Molecular characterization of geminivirus-derived small RNAs in different plant species
DNA geminiviruses are thought to be targets of RNA silencing. Here, we characterize small interfering (si) RNAs—the hallmarks of silencing—associated with Cabbage leaf curl begomovirus in Arabidopsis and African cassava mosaic begomovirus in Nicotiana benthamiana and cassava. We detected 21, 22 and...
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| Format: | Online |
| Language: | English |
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Oxford University Press
2006
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1342034/ |
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pubmed-1342034 |
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pubmed-13420342006-01-23 Molecular characterization of geminivirus-derived small RNAs in different plant species Akbergenov, Rashid Si-Ammour, Azeddine Blevins, Todd Amin, Imran Kutter, Claudia Vanderschuren, Herve Zhang, Peng Gruissem, Wilhelm Meins, Frederick Hohn, Thomas Pooggin, Mikhail M. Article DNA geminiviruses are thought to be targets of RNA silencing. Here, we characterize small interfering (si) RNAs—the hallmarks of silencing—associated with Cabbage leaf curl begomovirus in Arabidopsis and African cassava mosaic begomovirus in Nicotiana benthamiana and cassava. We detected 21, 22 and 24 nt siRNAs of both polarities, derived from both the coding and the intergenic regions of these geminiviruses. Genetic evidence showed that all the 24 nt and a substantial fraction of the 22 nt viral siRNAs are generated by the dicer-like proteins DCL3 and DCL2, respectively. The viral siRNAs were 5′ end phosphorylated, as shown by phosphatase treatments, and methylated at the 3′-nucleotide, as shown by HEN1 miRNA methylase-dependent resistance to β-elimination. Similar modifications were found in all types of endogenous and transgene-derived siRNAs tested, but not in a major fraction of siRNAs from a cytoplasmic RNA tobamovirus. We conclude that several distinct silencing pathways are involved in DNA virus-plant interactions. Oxford University Press 2006 2006-01-18 /pmc/articles/PMC1342034/ /pubmed/16421273 http://dx.doi.org/10.1093/nar/gkj447 Text en © The Author 2006. Published by Oxford University Press. All rights reserved |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Akbergenov, Rashid Si-Ammour, Azeddine Blevins, Todd Amin, Imran Kutter, Claudia Vanderschuren, Herve Zhang, Peng Gruissem, Wilhelm Meins, Frederick Hohn, Thomas Pooggin, Mikhail M. |
| spellingShingle |
Akbergenov, Rashid Si-Ammour, Azeddine Blevins, Todd Amin, Imran Kutter, Claudia Vanderschuren, Herve Zhang, Peng Gruissem, Wilhelm Meins, Frederick Hohn, Thomas Pooggin, Mikhail M. Molecular characterization of geminivirus-derived small RNAs in different plant species |
| author_facet |
Akbergenov, Rashid Si-Ammour, Azeddine Blevins, Todd Amin, Imran Kutter, Claudia Vanderschuren, Herve Zhang, Peng Gruissem, Wilhelm Meins, Frederick Hohn, Thomas Pooggin, Mikhail M. |
| author_sort |
Akbergenov, Rashid |
| title |
Molecular characterization of geminivirus-derived small RNAs in different plant species |
| title_short |
Molecular characterization of geminivirus-derived small RNAs in different plant species |
| title_full |
Molecular characterization of geminivirus-derived small RNAs in different plant species |
| title_fullStr |
Molecular characterization of geminivirus-derived small RNAs in different plant species |
| title_full_unstemmed |
Molecular characterization of geminivirus-derived small RNAs in different plant species |
| title_sort |
molecular characterization of geminivirus-derived small rnas in different plant species |
| description |
DNA geminiviruses are thought to be targets of RNA silencing. Here, we characterize small interfering (si) RNAs—the hallmarks of silencing—associated with Cabbage leaf curl begomovirus in Arabidopsis and African cassava mosaic begomovirus in Nicotiana benthamiana and cassava. We detected 21, 22 and 24 nt siRNAs of both polarities, derived from both the coding and the intergenic regions of these geminiviruses. Genetic evidence showed that all the 24 nt and a substantial fraction of the 22 nt viral siRNAs are generated by the dicer-like proteins DCL3 and DCL2, respectively. The viral siRNAs were 5′ end phosphorylated, as shown by phosphatase treatments, and methylated at the 3′-nucleotide, as shown by HEN1 miRNA methylase-dependent resistance to β-elimination. Similar modifications were found in all types of endogenous and transgene-derived siRNAs tested, but not in a major fraction of siRNAs from a cytoplasmic RNA tobamovirus. We conclude that several distinct silencing pathways are involved in DNA virus-plant interactions. |
| publisher |
Oxford University Press |
| publishDate |
2006 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1342034/ |
| _version_ |
1611380267083104256 |