Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange

Comparative analysis of mutants using transfection is complicated by clones exhibiting variable levels of gene expression due to copy number differences and genomic position effects. Recombinase-mediated cassette exchange (RMCE) can overcome these problems by introducing the target gene into pre-det...

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Bibliographic Details
Main Authors: Wong, Ee Tsin, Kolman, John L, Li, Yao-Cheng, Mesner, Larry D, Hillen, Wolfgang, Berens, Christian, Wahl, Geoffrey M
Format: Online
Language:English
Published: Oxford University Press 2005
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1243804/