Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain Aq5-12

In this study, a novel glyphosate-degrading shows the ability to reduce molybdenum to molybdenum blue. The enzyme from this bacterium was partially purified and partially characterized to ascertain whether the Mo-reducing enzyme from this bacterium shows better or lower efficiency in reducing molybd...

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Main Author:	Husna Zayadi
Other Authors:	Jamulidin, S.N.K.
Format:	Journal
Published:	Bioremediation Science and Technology Research, Hibiscus Publisher 2016
Subjects:	Biological sciences
Online Access:	http://www.myjurnal.my/public/article-view.php?id=122459

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spelling	oai:www.myjurnal.my:1224592018-09-20T00:00:00Z Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain Aq5-12 Husna Zayadi Biological sciences In this study, a novel glyphosate-degrading shows the ability to reduce molybdenum to molybdenum blue. The enzyme from this bacterium was partially purified and partially characterized to ascertain whether the Mo-reducing enzyme from this bacterium shows better or lower efficiency in reducing molybdenum compared to other Mo-reducing bacterium that only exhibits a single biotransformation activity. The enzyme was partially purified using ammonium sulphate fractionation. The Vmax for the electron donating substrate or NADH was at 1.905 nmole Mo blue/min while the Km was 6.146 mM. The regression coefficient was 0.98. Comparative assessment with the previously characterized Mo-reducing enzyme from various bacteria showed that the Mo-reducing enzyme from Burkholderia vietnamiensis strain AQ5-12 showed a lower enzyme activity. Bioremediation Science and Technology Research, Hibiscus Publisher Jamulidin, S.N.K. 2016-00-00 Journal application/pdf 122459 www.myjurnal.my/filebank/published_article/710652.pdf www.myjurnal.my/public/article-view.php?id=122459
repository_type	Digital Repository
institution_category	Local Institution
institution	MyJournal
building	MyJournal Repository
collection	Online Access
topic	Biological sciences
spellingShingle	Biological sciences Husna Zayadi Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain Aq5-12
description	In this study, a novel glyphosate-degrading shows the ability to reduce molybdenum to molybdenum blue. The enzyme from this bacterium was partially purified and partially characterized to ascertain whether the Mo-reducing enzyme from this bacterium shows better or lower efficiency in reducing molybdenum compared to other Mo-reducing bacterium that only exhibits a single biotransformation activity. The enzyme was partially purified using ammonium sulphate fractionation. The Vmax for the electron donating substrate or NADH was at 1.905 nmole Mo blue/min while the Km was 6.146 mM. The regression coefficient was 0.98. Comparative assessment with the previously characterized Mo-reducing enzyme from various bacteria showed that the Mo-reducing enzyme from Burkholderia vietnamiensis strain AQ5-12 showed a lower enzyme activity.
author2	Jamulidin, S.N.K.
author_facet	Jamulidin, S.N.K. Husna Zayadi
format	Journal
author	Husna Zayadi
author_sort	Husna Zayadi
title	Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain Aq5-12
title_short	Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain Aq5-12
title_full	Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain Aq5-12
title_fullStr	Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain Aq5-12
title_full_unstemmed	Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain Aq5-12
title_sort	partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading burkholderia vietnamiensis strain aq5-12
publisher	Bioremediation Science and Technology Research, Hibiscus Publisher
publishDate	2016
url	http://www.myjurnal.my/public/article-view.php?id=122459
first_indexed	2018-09-20T17:39:44Z
last_indexed	2018-09-20T17:39:44Z
_version_	1612256264903983104

Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain Aq5-12

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