Unique characterisation of Langerhans cells in the spleen of the African catfish (Clarias gariepinus)

The African catfish (Clarias gariepinus) has increasingly become an economically important food source especially in Africa, Asia and Europe, with exports extending to countries throughout the world. In the last 25 years, aquaculture of this species has been particularly effective with large increas...

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Main Authors:	Zaghloul, Doaa M., Derbalah, Amira, Rutland, Catrin S.
Format:	Article
Language:	English
Published:	ScienceMatters 2017
Online Access:	http://eprints.nottingham.ac.uk/44420/ http://eprints.nottingham.ac.uk/44420/ http://eprints.nottingham.ac.uk/44420/ http://eprints.nottingham.ac.uk/44420/1/Unique%20characterization%20of%20Langerhans%20cells%20in%20the%20spleen%20of%20the%20African%20catfish%20%28_em_Clarias%20gariepinus__em_%29.pdf

id	nottingham-44420
recordtype	eprints
spelling	nottingham-444202017-10-12T23:32:36Z http://eprints.nottingham.ac.uk/44420/ Unique characterisation of Langerhans cells in the spleen of the African catfish (Clarias gariepinus) Zaghloul, Doaa M. Derbalah, Amira Rutland, Catrin S. The African catfish (Clarias gariepinus) has increasingly become an economically important food source especially in Africa, Asia and Europe, with exports extending to countries throughout the world. In the last 25 years, aquaculture of this species has been particularly effective with large increases in production and this is in part due to the catfish being highly resistant to disease. In order to further understand the immune system of the catfish, the spleen was investigated using a number of staining, immunohistochemical, light and transmission electron microscope techniques in order to verify the histological features of this organ. The gross histological features of the spleen confirmed the presence of a capsule containing numerous collagen fibers surrounded the spleen of the African catfish. Melanomacrophage centers, lymphocytes and surrounding arterial vessels were the main components of the white pulp whilst the red pulp contained sinusoids, capillaries and splenic cords of erythrocytes, macrophages and lymphocytes. Transmission electron microscopy identified Langerhans-like cells which were irregular to ovoid in shape with an electron lucent cytoplasm. The cells were characterized by the presence of numerous Birbeck-like granules, varying in shape from oval, round, rod to racket shaped granules. In total, three types of Birbeck-like granules were identified. The first type had heterogenic content, the second type were cored granules and the third type were racket shaped granules. Some of the racket shaped granules were enlarged and contained granular content. The Langerhans-like cells were further identified and characterised by showing a positive reaction with antibodies to S100 and CD1a. The existence of Langerhans-like cells within the spleen helps to identify the components of the immune system within the African catfish. ScienceMatters 2017-07-21 Article PeerReviewed application/pdf en cc_by http://eprints.nottingham.ac.uk/44420/1/Unique%20characterization%20of%20Langerhans%20cells%20in%20the%20spleen%20of%20the%20African%20catfish%20%28_em_Clarias%20gariepinus__em_%29.pdf Zaghloul, Doaa M. and Derbalah, Amira and Rutland, Catrin S. (2017) Unique characterisation of Langerhans cells in the spleen of the African catfish (Clarias gariepinus). Matters . pp. 1-14. https://www.sciencematters.io/articles/201703000005/info doi:10.19185/matters.201703000005 doi:10.19185/matters.201703000005
repository_type	Digital Repository
institution_category	Local University
institution	University of Nottingham Malaysia Campus
building	Nottingham Research Data Repository
collection	Online Access
language	English
description	The African catfish (Clarias gariepinus) has increasingly become an economically important food source especially in Africa, Asia and Europe, with exports extending to countries throughout the world. In the last 25 years, aquaculture of this species has been particularly effective with large increases in production and this is in part due to the catfish being highly resistant to disease. In order to further understand the immune system of the catfish, the spleen was investigated using a number of staining, immunohistochemical, light and transmission electron microscope techniques in order to verify the histological features of this organ. The gross histological features of the spleen confirmed the presence of a capsule containing numerous collagen fibers surrounded the spleen of the African catfish. Melanomacrophage centers, lymphocytes and surrounding arterial vessels were the main components of the white pulp whilst the red pulp contained sinusoids, capillaries and splenic cords of erythrocytes, macrophages and lymphocytes. Transmission electron microscopy identified Langerhans-like cells which were irregular to ovoid in shape with an electron lucent cytoplasm. The cells were characterized by the presence of numerous Birbeck-like granules, varying in shape from oval, round, rod to racket shaped granules. In total, three types of Birbeck-like granules were identified. The first type had heterogenic content, the second type were cored granules and the third type were racket shaped granules. Some of the racket shaped granules were enlarged and contained granular content. The Langerhans-like cells were further identified and characterised by showing a positive reaction with antibodies to S100 and CD1a. The existence of Langerhans-like cells within the spleen helps to identify the components of the immune system within the African catfish.
format	Article
author	Zaghloul, Doaa M. Derbalah, Amira Rutland, Catrin S.
spellingShingle	Zaghloul, Doaa M. Derbalah, Amira Rutland, Catrin S. Unique characterisation of Langerhans cells in the spleen of the African catfish (Clarias gariepinus)
author_facet	Zaghloul, Doaa M. Derbalah, Amira Rutland, Catrin S.
author_sort	Zaghloul, Doaa M.
title	Unique characterisation of Langerhans cells in the spleen of the African catfish (Clarias gariepinus)
title_short	Unique characterisation of Langerhans cells in the spleen of the African catfish (Clarias gariepinus)
title_full	Unique characterisation of Langerhans cells in the spleen of the African catfish (Clarias gariepinus)
title_fullStr	Unique characterisation of Langerhans cells in the spleen of the African catfish (Clarias gariepinus)
title_full_unstemmed	Unique characterisation of Langerhans cells in the spleen of the African catfish (Clarias gariepinus)
title_sort	unique characterisation of langerhans cells in the spleen of the african catfish (clarias gariepinus)
publisher	ScienceMatters
publishDate	2017
url	http://eprints.nottingham.ac.uk/44420/ http://eprints.nottingham.ac.uk/44420/ http://eprints.nottingham.ac.uk/44420/ http://eprints.nottingham.ac.uk/44420/1/Unique%20characterization%20of%20Langerhans%20cells%20in%20the%20spleen%20of%20the%20African%20catfish%20%28_em_Clarias%20gariepinus__em_%29.pdf
first_indexed	2018-09-06T13:33:29Z
last_indexed	2018-09-06T13:33:29Z
_version_	1610865243279327232

Unique characterisation of Langerhans cells in the spleen of the African catfish (Clarias gariepinus)

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