Multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from a dairy farm

Escherichia coli strains were isolated from a single dairy farm as a sentinel organism for the persistence of antibiotic resistance genes in the farm environment. Selective microbiological media were used to isolate 126 E. coli isolates from slurry and faeces samples from different farm areas. Antib...

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Main Authors:	Ibrahim, Delveen, Dodd, Christine E.R., Stekel, Dov J., Ramsden, S.J., Hobman, Jon L.
Format:	Article
Language:	English
Published:	Oxford University Press 2016
Online Access:	http://eprints.nottingham.ac.uk/31623/ http://eprints.nottingham.ac.uk/31623/ http://eprints.nottingham.ac.uk/31623/ http://eprints.nottingham.ac.uk/31623/1/Ibrahim%20et%20al%202016.pdf

id	nottingham-31623
recordtype	eprints
spelling	nottingham-316232017-10-13T22:47:55Z http://eprints.nottingham.ac.uk/31623/ Multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from a dairy farm Ibrahim, Delveen Dodd, Christine E.R. Stekel, Dov J. Ramsden, S.J. Hobman, Jon L. Escherichia coli strains were isolated from a single dairy farm as a sentinel organism for the persistence of antibiotic resistance genes in the farm environment. Selective microbiological media were used to isolate 126 E. coli isolates from slurry and faeces samples from different farm areas. Antibiotic resistance profiling for 17 antibiotics (seven antibiotic classes), showed 57.9% of the isolates were resistant to between 3 and 15 antibiotics. The highest frequency of resistance was to ampicillin (56.3%), and the lowest to imipenem (1.6%), which appeared to be an unstable phenotype and was subsequently lost. Extended spectrum beta-lactamase resistance (ESBL) was detected in 53 isolates and blaCTX-M, blaTEM and blaOXA genes were detected by PCR in twelve, four and two strains, respectively. Phenotypically most isolates showing resistance to cephalosporins were AmpC rather than ESBL, a number of isolates having both activities. Phenotypic resistance patterns suggested co-acquisition of some resistance genes within subsets of the isolates. Genotyping using ERIC PCR demonstrated these were not clonal, and therefore co-resistance may be associated with mobile genetic elements. These data show a snapshot of diverse resistance genes present in the E. coli population reservoir, including resistance to historically used antibiotics as well as cephalosporins in contemporary use. Oxford University Press 2016-04-01 Article PeerReviewed application/pdf en http://eprints.nottingham.ac.uk/31623/1/Ibrahim%20et%20al%202016.pdf Ibrahim, Delveen and Dodd, Christine E.R. and Stekel, Dov J. and Ramsden, S.J. and Hobman, Jon L. (2016) Multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from a dairy farm. FEMS Microbiology Ecology, 92 (4). pp. 1-37. ISSN 1574-6941 http://femsec.oxfordjournals.org/content/early/2016/02/04/femsec.fiw013.long doi:10.1093/femsec/fiw013 doi:10.1093/femsec/fiw013
repository_type	Digital Repository
institution_category	Local University
institution	University of Nottingham Malaysia Campus
building	Nottingham Research Data Repository
collection	Online Access
language	English
description	Escherichia coli strains were isolated from a single dairy farm as a sentinel organism for the persistence of antibiotic resistance genes in the farm environment. Selective microbiological media were used to isolate 126 E. coli isolates from slurry and faeces samples from different farm areas. Antibiotic resistance profiling for 17 antibiotics (seven antibiotic classes), showed 57.9% of the isolates were resistant to between 3 and 15 antibiotics. The highest frequency of resistance was to ampicillin (56.3%), and the lowest to imipenem (1.6%), which appeared to be an unstable phenotype and was subsequently lost. Extended spectrum beta-lactamase resistance (ESBL) was detected in 53 isolates and blaCTX-M, blaTEM and blaOXA genes were detected by PCR in twelve, four and two strains, respectively. Phenotypically most isolates showing resistance to cephalosporins were AmpC rather than ESBL, a number of isolates having both activities. Phenotypic resistance patterns suggested co-acquisition of some resistance genes within subsets of the isolates. Genotyping using ERIC PCR demonstrated these were not clonal, and therefore co-resistance may be associated with mobile genetic elements. These data show a snapshot of diverse resistance genes present in the E. coli population reservoir, including resistance to historically used antibiotics as well as cephalosporins in contemporary use.
format	Article
author	Ibrahim, Delveen Dodd, Christine E.R. Stekel, Dov J. Ramsden, S.J. Hobman, Jon L.
spellingShingle	Ibrahim, Delveen Dodd, Christine E.R. Stekel, Dov J. Ramsden, S.J. Hobman, Jon L. Multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from a dairy farm
author_facet	Ibrahim, Delveen Dodd, Christine E.R. Stekel, Dov J. Ramsden, S.J. Hobman, Jon L.
author_sort	Ibrahim, Delveen
title	Multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from a dairy farm
title_short	Multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from a dairy farm
title_full	Multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from a dairy farm
title_fullStr	Multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from a dairy farm
title_full_unstemmed	Multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from a dairy farm
title_sort	multidrug resistant, extended spectrum β-lactamase (esbl)-producing escherichia coli isolated from a dairy farm
publisher	Oxford University Press
publishDate	2016
url	http://eprints.nottingham.ac.uk/31623/ http://eprints.nottingham.ac.uk/31623/ http://eprints.nottingham.ac.uk/31623/ http://eprints.nottingham.ac.uk/31623/1/Ibrahim%20et%20al%202016.pdf
first_indexed	2018-09-06T12:09:52Z
last_indexed	2018-09-06T12:09:52Z
_version_	1610859982566195200

Multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from a dairy farm

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