The miR-30 microRNA family targets smoothened to regulate hedgehog signalling in zebrafish early muscle development

The importance of microRNAs in development is now widely accepted. However, identifying the specific targets of individual microRNAs and understanding their biological significance remains a major challenge. We have used the zebrafish model system to evaluate the expression and function of microRNAs...

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Main Authors:	Ketley, Ami, Warren, Anne, Holmes, Emily, Gering, Martin, Aboobaker, A. Aziz, Brook, J. David
Format:	Article
Language:	English
Published:	Public Library of Science 2013
Online Access:	http://eprints.nottingham.ac.uk/2902/ http://eprints.nottingham.ac.uk/2902/ http://eprints.nottingham.ac.uk/2902/ http://eprints.nottingham.ac.uk/2902/1/journal.pone.0065170.pdf

id	nottingham-2902
recordtype	eprints
spelling	nottingham-29022018-07-02T09:01:11Z http://eprints.nottingham.ac.uk/2902/ The miR-30 microRNA family targets smoothened to regulate hedgehog signalling in zebrafish early muscle development Ketley, Ami Warren, Anne Holmes, Emily Gering, Martin Aboobaker, A. Aziz Brook, J. David The importance of microRNAs in development is now widely accepted. However, identifying the specific targets of individual microRNAs and understanding their biological significance remains a major challenge. We have used the zebrafish model system to evaluate the expression and function of microRNAs potentially involved in muscle development and study their interaction with predicted target genes. We altered expression of the miR-30 microRNA family and generated phenotypes that mimicked misregulation of the Hedgehog pathway. Inhibition of the miR-30 family increases activity of the pathway, resulting in elevated ptc1 expression and increased numbers of superficial slow-muscle fibres. We show that the transmembrane receptor smoothened is a target of this microRNA family. Our results indicate that fine coordination of smoothened activity by the miR-30 family allows the correct specification and differentiation of distinct muscle cell types during zebrafish embryonic development. Public Library of Science 2013-06 Article PeerReviewed application/pdf en cc_by http://eprints.nottingham.ac.uk/2902/1/journal.pone.0065170.pdf Ketley, Ami and Warren, Anne and Holmes, Emily and Gering, Martin and Aboobaker, A. Aziz and Brook, J. David (2013) The miR-30 microRNA family targets smoothened to regulate hedgehog signalling in zebrafish early muscle development. PLoS ONE, 8 (6). e65170/1-e65170/9. ISSN 1932-6203 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0065170 doi:10.1371/journal.pone.0065170 doi:10.1371/journal.pone.0065170
repository_type	Digital Repository
institution_category	Local University
institution	University of Nottingham Malaysia Campus
building	Nottingham Research Data Repository
collection	Online Access
language	English
description	The importance of microRNAs in development is now widely accepted. However, identifying the specific targets of individual microRNAs and understanding their biological significance remains a major challenge. We have used the zebrafish model system to evaluate the expression and function of microRNAs potentially involved in muscle development and study their interaction with predicted target genes. We altered expression of the miR-30 microRNA family and generated phenotypes that mimicked misregulation of the Hedgehog pathway. Inhibition of the miR-30 family increases activity of the pathway, resulting in elevated ptc1 expression and increased numbers of superficial slow-muscle fibres. We show that the transmembrane receptor smoothened is a target of this microRNA family. Our results indicate that fine coordination of smoothened activity by the miR-30 family allows the correct specification and differentiation of distinct muscle cell types during zebrafish embryonic development.
format	Article
author	Ketley, Ami Warren, Anne Holmes, Emily Gering, Martin Aboobaker, A. Aziz Brook, J. David
spellingShingle	Ketley, Ami Warren, Anne Holmes, Emily Gering, Martin Aboobaker, A. Aziz Brook, J. David The miR-30 microRNA family targets smoothened to regulate hedgehog signalling in zebrafish early muscle development
author_facet	Ketley, Ami Warren, Anne Holmes, Emily Gering, Martin Aboobaker, A. Aziz Brook, J. David
author_sort	Ketley, Ami
title	The miR-30 microRNA family targets smoothened to regulate hedgehog signalling in zebrafish early muscle development
title_short	The miR-30 microRNA family targets smoothened to regulate hedgehog signalling in zebrafish early muscle development
title_full	The miR-30 microRNA family targets smoothened to regulate hedgehog signalling in zebrafish early muscle development
title_fullStr	The miR-30 microRNA family targets smoothened to regulate hedgehog signalling in zebrafish early muscle development
title_full_unstemmed	The miR-30 microRNA family targets smoothened to regulate hedgehog signalling in zebrafish early muscle development
title_sort	mir-30 microrna family targets smoothened to regulate hedgehog signalling in zebrafish early muscle development
publisher	Public Library of Science
publishDate	2013
url	http://eprints.nottingham.ac.uk/2902/ http://eprints.nottingham.ac.uk/2902/ http://eprints.nottingham.ac.uk/2902/ http://eprints.nottingham.ac.uk/2902/1/journal.pone.0065170.pdf
first_indexed	2018-09-06T10:26:48Z
last_indexed	2018-09-06T10:26:48Z
_version_	1610853498226737152

The miR-30 microRNA family targets smoothened to regulate hedgehog signalling in zebrafish early muscle development

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