Bacterial resistance to arsenic protects against protist killing

Protists kill their bacterial prey using toxic metals such as copper. Here we hypothesize that the metalloid arsenic has a similar role. To test this hypothesis, we examined intracellular survival of Escherichia coli (E. coli) in the amoeba Dictyostelium discoideum (D. discoideum). Deletion of the E...

Full description

Bibliographic Details
Main Authors:	Hao, Xiuli, Li, Xuanji, Pal, Chandan, Hobman, Jon L., Larsson, D.G. Joakim, Saquib, Quaiser, Alwathnani, Hend A., Rosen, Barry P., Zhu, Yong-Guan, Rensing, Christopher
Format:	Article
Language:	English
Published:	Wiley 2017
Online Access:	http://eprints.nottingham.ac.uk/43238/ http://eprints.nottingham.ac.uk/43238/ http://eprints.nottingham.ac.uk/43238/ http://eprints.nottingham.ac.uk/43238/8/CR%20Frontier%20Perspective%20copper_Final%20revision%20SFM.pdf

id	nottingham-43238
recordtype	eprints
spelling	nottingham-432382018-02-17T09:08:26Z http://eprints.nottingham.ac.uk/43238/ Bacterial resistance to arsenic protects against protist killing Hao, Xiuli Li, Xuanji Pal, Chandan Hobman, Jon L. Larsson, D.G. Joakim Saquib, Quaiser Alwathnani, Hend A. Rosen, Barry P. Zhu, Yong-Guan Rensing, Christopher Protists kill their bacterial prey using toxic metals such as copper. Here we hypothesize that the metalloid arsenic has a similar role. To test this hypothesis, we examined intracellular survival of Escherichia coli (E. coli) in the amoeba Dictyostelium discoideum (D. discoideum). Deletion of the E. coli ars operon led to significantly lower intracellular survival compared to wild type E. coli. This suggests that protists use arsenic to poison bacterial cells in the phagosome, similar to their use of copper. In response to copper and arsenic poisoning by protists, there is selection for acquisition of arsenic and copper resistance genes in the bacterial prey to avoid killing. In agreement with this hypothesis, both copper and arsenic resistance determinants are widespread in many bacterial taxa and environments, and they are often found together on plasmids. A role for heavy metals and arsenic in the ancient predator–prey relationship between protists and bacteria could explain the widespread presence of metal resistance determinants in pristine environments. Wiley 2017-04 Article PeerReviewed application/pdf en http://eprints.nottingham.ac.uk/43238/8/CR%20Frontier%20Perspective%20copper_Final%20revision%20SFM.pdf Hao, Xiuli and Li, Xuanji and Pal, Chandan and Hobman, Jon L. and Larsson, D.G. Joakim and Saquib, Quaiser and Alwathnani, Hend A. and Rosen, Barry P. and Zhu, Yong-Guan and Rensing, Christopher (2017) Bacterial resistance to arsenic protects against protist killing. BioMetals, 30 (2). pp. 307-311. ISSN 1572-8773 https://link.springer.com/article/10.1007%2Fs10534-017-0003-4 doi:10.1007/s10534-017-0003-4 doi:10.1007/s10534-017-0003-4
repository_type	Digital Repository
institution_category	Local University
institution	University of Nottingham Malaysia Campus
building	Nottingham Research Data Repository
collection	Online Access
language	English
description	Protists kill their bacterial prey using toxic metals such as copper. Here we hypothesize that the metalloid arsenic has a similar role. To test this hypothesis, we examined intracellular survival of Escherichia coli (E. coli) in the amoeba Dictyostelium discoideum (D. discoideum). Deletion of the E. coli ars operon led to significantly lower intracellular survival compared to wild type E. coli. This suggests that protists use arsenic to poison bacterial cells in the phagosome, similar to their use of copper. In response to copper and arsenic poisoning by protists, there is selection for acquisition of arsenic and copper resistance genes in the bacterial prey to avoid killing. In agreement with this hypothesis, both copper and arsenic resistance determinants are widespread in many bacterial taxa and environments, and they are often found together on plasmids. A role for heavy metals and arsenic in the ancient predator–prey relationship between protists and bacteria could explain the widespread presence of metal resistance determinants in pristine environments.
format	Article
author	Hao, Xiuli Li, Xuanji Pal, Chandan Hobman, Jon L. Larsson, D.G. Joakim Saquib, Quaiser Alwathnani, Hend A. Rosen, Barry P. Zhu, Yong-Guan Rensing, Christopher
spellingShingle	Hao, Xiuli Li, Xuanji Pal, Chandan Hobman, Jon L. Larsson, D.G. Joakim Saquib, Quaiser Alwathnani, Hend A. Rosen, Barry P. Zhu, Yong-Guan Rensing, Christopher Bacterial resistance to arsenic protects against protist killing
author_facet	Hao, Xiuli Li, Xuanji Pal, Chandan Hobman, Jon L. Larsson, D.G. Joakim Saquib, Quaiser Alwathnani, Hend A. Rosen, Barry P. Zhu, Yong-Guan Rensing, Christopher
author_sort	Hao, Xiuli
title	Bacterial resistance to arsenic protects against protist killing
title_short	Bacterial resistance to arsenic protects against protist killing
title_full	Bacterial resistance to arsenic protects against protist killing
title_fullStr	Bacterial resistance to arsenic protects against protist killing
title_full_unstemmed	Bacterial resistance to arsenic protects against protist killing
title_sort	bacterial resistance to arsenic protects against protist killing
publisher	Wiley
publishDate	2017
url	http://eprints.nottingham.ac.uk/43238/ http://eprints.nottingham.ac.uk/43238/ http://eprints.nottingham.ac.uk/43238/ http://eprints.nottingham.ac.uk/43238/8/CR%20Frontier%20Perspective%20copper_Final%20revision%20SFM.pdf
first_indexed	2018-09-06T13:24:53Z
last_indexed	2018-09-06T13:24:53Z
_version_	1610864701882761216

Bacterial resistance to arsenic protects against protist killing

Similar Items