Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches

Injectable poly (dl-lactic-co-glycolic acid) (PdlLGA) microspheres are promising candidates as biodegradable controlled release carriers for drug and cell delivery applications; however, they have limited functional groups on the surface to enable dense grafting of tissue specific biocompatible mole...

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Main Authors:	Baki, Abdulrahman, Rahman, Cheryl V., White, Lisa J., Scurr, David J., Qutachi, Omar, Shakesheff, Kevin M.
Format:	Article
Language:	English English
Published:	Elsevier 2017
Online Access:	http://eprints.nottingham.ac.uk/41899/ http://eprints.nottingham.ac.uk/41899/ http://eprints.nottingham.ac.uk/41899/ http://eprints.nottingham.ac.uk/41899/1/1-s2.0-S174270611730051X-main.pdf http://eprints.nottingham.ac.uk/41899/8/Baki-Surface%20Modification%20of%20PDLLGA%20Microspher%20%28002%29.pdf

id	nottingham-41899
recordtype	eprints
spelling	nottingham-418992018-06-12T05:44:15Z http://eprints.nottingham.ac.uk/41899/ Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches Baki, Abdulrahman Rahman, Cheryl V. White, Lisa J. Scurr, David J. Qutachi, Omar Shakesheff, Kevin M. Injectable poly (dl-lactic-co-glycolic acid) (PdlLGA) microspheres are promising candidates as biodegradable controlled release carriers for drug and cell delivery applications; however, they have limited functional groups on the surface to enable dense grafting of tissue specific biocompatible molecules. In this study we have evaluated surface adsorption, entrapment and oxygen plasma treatment as three approaches to modify the surfaces of PdlLGA microspheres with gelatine methacrylate (gel-MA) as a biocompatible and photo cross-linkable macromolecule. Time of flight secondary ion mass spectroscopy (TOF SIMS) and X-ray photoelectron spectroscopy (XPS) were used to detect and quantify gel-MA on the surfaces. Fluorescent and scanning electron microscopies (SEM) were used to image the topographical changes. Human mesenchymal stem cells (hMSCs) of immortalised cell line were cultured on the surface of gel-MA modified PdlLGA microspheres and Presto-Blue assay was used to study the effect of different surface modifications on cell proliferation. Data analysis showed that the oxygen plasma treatment approach resulted in the highest density of gel-MA deposition. This study supports oxygen plasma treatment as a facile approach to modify the surface of injectable PdlLGA microspheres with macromolecules such as gel-MA to enhance proliferation rate of injected cells and potentially enable further grafting of tissue specific molecules. Elsevier 2017-01-16 Article PeerReviewed application/pdf en cc_by http://eprints.nottingham.ac.uk/41899/1/1-s2.0-S174270611730051X-main.pdf application/pdf en http://eprints.nottingham.ac.uk/41899/8/Baki-Surface%20Modification%20of%20PDLLGA%20Microspher%20%28002%29.pdf Baki, Abdulrahman and Rahman, Cheryl V. and White, Lisa J. and Scurr, David J. and Qutachi, Omar and Shakesheff, Kevin M. (2017) Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches. Acta Biomaterialia . ISSN 1878-7568 https://doi.org/10.1016/j.actbio.2017.01.042 doi:10.1016/j.actbio.2017.01.042 doi:10.1016/j.actbio.2017.01.042
repository_type	Digital Repository
institution_category	Local University
institution	University of Nottingham Malaysia Campus
building	Nottingham Research Data Repository
collection	Online Access
language	English English
description	Injectable poly (dl-lactic-co-glycolic acid) (PdlLGA) microspheres are promising candidates as biodegradable controlled release carriers for drug and cell delivery applications; however, they have limited functional groups on the surface to enable dense grafting of tissue specific biocompatible molecules. In this study we have evaluated surface adsorption, entrapment and oxygen plasma treatment as three approaches to modify the surfaces of PdlLGA microspheres with gelatine methacrylate (gel-MA) as a biocompatible and photo cross-linkable macromolecule. Time of flight secondary ion mass spectroscopy (TOF SIMS) and X-ray photoelectron spectroscopy (XPS) were used to detect and quantify gel-MA on the surfaces. Fluorescent and scanning electron microscopies (SEM) were used to image the topographical changes. Human mesenchymal stem cells (hMSCs) of immortalised cell line were cultured on the surface of gel-MA modified PdlLGA microspheres and Presto-Blue assay was used to study the effect of different surface modifications on cell proliferation. Data analysis showed that the oxygen plasma treatment approach resulted in the highest density of gel-MA deposition. This study supports oxygen plasma treatment as a facile approach to modify the surface of injectable PdlLGA microspheres with macromolecules such as gel-MA to enhance proliferation rate of injected cells and potentially enable further grafting of tissue specific molecules.
format	Article
author	Baki, Abdulrahman Rahman, Cheryl V. White, Lisa J. Scurr, David J. Qutachi, Omar Shakesheff, Kevin M.
spellingShingle	Baki, Abdulrahman Rahman, Cheryl V. White, Lisa J. Scurr, David J. Qutachi, Omar Shakesheff, Kevin M. Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches
author_facet	Baki, Abdulrahman Rahman, Cheryl V. White, Lisa J. Scurr, David J. Qutachi, Omar Shakesheff, Kevin M.
author_sort	Baki, Abdulrahman
title	Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches
title_short	Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches
title_full	Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches
title_fullStr	Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches
title_full_unstemmed	Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches
title_sort	surface modification of pdllga microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches
publisher	Elsevier
publishDate	2017
url	http://eprints.nottingham.ac.uk/41899/ http://eprints.nottingham.ac.uk/41899/ http://eprints.nottingham.ac.uk/41899/ http://eprints.nottingham.ac.uk/41899/1/1-s2.0-S174270611730051X-main.pdf http://eprints.nottingham.ac.uk/41899/8/Baki-Surface%20Modification%20of%20PDLLGA%20Microspher%20%28002%29.pdf
first_indexed	2018-09-06T13:16:25Z
last_indexed	2018-09-06T13:16:25Z
_version_	1610864169582592000

Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches

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