Roots-eye view: using microdialysis and microCT to non-destructively map root nutrient depletion and accumulation zones

Improvement in fertiliser use efficiency is a key aspect for achieving sustainable agriculture in order to minimise costs, greenhouse gas emissions and pollution from nutrient runoff. To optimise root architecture for nutrient uptake and efficiency we need to understand what the roots encounter in t...

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Main Authors:	Brackin, Richard, Atkinson, Brian S., Sturrock, Craig J., Rasmussen, Amanda
Format:	Article
Language:	English
Published:	Wiley 2017
Online Access:	http://eprints.nottingham.ac.uk/45450/ http://eprints.nottingham.ac.uk/45450/ http://eprints.nottingham.ac.uk/45450/ http://eprints.nottingham.ac.uk/45450/8/pce13072.pdf

id	nottingham-45450
recordtype	eprints
spelling	nottingham-454502018-07-02T09:15:08Z http://eprints.nottingham.ac.uk/45450/ Roots-eye view: using microdialysis and microCT to non-destructively map root nutrient depletion and accumulation zones Brackin, Richard Atkinson, Brian S. Sturrock, Craig J. Rasmussen, Amanda Improvement in fertiliser use efficiency is a key aspect for achieving sustainable agriculture in order to minimise costs, greenhouse gas emissions and pollution from nutrient runoff. To optimise root architecture for nutrient uptake and efficiency we need to understand what the roots encounter in their environment. Traditional methods of nutrient sampling such as salt extractions can only be done at the end of an experiment, are impractical for sampling locations precisely and give total nutrient values which can overestimate the nutrients available to the roots. In contrast, microdialysis provides a non-invasive, continuous method for sampling available nutrients in the soil. Here for the first time we have used microCT imaging to position microdialysis probes at known distances from the roots and then measured the available nitrate and ammonium. We found that nitrate accumulated close to roots while ammonium was depleted demonstrating that this combination of complementary techniques provides a unique ability to measure root-available nutrients non-destructively and in almost real-time. Wiley 2017-12 Article PeerReviewed application/pdf en cc_by http://eprints.nottingham.ac.uk/45450/8/pce13072.pdf Brackin, Richard and Atkinson, Brian S. and Sturrock, Craig J. and Rasmussen, Amanda (2017) Roots-eye view: using microdialysis and microCT to non-destructively map root nutrient depletion and accumulation zones. Plant, Cell and Environment, 40 (12). pp. 3135-3142. ISSN 1365-3040 http://onlinelibrary.wiley.com/doi/10.1111/pce.13072/abstract doi:10.1111/pce.13072 doi:10.1111/pce.13072
repository_type	Digital Repository
institution_category	Local University
institution	University of Nottingham Malaysia Campus
building	Nottingham Research Data Repository
collection	Online Access
language	English
description	Improvement in fertiliser use efficiency is a key aspect for achieving sustainable agriculture in order to minimise costs, greenhouse gas emissions and pollution from nutrient runoff. To optimise root architecture for nutrient uptake and efficiency we need to understand what the roots encounter in their environment. Traditional methods of nutrient sampling such as salt extractions can only be done at the end of an experiment, are impractical for sampling locations precisely and give total nutrient values which can overestimate the nutrients available to the roots. In contrast, microdialysis provides a non-invasive, continuous method for sampling available nutrients in the soil. Here for the first time we have used microCT imaging to position microdialysis probes at known distances from the roots and then measured the available nitrate and ammonium. We found that nitrate accumulated close to roots while ammonium was depleted demonstrating that this combination of complementary techniques provides a unique ability to measure root-available nutrients non-destructively and in almost real-time.
format	Article
author	Brackin, Richard Atkinson, Brian S. Sturrock, Craig J. Rasmussen, Amanda
spellingShingle	Brackin, Richard Atkinson, Brian S. Sturrock, Craig J. Rasmussen, Amanda Roots-eye view: using microdialysis and microCT to non-destructively map root nutrient depletion and accumulation zones
author_facet	Brackin, Richard Atkinson, Brian S. Sturrock, Craig J. Rasmussen, Amanda
author_sort	Brackin, Richard
title	Roots-eye view: using microdialysis and microCT to non-destructively map root nutrient depletion and accumulation zones
title_short	Roots-eye view: using microdialysis and microCT to non-destructively map root nutrient depletion and accumulation zones
title_full	Roots-eye view: using microdialysis and microCT to non-destructively map root nutrient depletion and accumulation zones
title_fullStr	Roots-eye view: using microdialysis and microCT to non-destructively map root nutrient depletion and accumulation zones
title_full_unstemmed	Roots-eye view: using microdialysis and microCT to non-destructively map root nutrient depletion and accumulation zones
title_sort	roots-eye view: using microdialysis and microct to non-destructively map root nutrient depletion and accumulation zones
publisher	Wiley
publishDate	2017
url	http://eprints.nottingham.ac.uk/45450/ http://eprints.nottingham.ac.uk/45450/ http://eprints.nottingham.ac.uk/45450/ http://eprints.nottingham.ac.uk/45450/8/pce13072.pdf
first_indexed	2018-09-06T13:40:32Z
last_indexed	2018-09-06T13:40:32Z
_version_	1610865687238017024

Roots-eye view: using microdialysis and microCT to non-destructively map root nutrient depletion and accumulation zones

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