Biological monitoring of genotoxic hazard in workers of the rubber industry.

Biological monitoring of genotoxic hazard in the rubber industry was performed in 19 male workers and 20 age-matched controls in a local health unit in northern Italy. Peripheral blood lymphocytes were analyzed for the presence of DNA damage (single-cell microgel-electrophoresis, or comet assay) and...

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Main Authors:	Moretti, M, Villarini, M, Scassellati-Sforzolini, G, Monarca, S, Libraro, M, Fatigoni, C, Donato, F, Leonardis, C, Perego, L
Format:	Online
Language:	English
Published:	1996
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1469638/

id	pubmed-1469638
recordtype	oai_dc
spelling	pubmed-14696382006-06-01 Biological monitoring of genotoxic hazard in workers of the rubber industry. Moretti, M Villarini, M Scassellati-Sforzolini, G Monarca, S Libraro, M Fatigoni, C Donato, F Leonardis, C Perego, L Research Article Biological monitoring of genotoxic hazard in the rubber industry was performed in 19 male workers and 20 age-matched controls in a local health unit in northern Italy. Peripheral blood lymphocytes were analyzed for the presence of DNA damage (single-cell microgel-electrophoresis, or comet assay) and for cytogenetic parameters (sister chromatid exchanges and micronuclei frequency, and proliferative rate index). The following bioassays were performed in urine samples: a) mutagenicity test and concentration of thioethers as markers of exposure, and b) excretion of D-glucaric acid and 6-beta-hydroxycortisol (related to 17-hydroxycorticosteroid excretion) as indicators of the inductive status of the microsomal enzyme system (phase-I). The exposed subjects showed statistically higher mean values of 17-hydroxycorticosteroids and micronuclei and lower values of 6-beta-hydroxycortisol than controls, when taking cigarette smoking into account. The comet assay showed higher values for migration distance in exposed subjects than controls, although the differences were not significant at a p-value of 0.05. These findings suggest that industrial exposure in the rubber processing industry may cause genetic damage and may modify the activity level of some enzymes; these results should be considered with caution due to the small number of subjects enrolled. 1996-05 /pmc/articles/PMC1469638/ /pubmed/8781380 Text en
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Moretti, M Villarini, M Scassellati-Sforzolini, G Monarca, S Libraro, M Fatigoni, C Donato, F Leonardis, C Perego, L
spellingShingle	Moretti, M Villarini, M Scassellati-Sforzolini, G Monarca, S Libraro, M Fatigoni, C Donato, F Leonardis, C Perego, L Biological monitoring of genotoxic hazard in workers of the rubber industry.
author_facet	Moretti, M Villarini, M Scassellati-Sforzolini, G Monarca, S Libraro, M Fatigoni, C Donato, F Leonardis, C Perego, L
author_sort	Moretti, M
title	Biological monitoring of genotoxic hazard in workers of the rubber industry.
title_short	Biological monitoring of genotoxic hazard in workers of the rubber industry.
title_full	Biological monitoring of genotoxic hazard in workers of the rubber industry.
title_fullStr	Biological monitoring of genotoxic hazard in workers of the rubber industry.
title_full_unstemmed	Biological monitoring of genotoxic hazard in workers of the rubber industry.
title_sort	biological monitoring of genotoxic hazard in workers of the rubber industry.
description	Biological monitoring of genotoxic hazard in the rubber industry was performed in 19 male workers and 20 age-matched controls in a local health unit in northern Italy. Peripheral blood lymphocytes were analyzed for the presence of DNA damage (single-cell microgel-electrophoresis, or comet assay) and for cytogenetic parameters (sister chromatid exchanges and micronuclei frequency, and proliferative rate index). The following bioassays were performed in urine samples: a) mutagenicity test and concentration of thioethers as markers of exposure, and b) excretion of D-glucaric acid and 6-beta-hydroxycortisol (related to 17-hydroxycorticosteroid excretion) as indicators of the inductive status of the microsomal enzyme system (phase-I). The exposed subjects showed statistically higher mean values of 17-hydroxycorticosteroids and micronuclei and lower values of 6-beta-hydroxycortisol than controls, when taking cigarette smoking into account. The comet assay showed higher values for migration distance in exposed subjects than controls, although the differences were not significant at a p-value of 0.05. These findings suggest that industrial exposure in the rubber processing industry may cause genetic damage and may modify the activity level of some enzymes; these results should be considered with caution due to the small number of subjects enrolled.
publishDate	1996
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1469638/
_version_	1611382765307035648

Biological monitoring of genotoxic hazard in workers of the rubber industry.

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