Numerical investigation of the mechanisms of ultrasound-modulated bioluminescence tomography

Objective: A hybrid imaging technique, Ultrasound Modulated Luminescence Tomography, that uses ultrasound to modulate diffusely propagating light has been shown to improve the spatial resolution of optical images. This paper is to investigate the underlying modulation mechanisms and the feasibility...

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Main Authors:	Zhang, Qimei, Mather, Melissa, Morgan, Stephen P.
Format:	Article
Language:	English
Published:	Institute of Electrical and Electronics Engineers 2015
Online Access:	http://eprints.nottingham.ac.uk/46414/ http://eprints.nottingham.ac.uk/46414/ http://eprints.nottingham.ac.uk/46414/ http://eprints.nottingham.ac.uk/46414/1/Qimei_Bioluminescence_Paper_v12%20final%20submitted.pdf

id	nottingham-46414
recordtype	eprints
spelling	nottingham-464142018-07-02T09:15:28Z http://eprints.nottingham.ac.uk/46414/ Numerical investigation of the mechanisms of ultrasound-modulated bioluminescence tomography Zhang, Qimei Mather, Melissa Morgan, Stephen P. Objective: A hybrid imaging technique, Ultrasound Modulated Luminescence Tomography, that uses ultrasound to modulate diffusely propagating light has been shown to improve the spatial resolution of optical images. This paper is to investigate the underlying modulation mechanisms and the feasibility of applying this technique to improve the spatial resolution of bioluminescence tomography. Methods: Ultrasound Modulated Bioluminescence Tomography was studied numerically to identify the dominance of four factors (reduced optical scattering coefficient, optical absorption coefficient, refractive index, and luciferase concentration) on the depth of light modulation. In practice, an open source finite element method tool for simulation of diffusely propagating light, Near Infrared Fluorescence and Spectral Tomography, was modified to incorporate the effects of ultrasound modulation. The signal-to-noise ratios of detected modulated bioluminescent emissions are calculated using the optical and physical properties of a mouse model. Results: The modulation depth of the bioluminescent emission affected by the US induced variation of local concentration of the light emitting enzyme luciferase were at least two orders of magnitude greater than that caused by variations in the other factors. For surface radiances above approximately 107 photons/s/cm2/sr the corresponding SNRs are detectable with the current detector technologies. Conclusion: The dominant effect in generation of ultrasound modulated bioluminescence is ultrasound induced variation in luciferase concentration. The SNR analysis results confirm the feasibility of applying Ultrasound Modulated Bioluminescence Tomography in preclinical imaging of mice. Significance: The simulation model developed suggests ultrasound modulated bioluminescence tomography is a potential technique to improve the spatial resolution of bioluminescence tomography. Institute of Electrical and Electronics Engineers 2015-09-01 Article PeerReviewed application/pdf en http://eprints.nottingham.ac.uk/46414/1/Qimei_Bioluminescence_Paper_v12%20final%20submitted.pdf Zhang, Qimei and Mather, Melissa and Morgan, Stephen P. (2015) Numerical investigation of the mechanisms of ultrasound-modulated bioluminescence tomography. IEEE Transactions on Biomedical Engineering, 62 (9). pp. 2135-2143. ISSN 0018-9294 http://ieeexplore.ieee.org/document/7045571/ doi:10.1109/TBME.2015.2405415 doi:10.1109/TBME.2015.2405415
repository_type	Digital Repository
institution_category	Local University
institution	University of Nottingham Malaysia Campus
building	Nottingham Research Data Repository
collection	Online Access
language	English
description	Objective: A hybrid imaging technique, Ultrasound Modulated Luminescence Tomography, that uses ultrasound to modulate diffusely propagating light has been shown to improve the spatial resolution of optical images. This paper is to investigate the underlying modulation mechanisms and the feasibility of applying this technique to improve the spatial resolution of bioluminescence tomography. Methods: Ultrasound Modulated Bioluminescence Tomography was studied numerically to identify the dominance of four factors (reduced optical scattering coefficient, optical absorption coefficient, refractive index, and luciferase concentration) on the depth of light modulation. In practice, an open source finite element method tool for simulation of diffusely propagating light, Near Infrared Fluorescence and Spectral Tomography, was modified to incorporate the effects of ultrasound modulation. The signal-to-noise ratios of detected modulated bioluminescent emissions are calculated using the optical and physical properties of a mouse model. Results: The modulation depth of the bioluminescent emission affected by the US induced variation of local concentration of the light emitting enzyme luciferase were at least two orders of magnitude greater than that caused by variations in the other factors. For surface radiances above approximately 107 photons/s/cm2/sr the corresponding SNRs are detectable with the current detector technologies. Conclusion: The dominant effect in generation of ultrasound modulated bioluminescence is ultrasound induced variation in luciferase concentration. The SNR analysis results confirm the feasibility of applying Ultrasound Modulated Bioluminescence Tomography in preclinical imaging of mice. Significance: The simulation model developed suggests ultrasound modulated bioluminescence tomography is a potential technique to improve the spatial resolution of bioluminescence tomography.
format	Article
author	Zhang, Qimei Mather, Melissa Morgan, Stephen P.
spellingShingle	Zhang, Qimei Mather, Melissa Morgan, Stephen P. Numerical investigation of the mechanisms of ultrasound-modulated bioluminescence tomography
author_facet	Zhang, Qimei Mather, Melissa Morgan, Stephen P.
author_sort	Zhang, Qimei
title	Numerical investigation of the mechanisms of ultrasound-modulated bioluminescence tomography
title_short	Numerical investigation of the mechanisms of ultrasound-modulated bioluminescence tomography
title_full	Numerical investigation of the mechanisms of ultrasound-modulated bioluminescence tomography
title_fullStr	Numerical investigation of the mechanisms of ultrasound-modulated bioluminescence tomography
title_full_unstemmed	Numerical investigation of the mechanisms of ultrasound-modulated bioluminescence tomography
title_sort	numerical investigation of the mechanisms of ultrasound-modulated bioluminescence tomography
publisher	Institute of Electrical and Electronics Engineers
publishDate	2015
url	http://eprints.nottingham.ac.uk/46414/ http://eprints.nottingham.ac.uk/46414/ http://eprints.nottingham.ac.uk/46414/ http://eprints.nottingham.ac.uk/46414/1/Qimei_Bioluminescence_Paper_v12%20final%20submitted.pdf
first_indexed	2018-09-06T13:46:31Z
last_indexed	2018-09-06T13:46:31Z
_version_	1610866063235350528

Numerical investigation of the mechanisms of ultrasound-modulated bioluminescence tomography

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