Effects of Extracellular Matrix on the Morphology and Behaviour of Rabbit Auricular Chondrocytes in Culture
Isolated chondrocytes dedifferentiate to a fibroblast-like shape on plastic substrata and proliferate extensively, but rarely form nodules. However, when dissociation is not complete and some cartilage remnants are included in the culture, proliferation decreases and cells grow in a reticular patter...
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| Format: | Online |
| Language: | English |
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Hindawi Publishing Corporation
2005
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1361486/ |
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pubmed-1361486 |
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pubmed-13614862006-02-22 Effects of Extracellular Matrix on the Morphology and Behaviour of Rabbit Auricular Chondrocytes in Culture Villar-Suárez, Vega Colaço, B. Calles-Venal, I. Bravo, I. G. Fernández-Álvarez, J. G. Fernández-Caso, M. Villar-Lacilla, J. M. Research Article Isolated chondrocytes dedifferentiate to a fibroblast-like shape on plastic substrata and proliferate extensively, but rarely form nodules. However, when dissociation is not complete and some cartilage remnants are included in the culture, proliferation decreases and cells grow in a reticular pattern with numerous nodules, which occasionally form small cartilage-like fragments. In an attempt to reproduce this stable chondrogenic state, we added a cartilage protein extract, a sugar extract, and hyaluronan to the medium of previously dedifferentiated chondrocytes. When protein extract was added, many cartilaginous nodules appeared. Hyaluronan produced changes in cell phenotype and behaviour, but not nodule formation. Protein extract has positive effects on the differentiation of previously proliferated chondrocytes and permits nodule formation and the extensive production of type-II collagen. A comparison with incompletely dissociated chondrocyte cultures suggests that the presence of some living cells anchored to their natural extracellular matrix provides some important additional factors for the phenotypical stability of chondrocytes on plastic surfaces. In order to elucidate if it is possible that the incidence of apoptosis is related to the results, we also characterized the molecular traits of apoptosis. Hindawi Publishing Corporation 2005 /pmc/articles/PMC1361486/ /pubmed/16489269 http://dx.doi.org/10.1155/JBB.2005.364 Text en Vega Villar-Suárez et al |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Villar-Suárez, Vega Colaço, B. Calles-Venal, I. Bravo, I. G. Fernández-Álvarez, J. G. Fernández-Caso, M. Villar-Lacilla, J. M. |
| spellingShingle |
Villar-Suárez, Vega Colaço, B. Calles-Venal, I. Bravo, I. G. Fernández-Álvarez, J. G. Fernández-Caso, M. Villar-Lacilla, J. M. Effects of Extracellular Matrix on the Morphology and Behaviour of Rabbit Auricular Chondrocytes in Culture |
| author_facet |
Villar-Suárez, Vega Colaço, B. Calles-Venal, I. Bravo, I. G. Fernández-Álvarez, J. G. Fernández-Caso, M. Villar-Lacilla, J. M. |
| author_sort |
Villar-Suárez, Vega |
| title |
Effects of Extracellular Matrix on the Morphology and
Behaviour of Rabbit Auricular Chondrocytes in Culture |
| title_short |
Effects of Extracellular Matrix on the Morphology and
Behaviour of Rabbit Auricular Chondrocytes in Culture |
| title_full |
Effects of Extracellular Matrix on the Morphology and
Behaviour of Rabbit Auricular Chondrocytes in Culture |
| title_fullStr |
Effects of Extracellular Matrix on the Morphology and
Behaviour of Rabbit Auricular Chondrocytes in Culture |
| title_full_unstemmed |
Effects of Extracellular Matrix on the Morphology and
Behaviour of Rabbit Auricular Chondrocytes in Culture |
| title_sort |
effects of extracellular matrix on the morphology and
behaviour of rabbit auricular chondrocytes in culture |
| description |
Isolated chondrocytes dedifferentiate to a
fibroblast-like shape on plastic substrata and proliferate
extensively, but rarely form nodules. However, when dissociation
is not complete and some cartilage remnants are included in the
culture, proliferation decreases and cells grow in a reticular
pattern with numerous nodules, which occasionally form small
cartilage-like fragments. In an attempt to reproduce this stable
chondrogenic state, we added a cartilage protein extract, a sugar
extract, and hyaluronan to the medium of previously
dedifferentiated chondrocytes. When protein extract was added,
many cartilaginous nodules appeared. Hyaluronan produced changes
in cell phenotype and behaviour, but not nodule formation. Protein
extract has positive effects on the differentiation of previously
proliferated chondrocytes and permits nodule formation and the
extensive production of type-II collagen. A comparison with
incompletely dissociated chondrocyte cultures suggests that the
presence of some living cells anchored to their natural
extracellular matrix provides some important additional factors
for the phenotypical stability of chondrocytes on plastic
surfaces. In order to elucidate if it is possible that the
incidence of apoptosis is related to the results, we also
characterized the molecular traits of apoptosis. |
| publisher |
Hindawi Publishing Corporation |
| publishDate |
2005 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1361486/ |
| _version_ |
1611380581687361536 |