Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3? (Ser-9) in liver

Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3? (Ser-9) in liver

Gynura procumbens, a medicinal plant locally known as Sambung nyawa, is widely used traditionally in South East Asia as a remedy to alleviate symptoms of various ailments including diabetes mellitus. Previous investigators reported that the ethanol plant extract was hypoglycemic in streptozotocin (S...

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Main Author: Tuan Norhafizah binti Tuan Zakaria
Other Authors: Chong, Ching June
Format: Journal
Published: Sains Malaysiana, Penerbit Universiti Kebangsaan Malaysia 2012
Subjects:
Life sciences
Online Access:http://www.myjurnal.my/public/article-view.php?id=65095
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spelling oai:www.myjurnal.my:650952018-09-20T00:00:00Z Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3? (Ser-9) in liver Tuan Norhafizah binti Tuan Zakaria Life sciences Gynura procumbens, a medicinal plant locally known as Sambung nyawa, is widely used traditionally in South East Asia as a remedy to alleviate symptoms of various ailments including diabetes mellitus. Previous investigators reported that the ethanol plant extract was hypoglycemic in streptozotocin (STZ)-induced diabetic rats and suggested the blood glucose-reducing effect could be due to G. procumbens mimicking insulin effects. Our present study aimed to reevaluate the hypoglycemic effect(s) of G. procumbens and to ascertain the involvement of glycogen synthase kinase (GSK3), a key component of insulin biosignaling in the plant activity. A cell-based assay showed that the three G. procumbens fractions tested did not exhibit anti-GSK3 activities. Oral administrations of hexane, ethyl acetate, n-butanol fractions of G. procumbens (250 mg/kg b.w.) and Glibenclamide (5 mg/kg b.w.) for 14 days in STZ-induced diabetic rats reduced blood glucose levels by 29.7%, 60.1%, 33.5% and 61.7% (p<0.05), respectively compared to diabetic control. Liver glycogen contents were elevated (p<0.05), following administration of the three fractions of G. procumbens in diabetic rats when analysed after 14 days of treatment. Western blot analysis also showed GSK3? in the liver of G. procumbens fractions-treated and Glibenclamide-treated animals were phosphorylated at Ser-9. The above findings indicated that the hypoglycemic action of G. procumbens fractions resulted in the inactivation of GSK3? in liver of diabetic rats. Since G. procumbens fractions did not exhibit GSK3 inhibitory properties, it is possible that the hypoglycemic action of the fractions observed here could be due to direct or indirect effects on upstream components of the insulin biosignaling pathway. Sains Malaysiana, Penerbit Universiti Kebangsaan Malaysia Chong, Ching June 2012-00-00 Journal application/pdf 65095 www.myjurnal.my/filebank/published_article/17445JSM_2012_Chong.pdf www.myjurnal.my/public/article-view.php?id=65095
repository_type Digital Repository
institution_category Local Institution
institution MyJournal
building MyJournal Repository
collection Online Access
topic Life sciences
spellingShingle Life sciences
Tuan Norhafizah binti Tuan Zakaria
Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3? (Ser-9) in liver
description Gynura procumbens, a medicinal plant locally known as Sambung nyawa, is widely used traditionally in South East Asia as a remedy to alleviate symptoms of various ailments including diabetes mellitus. Previous investigators reported that the ethanol plant extract was hypoglycemic in streptozotocin (STZ)-induced diabetic rats and suggested the blood glucose-reducing effect could be due to G. procumbens mimicking insulin effects. Our present study aimed to reevaluate the hypoglycemic effect(s) of G. procumbens and to ascertain the involvement of glycogen synthase kinase (GSK3), a key component of insulin biosignaling in the plant activity. A cell-based assay showed that the three G. procumbens fractions tested did not exhibit anti-GSK3 activities. Oral administrations of hexane, ethyl acetate, n-butanol fractions of G. procumbens (250 mg/kg b.w.) and Glibenclamide (5 mg/kg b.w.) for 14 days in STZ-induced diabetic rats reduced blood glucose levels by 29.7%, 60.1%, 33.5% and 61.7% (p<0.05), respectively compared to diabetic control. Liver glycogen contents were elevated (p<0.05), following administration of the three fractions of G. procumbens in diabetic rats when analysed after 14 days of treatment. Western blot analysis also showed GSK3? in the liver of G. procumbens fractions-treated and Glibenclamide-treated animals were phosphorylated at Ser-9. The above findings indicated that the hypoglycemic action of G. procumbens fractions resulted in the inactivation of GSK3? in liver of diabetic rats. Since G. procumbens fractions did not exhibit GSK3 inhibitory properties, it is possible that the hypoglycemic action of the fractions observed here could be due to direct or indirect effects on upstream components of the insulin biosignaling pathway.
author2 Chong, Ching June
author_facet Chong, Ching June
Tuan Norhafizah binti Tuan Zakaria
format Journal
author Tuan Norhafizah binti Tuan Zakaria
author_sort Tuan Norhafizah binti Tuan Zakaria
title Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3? (Ser-9) in liver
title_short Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3? (Ser-9) in liver
title_full Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3? (Ser-9) in liver
title_fullStr Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3? (Ser-9) in liver
title_full_unstemmed Hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of GSK3? (Ser-9) in liver
title_sort hypoglycemic effects of gynura procumbens fractions on streptozotocin-induced diabetic rats involved phosphorylation of gsk3? (ser-9) in liver
publisher Sains Malaysiana, Penerbit Universiti Kebangsaan Malaysia
publishDate 2012
url http://www.myjurnal.my/public/article-view.php?id=65095
first_indexed 2018-09-20T09:24:26Z
last_indexed 2018-09-20T09:24:26Z
_version_ 1612225710866300928

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