Desiccation tolerance in phaleria macrocarpa embryonic axes

Phaleria macrocarpa seeds are rapidly killed with desiccation to moisture content (MC) below 20%. Desiccation tolerance of their embryonic axes was studied for storage and germplasm conservation purposes. Embryonic axes were extracted aseptically from fresh seeds obtained from fully ripe fruits in a...

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Main Authors:	Ahmed Asrity, S.M., Tsan, F.Y., Ding, P., Syed Aris, S.R.
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2014
Online Access:	http://journalarticle.ukm.my/7808/ http://journalarticle.ukm.my/7808/ http://journalarticle.ukm.my/7808/1/02_S.M._Ahmed_Asrity.pdf

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spelling	oai:generic.eprints.org:78082016-12-14T06:45:16Z http://journalarticle.ukm.my/7808/ Desiccation tolerance in phaleria macrocarpa embryonic axes Ahmed Asrity, S.M. Tsan, F.Y. Ding, P. Syed Aris, S.R. Phaleria macrocarpa seeds are rapidly killed with desiccation to moisture content (MC) below 20%. Desiccation tolerance of their embryonic axes was studied for storage and germplasm conservation purposes. Embryonic axes were extracted aseptically from fresh seeds obtained from fully ripe fruits in a horizontal laminar air flow cabinet. They were then desiccated under aseptic condition for periods ranging from 0-8 h. For each desiccation treatment, embryonic axes were drawn randomly for the determination of MC according to ISTA, electrolyte leakage and proliferation on Murashige and Skoog (MS) media supplemented with 1 mg/l 6-benzylaminopurine (BAP) and 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The results obtained from this study indicated that the embryonic axes could tolerate dehydration down to 13.6% with desiccation for 8 h while retaining relatively high viability of 76.7%. This was supported by only gradual increment of electrolyte leakage with the desiccated embryonic axes. All non-desiccated embryonic axes with MC of 52.5% were capable to grow into normal plantlets in vitro but dehydration to MC of 36.0% and further down to 13.6% generally resulted in callus formation with up to 16.7% of the embryonic axes while at least 60.0% of the other embryonic axes were still capable to proliferate as normal plantlets in vitro. Universiti Kebangsaan Malaysia 2014-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7808/1/02_S.M._Ahmed_Asrity.pdf Ahmed Asrity, S.M. and Tsan, F.Y. and Ding, P. and Syed Aris, S.R. (2014) Desiccation tolerance in phaleria macrocarpa embryonic axes. Sains Malaysiana, 43 (10). pp. 1471-1475. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Phaleria macrocarpa seeds are rapidly killed with desiccation to moisture content (MC) below 20%. Desiccation tolerance of their embryonic axes was studied for storage and germplasm conservation purposes. Embryonic axes were extracted aseptically from fresh seeds obtained from fully ripe fruits in a horizontal laminar air flow cabinet. They were then desiccated under aseptic condition for periods ranging from 0-8 h. For each desiccation treatment, embryonic axes were drawn randomly for the determination of MC according to ISTA, electrolyte leakage and proliferation on Murashige and Skoog (MS) media supplemented with 1 mg/l 6-benzylaminopurine (BAP) and 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The results obtained from this study indicated that the embryonic axes could tolerate dehydration down to 13.6% with desiccation for 8 h while retaining relatively high viability of 76.7%. This was supported by only gradual increment of electrolyte leakage with the desiccated embryonic axes. All non-desiccated embryonic axes with MC of 52.5% were capable to grow into normal plantlets in vitro but dehydration to MC of 36.0% and further down to 13.6% generally resulted in callus formation with up to 16.7% of the embryonic axes while at least 60.0% of the other embryonic axes were still capable to proliferate as normal plantlets in vitro.
format	Article
author	Ahmed Asrity, S.M. Tsan, F.Y. Ding, P. Syed Aris, S.R.
spellingShingle	Ahmed Asrity, S.M. Tsan, F.Y. Ding, P. Syed Aris, S.R. Desiccation tolerance in phaleria macrocarpa embryonic axes
author_facet	Ahmed Asrity, S.M. Tsan, F.Y. Ding, P. Syed Aris, S.R.
author_sort	Ahmed Asrity, S.M.
title	Desiccation tolerance in phaleria macrocarpa embryonic axes
title_short	Desiccation tolerance in phaleria macrocarpa embryonic axes
title_full	Desiccation tolerance in phaleria macrocarpa embryonic axes
title_fullStr	Desiccation tolerance in phaleria macrocarpa embryonic axes
title_full_unstemmed	Desiccation tolerance in phaleria macrocarpa embryonic axes
title_sort	desiccation tolerance in phaleria macrocarpa embryonic axes
publisher	Universiti Kebangsaan Malaysia
publishDate	2014
url	http://journalarticle.ukm.my/7808/ http://journalarticle.ukm.my/7808/ http://journalarticle.ukm.my/7808/1/02_S.M._Ahmed_Asrity.pdf
first_indexed	2018-09-07T10:06:25Z
last_indexed	2018-09-07T10:06:25Z
_version_	1610942812390424576

Desiccation tolerance in phaleria macrocarpa embryonic axes

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