Electrochemically directed synthesis of oligonucleotides for DNA microarray fabrication
We demonstrate a new method for making oligonucleotide microarrays by synthesis in situ. The method uses conventional DNA synthesis chemistry with an electrochemical deblocking step. Acid is delivered to specific regions on a glass slide, thus allowing nucleotide addition only at chosen sites. The a...
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| Format: | Online |
| Language: | English |
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Oxford University Press
2005
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1183109/ |
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pubmed-1183109 |
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pubmed-11831092005-08-05 Electrochemically directed synthesis of oligonucleotides for DNA microarray fabrication Egeland, Ryan D. Southern, Edwin M. Methods Online We demonstrate a new method for making oligonucleotide microarrays by synthesis in situ. The method uses conventional DNA synthesis chemistry with an electrochemical deblocking step. Acid is delivered to specific regions on a glass slide, thus allowing nucleotide addition only at chosen sites. The acid is produced by electrochemical oxidation controlled by an array of independent microelectrodes. Deblocking is complete in a few seconds, when competing side-product reactions are minimal. We demonstrate the successful synthesis of 17mers and discrimination of single base pair mismatched hybrids. Features generated in this study are 40 μm wide, with sharply defined edges. The synthetic technique may be applicable to fabrication of other molecular arrays. Oxford University Press 2005 2005-08-05 /pmc/articles/PMC1183109/ /pubmed/16085751 http://dx.doi.org/10.1093/nar/gni117 Text en © The Author 2005. Published by Oxford University Press. All rights reserved |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Egeland, Ryan D. Southern, Edwin M. |
| spellingShingle |
Egeland, Ryan D. Southern, Edwin M. Electrochemically directed synthesis of oligonucleotides for DNA microarray fabrication |
| author_facet |
Egeland, Ryan D. Southern, Edwin M. |
| author_sort |
Egeland, Ryan D. |
| title |
Electrochemically directed synthesis of oligonucleotides for DNA microarray fabrication |
| title_short |
Electrochemically directed synthesis of oligonucleotides for DNA microarray fabrication |
| title_full |
Electrochemically directed synthesis of oligonucleotides for DNA microarray fabrication |
| title_fullStr |
Electrochemically directed synthesis of oligonucleotides for DNA microarray fabrication |
| title_full_unstemmed |
Electrochemically directed synthesis of oligonucleotides for DNA microarray fabrication |
| title_sort |
electrochemically directed synthesis of oligonucleotides for dna microarray fabrication |
| description |
We demonstrate a new method for making oligonucleotide microarrays by synthesis in situ. The method uses conventional DNA synthesis chemistry with an electrochemical deblocking step. Acid is delivered to specific regions on a glass slide, thus allowing nucleotide addition only at chosen sites. The acid is produced by electrochemical oxidation controlled by an array of independent microelectrodes. Deblocking is complete in a few seconds, when competing side-product reactions are minimal. We demonstrate the successful synthesis of 17mers and discrimination of single base pair mismatched hybrids. Features generated in this study are 40 μm wide, with sharply defined edges. The synthetic technique may be applicable to fabrication of other molecular arrays. |
| publisher |
Oxford University Press |
| publishDate |
2005 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1183109/ |
| _version_ |
1611376134712197120 |