Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants
Effective microbial metabolic engineering is reliant on efficient gene transfer. Here we present a simple screening strategy that may be deployed to isolate rare, hypertransformable variants. The procedure was used to increase the frequency of transformation of the solvent producing organism Clostri...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2017
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| Online Access: | http://eprints.nottingham.ac.uk/47404/ http://eprints.nottingham.ac.uk/47404/ http://eprints.nottingham.ac.uk/47404/ http://eprints.nottingham.ac.uk/47404/1/Variants%201-s2.0-S1075996417301798-main.pdf |