to the genomes of cancer patients (p less than 0.001). From our data we have computed the relative cancer risk associated with possession of a rare Ha-ras allele to be 27. To understand the molecular basis for this phenomenon, we have begun to clone Ha-ras fragments from nontumor DNA of cancer patients. We report here the weak activation, as detected by transfection and transformation of NIH 3T3 mouse cells, of two Ha-ras genes which were obtained from lymphocyte DNA of a melanoma patient. We have mapped the regions that confer this transforming activity to the fragment containing the VTR in one Ha-ras clone and the fragment containing gene coding sequences in the other.
publishDate 1987
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1474469/
_version_ 1611383412987265024

Similar Items