Altered placental expression of kisspeptin and its receptor in pre-eclampsia

Kisspeptin, originally identified as metastatin, important in preventing cancer metastasis, has more recently been shown to be important in pregnancy. Roles indicated for kisspeptin in pregnancy include regulating trophoblast invasion and migration during placentation. The pregnancy-specific disorde...

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Main Authors:	Cartwright, Judith E., Williams, Paula Juliet
Format:	Article
Language:	English
Published:	BioScientifica 2012
Online Access:	http://eprints.nottingham.ac.uk/2421/ http://eprints.nottingham.ac.uk/2421/ http://eprints.nottingham.ac.uk/2421/ http://eprints.nottingham.ac.uk/2421/1/williams_altered.pdf

id	nottingham-2421
recordtype	eprints
spelling	nottingham-24212017-10-15T13:31:51Z http://eprints.nottingham.ac.uk/2421/ Altered placental expression of kisspeptin and its receptor in pre-eclampsia Cartwright, Judith E. Williams, Paula Juliet Kisspeptin, originally identified as metastatin, important in preventing cancer metastasis, has more recently been shown to be important in pregnancy. Roles indicated for kisspeptin in pregnancy include regulating trophoblast invasion and migration during placentation. The pregnancy-specific disorder pre-eclampsia (PE) is now accepted to begin with inadequate trophoblast invasion and the current study therefore sets out to characterise placental expression of both kisspeptin (KISS1) and its receptor (KISS1R) throughout pregnancy and in PE. Placental tissue was obtained from women undergoing elective surgical termination of early pregnancy (n=10) and from women following Caesarean section at term in normal pregnancy (n=10) and with PE (n=10). Immunohistochemistry of paraffin embedded sections and western immunoblotting were performed to assess protein localisation and expression. Quantitative real-time PCR was carried out to evaluate mRNA expression of both KISS1 and KISS1R. Protein and mRNA expression was found to mirror each other with KISS1 expression found to be reduced in PE compared with that in normal term pregnancy. Interestingly, KISS1R expression at both the mRNA and protein levels was found to be increased in PE compared with that in normal term pregnancy. The current findings of increased KISS1R expression may represent a mechanism by which functional activity of KISS1 is higher in PE than in normal pregnancy. Higher levels of activity of KISS1R may be involved in inhibition of trophoblast invasion and angiogenesis, which are associated with PE. BioScientifica 2012-07 Article PeerReviewed application/pdf en cc_by http://eprints.nottingham.ac.uk/2421/1/williams_altered.pdf Cartwright, Judith E. and Williams, Paula Juliet (2012) Altered placental expression of kisspeptin and its receptor in pre-eclampsia. Journal of Endocrinology, 214 (1). pp. 79-85. ISSN 0022-0795 http://joe.endocrinology-journals.org/content/214/1/79 doi:10.1530/JOE-12-0091 doi:10.1530/JOE-12-0091
repository_type	Digital Repository
institution_category	Local University
institution	University of Nottingham Malaysia Campus
building	Nottingham Research Data Repository
collection	Online Access
language	English
description	Kisspeptin, originally identified as metastatin, important in preventing cancer metastasis, has more recently been shown to be important in pregnancy. Roles indicated for kisspeptin in pregnancy include regulating trophoblast invasion and migration during placentation. The pregnancy-specific disorder pre-eclampsia (PE) is now accepted to begin with inadequate trophoblast invasion and the current study therefore sets out to characterise placental expression of both kisspeptin (KISS1) and its receptor (KISS1R) throughout pregnancy and in PE. Placental tissue was obtained from women undergoing elective surgical termination of early pregnancy (n=10) and from women following Caesarean section at term in normal pregnancy (n=10) and with PE (n=10). Immunohistochemistry of paraffin embedded sections and western immunoblotting were performed to assess protein localisation and expression. Quantitative real-time PCR was carried out to evaluate mRNA expression of both KISS1 and KISS1R. Protein and mRNA expression was found to mirror each other with KISS1 expression found to be reduced in PE compared with that in normal term pregnancy. Interestingly, KISS1R expression at both the mRNA and protein levels was found to be increased in PE compared with that in normal term pregnancy. The current findings of increased KISS1R expression may represent a mechanism by which functional activity of KISS1 is higher in PE than in normal pregnancy. Higher levels of activity of KISS1R may be involved in inhibition of trophoblast invasion and angiogenesis, which are associated with PE.
format	Article
author	Cartwright, Judith E. Williams, Paula Juliet
spellingShingle	Cartwright, Judith E. Williams, Paula Juliet Altered placental expression of kisspeptin and its receptor in pre-eclampsia
author_facet	Cartwright, Judith E. Williams, Paula Juliet
author_sort	Cartwright, Judith E.
title	Altered placental expression of kisspeptin and its receptor in pre-eclampsia
title_short	Altered placental expression of kisspeptin and its receptor in pre-eclampsia
title_full	Altered placental expression of kisspeptin and its receptor in pre-eclampsia
title_fullStr	Altered placental expression of kisspeptin and its receptor in pre-eclampsia
title_full_unstemmed	Altered placental expression of kisspeptin and its receptor in pre-eclampsia
title_sort	altered placental expression of kisspeptin and its receptor in pre-eclampsia
publisher	BioScientifica
publishDate	2012
url	http://eprints.nottingham.ac.uk/2421/ http://eprints.nottingham.ac.uk/2421/ http://eprints.nottingham.ac.uk/2421/ http://eprints.nottingham.ac.uk/2421/1/williams_altered.pdf
first_indexed	2018-09-06T10:22:59Z
last_indexed	2018-09-06T10:22:59Z
_version_	1610853257793503232

Altered placental expression of kisspeptin and its receptor in pre-eclampsia

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