Extracellular matrix-derived hydrogels for dental stem cell delivery

Decellularised mammalian extracellular matrices (ECM) have been widely accepted as an ideal substrate for repair and remodelling of numerous tissues in clinical and pre-clinical studies. Recent studies have demonstrated the ability of ECM scaffolds derived from site-specific homologous tissues to di...

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Main Authors:	Viswanath, Aiswarya, Vanacker, Julie, Germain, Loic, Leprince, Julien G., Diogenes, Anibal, Shakesheff, Kevin M., White, Lisa J., des Rieux, Anne
Format:	Article
Language:	English
Published:	Wiley 2016
Online Access:	http://eprints.nottingham.ac.uk/46382/ http://eprints.nottingham.ac.uk/46382/ http://eprints.nottingham.ac.uk/46382/ http://eprints.nottingham.ac.uk/46382/1/White_ECMh%20Dental%20stem%20cells_eprint.pdf

id	nottingham-46382
recordtype	eprints
spelling	nottingham-463822018-06-26T12:41:59Z http://eprints.nottingham.ac.uk/46382/ Extracellular matrix-derived hydrogels for dental stem cell delivery Viswanath, Aiswarya Vanacker, Julie Germain, Loic Leprince, Julien G. Diogenes, Anibal Shakesheff, Kevin M. White, Lisa J. des Rieux, Anne Decellularised mammalian extracellular matrices (ECM) have been widely accepted as an ideal substrate for repair and remodelling of numerous tissues in clinical and pre-clinical studies. Recent studies have demonstrated the ability of ECM scaffolds derived from site-specific homologous tissues to direct cell differentiation. The present study investigated the suitability of hydrogels derived from different source tissues: bone, spinal cord and dentine, as suitable carriers to deliver human apical papilla derived mesenchymal stem cells (SCAP) for spinal cord regeneration. Bone, spinal cord and dentine ECM hydrogels exhibited distinct structural, mechanical and biological characteristics. All three hydrogels supported SCAP viability and proliferation. However, only spinal cord and bone derived hydrogels promoted the expression of neural lineage markers. The specific environment of ECM scaffolds significantly affected the differentiation of SCAP to a neural lineage, with stronger responses observed with spinal cord ECM hydrogels, suggesting that site-specific tissues are more likely to facilitate optimal stem cell behaviour for constructive spinal cord regeneration Wiley 2016-11-05 Article PeerReviewed application/pdf en http://eprints.nottingham.ac.uk/46382/1/White_ECMh%20Dental%20stem%20cells_eprint.pdf Viswanath, Aiswarya and Vanacker, Julie and Germain, Loic and Leprince, Julien G. and Diogenes, Anibal and Shakesheff, Kevin M. and White, Lisa J. and des Rieux, Anne (2016) Extracellular matrix-derived hydrogels for dental stem cell delivery. Journal of Biomedical Materials Research Part A, 105 (1). pp. 319-328. ISSN 1552-4965 http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.35901/abstract;jsessionid=F19EBF527F73CE2DCC30A5CE2328E855.f02t01 doi:10.1002/jbm.a.35901 doi:10.1002/jbm.a.35901
repository_type	Digital Repository
institution_category	Local University
institution	University of Nottingham Malaysia Campus
building	Nottingham Research Data Repository
collection	Online Access
language	English
description	Decellularised mammalian extracellular matrices (ECM) have been widely accepted as an ideal substrate for repair and remodelling of numerous tissues in clinical and pre-clinical studies. Recent studies have demonstrated the ability of ECM scaffolds derived from site-specific homologous tissues to direct cell differentiation. The present study investigated the suitability of hydrogels derived from different source tissues: bone, spinal cord and dentine, as suitable carriers to deliver human apical papilla derived mesenchymal stem cells (SCAP) for spinal cord regeneration. Bone, spinal cord and dentine ECM hydrogels exhibited distinct structural, mechanical and biological characteristics. All three hydrogels supported SCAP viability and proliferation. However, only spinal cord and bone derived hydrogels promoted the expression of neural lineage markers. The specific environment of ECM scaffolds significantly affected the differentiation of SCAP to a neural lineage, with stronger responses observed with spinal cord ECM hydrogels, suggesting that site-specific tissues are more likely to facilitate optimal stem cell behaviour for constructive spinal cord regeneration
format	Article
author	Viswanath, Aiswarya Vanacker, Julie Germain, Loic Leprince, Julien G. Diogenes, Anibal Shakesheff, Kevin M. White, Lisa J. des Rieux, Anne
spellingShingle	Viswanath, Aiswarya Vanacker, Julie Germain, Loic Leprince, Julien G. Diogenes, Anibal Shakesheff, Kevin M. White, Lisa J. des Rieux, Anne Extracellular matrix-derived hydrogels for dental stem cell delivery
author_facet	Viswanath, Aiswarya Vanacker, Julie Germain, Loic Leprince, Julien G. Diogenes, Anibal Shakesheff, Kevin M. White, Lisa J. des Rieux, Anne
author_sort	Viswanath, Aiswarya
title	Extracellular matrix-derived hydrogels for dental stem cell delivery
title_short	Extracellular matrix-derived hydrogels for dental stem cell delivery
title_full	Extracellular matrix-derived hydrogels for dental stem cell delivery
title_fullStr	Extracellular matrix-derived hydrogels for dental stem cell delivery
title_full_unstemmed	Extracellular matrix-derived hydrogels for dental stem cell delivery
title_sort	extracellular matrix-derived hydrogels for dental stem cell delivery
publisher	Wiley
publishDate	2016
url	http://eprints.nottingham.ac.uk/46382/ http://eprints.nottingham.ac.uk/46382/ http://eprints.nottingham.ac.uk/46382/ http://eprints.nottingham.ac.uk/46382/1/White_ECMh%20Dental%20stem%20cells_eprint.pdf
first_indexed	2018-09-06T13:46:19Z
last_indexed	2018-09-06T13:46:19Z
_version_	1610866050242445312

Extracellular matrix-derived hydrogels for dental stem cell delivery

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