18S rRNA is a reliable normalisation gene for real time PCR based on influenza virus infected cells

Background: One requisite of quantitative reverse transcription PCR (qRT-PCR) is to normalise the data with an internal reference gene that is invariant regardless of treatment, such as virus infection. Several studies have found variability in the expression of commonly used housekeeping genes, s...

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Main Authors: Kuchipudi, Suresh V., Tellabati, Meenu, Nelli, Rahul K., White, Gavin A., Baquero Perez, Belinda, Sebastian, Sujith, Slomka, Marek J., Brookes, Sharon M., Brown, Ian H., Dunham, Stephen P., Chang, Kin-Chow
Format: Article
Language:English
Published: BioMed Central 2012
Online Access:http://eprints.nottingham.ac.uk/2790/
http://eprints.nottingham.ac.uk/2790/
http://eprints.nottingham.ac.uk/2790/
http://eprints.nottingham.ac.uk/2790/1/Kuchipudi_18S.pdf