High-throughput phenotyping (HTP) identifies seedling root traits linked to variation in seed yield and nutrient capture in field-grown oilseed rape (Brassica napus L.)

Background and Aims. Root traits can be selected for crop improvement. Techniques such as soil excavations can be used to screen root traits in the field, but are limited to genotypes that are well-adapted to field conditions. The aim of this study was to compare a low-cost, high-throughput root phe...

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Main Authors:	Thomas, Catherine L., Graham, Neil .S., Hayden, Rory, Meacham, Mark C., Neugebauer, Konrad, Nightingale, Mark, Dupuy, Lionel X., Hammond, John P., White, Philip J., Broadley, Martin R.
Format:	Article
Language:	English
Published:	Oxford Journals 2016
Online Access:	http://eprints.nottingham.ac.uk/37691/ http://eprints.nottingham.ac.uk/37691/ http://eprints.nottingham.ac.uk/37691/ http://eprints.nottingham.ac.uk/37691/1/Ann%20Bot-2016-Thomas-655-65.pdf

id	nottingham-37691
recordtype	eprints
spelling	nottingham-376912018-08-31T16:42:08Z http://eprints.nottingham.ac.uk/37691/ High-throughput phenotyping (HTP) identifies seedling root traits linked to variation in seed yield and nutrient capture in field-grown oilseed rape (Brassica napus L.) Thomas, Catherine L. Graham, Neil .S. Hayden, Rory Meacham, Mark C. Neugebauer, Konrad Nightingale, Mark Dupuy, Lionel X. Hammond, John P. White, Philip J. Broadley, Martin R. Background and Aims. Root traits can be selected for crop improvement. Techniques such as soil excavations can be used to screen root traits in the field, but are limited to genotypes that are well-adapted to field conditions. The aim of this study was to compare a low-cost, high-throughput root phenotyping (HTP) technique in a controlled environment with field performance, using oilseed rape (OSR; Brassica napus) varieties. Methods. Primary root length (PRL), lateral root length and lateral root density (LRD) were measured on 14-d- old seedlings of elite OSR varieties (n 1⁄4 32) using a ‘pouch and wick’ HTP system (~40 replicates). Six field exper- iments were conducted using the same varieties at two UK sites each year for 3 years. Plants were excavated at the 6- to 8-leaf stage for general vigour assessments of roots and shoots in all six experiments, and final seed yield was determined. Leaves were sampled for mineral composition from one of the field experiments. Key Results. Seedling PRL in the HTP system correlated with seed yield in four out of six (r =0.50, 0.50, 0.33, 0.49; P < 0.05) and with emergence in three out of five (r= 0.59, 0.22, 0.49; P < 0.05) field experiments. Seedling LRD correlated positively with leaf concentrations of some minerals, e.g. calcium (r = 0.46; P < 0.01) and zinc (r = 0.58; P < 0.001), but did not correlate with emergence, general early vigour or yield in the field. Conclusions. Associations between PRL and field performance are generally related to early vigour. These root traits might therefore be of limited additional selection value, given that vigour can be measured easily on shoots/ canopies. In contrast, LRD cannot be assessed easily in the field and, if LRD can improve nutrient uptake, then it may be possible to use HTP systems to screen this trait in both elite and more genetically diverse, non-field-adapted OSR. Oxford Journals 2016-04-06 Article PeerReviewed application/pdf en cc_by http://eprints.nottingham.ac.uk/37691/1/Ann%20Bot-2016-Thomas-655-65.pdf Thomas, Catherine L. and Graham, Neil .S. and Hayden, Rory and Meacham, Mark C. and Neugebauer, Konrad and Nightingale, Mark and Dupuy, Lionel X. and Hammond, John P. and White, Philip J. and Broadley, Martin R. (2016) High-throughput phenotyping (HTP) identifies seedling root traits linked to variation in seed yield and nutrient capture in field-grown oilseed rape (Brassica napus L.). Annals of Botany, 118 (4). pp. 655-665. ISSN 1095-8290 http://aob.oxfordjournals.org/content/118/4/655 doi:10.1093/aob/mcw046 doi:10.1093/aob/mcw046
repository_type	Digital Repository
institution_category	Local University
institution	University of Nottingham Malaysia Campus
building	Nottingham Research Data Repository
collection	Online Access
language	English
description	Background and Aims. Root traits can be selected for crop improvement. Techniques such as soil excavations can be used to screen root traits in the field, but are limited to genotypes that are well-adapted to field conditions. The aim of this study was to compare a low-cost, high-throughput root phenotyping (HTP) technique in a controlled environment with field performance, using oilseed rape (OSR; Brassica napus) varieties. Methods. Primary root length (PRL), lateral root length and lateral root density (LRD) were measured on 14-d- old seedlings of elite OSR varieties (n 1⁄4 32) using a ‘pouch and wick’ HTP system (~40 replicates). Six field exper- iments were conducted using the same varieties at two UK sites each year for 3 years. Plants were excavated at the 6- to 8-leaf stage for general vigour assessments of roots and shoots in all six experiments, and final seed yield was determined. Leaves were sampled for mineral composition from one of the field experiments. Key Results. Seedling PRL in the HTP system correlated with seed yield in four out of six (r =0.50, 0.50, 0.33, 0.49; P < 0.05) and with emergence in three out of five (r= 0.59, 0.22, 0.49; P < 0.05) field experiments. Seedling LRD correlated positively with leaf concentrations of some minerals, e.g. calcium (r = 0.46; P < 0.01) and zinc (r = 0.58; P < 0.001), but did not correlate with emergence, general early vigour or yield in the field. Conclusions. Associations between PRL and field performance are generally related to early vigour. These root traits might therefore be of limited additional selection value, given that vigour can be measured easily on shoots/ canopies. In contrast, LRD cannot be assessed easily in the field and, if LRD can improve nutrient uptake, then it may be possible to use HTP systems to screen this trait in both elite and more genetically diverse, non-field-adapted OSR.
format	Article
author	Thomas, Catherine L. Graham, Neil .S. Hayden, Rory Meacham, Mark C. Neugebauer, Konrad Nightingale, Mark Dupuy, Lionel X. Hammond, John P. White, Philip J. Broadley, Martin R.
spellingShingle	Thomas, Catherine L. Graham, Neil .S. Hayden, Rory Meacham, Mark C. Neugebauer, Konrad Nightingale, Mark Dupuy, Lionel X. Hammond, John P. White, Philip J. Broadley, Martin R. High-throughput phenotyping (HTP) identifies seedling root traits linked to variation in seed yield and nutrient capture in field-grown oilseed rape (Brassica napus L.)
author_facet	Thomas, Catherine L. Graham, Neil .S. Hayden, Rory Meacham, Mark C. Neugebauer, Konrad Nightingale, Mark Dupuy, Lionel X. Hammond, John P. White, Philip J. Broadley, Martin R.
author_sort	Thomas, Catherine L.
title	High-throughput phenotyping (HTP) identifies seedling root traits linked to variation in seed yield and nutrient capture in field-grown oilseed rape (Brassica napus L.)
title_short	High-throughput phenotyping (HTP) identifies seedling root traits linked to variation in seed yield and nutrient capture in field-grown oilseed rape (Brassica napus L.)
title_full	High-throughput phenotyping (HTP) identifies seedling root traits linked to variation in seed yield and nutrient capture in field-grown oilseed rape (Brassica napus L.)
title_fullStr	High-throughput phenotyping (HTP) identifies seedling root traits linked to variation in seed yield and nutrient capture in field-grown oilseed rape (Brassica napus L.)
title_full_unstemmed	High-throughput phenotyping (HTP) identifies seedling root traits linked to variation in seed yield and nutrient capture in field-grown oilseed rape (Brassica napus L.)
title_sort	high-throughput phenotyping (htp) identifies seedling root traits linked to variation in seed yield and nutrient capture in field-grown oilseed rape (brassica napus l.)
publisher	Oxford Journals
publishDate	2016
url	http://eprints.nottingham.ac.uk/37691/ http://eprints.nottingham.ac.uk/37691/ http://eprints.nottingham.ac.uk/37691/ http://eprints.nottingham.ac.uk/37691/1/Ann%20Bot-2016-Thomas-655-65.pdf
first_indexed	2018-09-06T12:48:53Z
last_indexed	2018-09-06T12:48:53Z
_version_	1610862437022564352

High-throughput phenotyping (HTP) identifies seedling root traits linked to variation in seed yield and nutrient capture in field-grown oilseed rape (Brassica napus L.)

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