Antibiotic susceptibility study Of metal-gentamicin complexes against Staphylococcus aureus biofilms

Introduction: Dental plaque is a structurally and functionally organized biofilm. Modern molecular biological techniques have identified about 1000 different bacterial species in the dental biofilm, twice as many as can be cultured. Inherent resistance of biofilm bacteria to conventional antibiot...

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Main Authors:	Shahdan, Intan Azura, Mohd Sobri, Fatimah Zahrah, Hanapi, Mohammad Faiz Hizzuan, Ahmad Yusof @ Hanafi, Hanani, How, Fiona Ni Foong
Format:	Article
Language:	English
Published:	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2017
Subjects:	QD Chemistry
Online Access:	http://irep.iium.edu.my/65146/ http://irep.iium.edu.my/65146/ http://irep.iium.edu.my/65146/1/The%20International%20Medical%20Journal%20Malaysia%20%282017%29%20Vol16%20Supplementary%20issue%20no2.pdf

id	iium-65146
recordtype	eprints
spelling	iium-651462018-08-16T08:19:58Z http://irep.iium.edu.my/65146/ Antibiotic susceptibility study Of metal-gentamicin complexes against Staphylococcus aureus biofilms Shahdan, Intan Azura Mohd Sobri, Fatimah Zahrah Hanapi, Mohammad Faiz Hizzuan Ahmad Yusof @ Hanafi, Hanani How, Fiona Ni Foong QD Chemistry Introduction: Dental plaque is a structurally and functionally organized biofilm. Modern molecular biological techniques have identified about 1000 different bacterial species in the dental biofilm, twice as many as can be cultured. Inherent resistance of biofilm bacteria to conventional antibiotics is alarming. It induces antibiotic resistance to an order of three or more in magnitude greater than those displayed by planktonic bacteria. Staphylococcus aureus is the most dominant bacterial species isolated from the saliva and dental plaques. One of the reasons for its pathogenicity is its ability to form biofilms. In this study, the resistance of S. aureus biofilms against a eries of metal-antibiotics, an alternative to the conventional antibiotics, was investigated. Materials and Methods: A series of metal-antibiotic complexes derived from gentamicin was synthesized to give metal-gentamicin complexes. The metal contents of all the compounds were determined using Atomic Absorption Spectroscopy (AAS). Antibiotic susceptibility testing of the gentamicin-antibiotic complexes against several strains of S. aureus biofilms was conducted using broth microdilution assay. Results: The results showed that S. aureus is susceptible against Co(II) and Fe(II) gentamicin complexes; all were tested at 0.25 to 1 mmol concentrations. Conclusion(s): Co(II) and Fe(II)-gentamicin complexes demonstrated antimicrobial activity. Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2017-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/65146/1/The%20International%20Medical%20Journal%20Malaysia%20%282017%29%20Vol16%20Supplementary%20issue%20no2.pdf Shahdan, Intan Azura and Mohd Sobri, Fatimah Zahrah and Hanapi, Mohammad Faiz Hizzuan and Ahmad Yusof @ Hanafi, Hanani and How, Fiona Ni Foong (2017) Antibiotic susceptibility study Of metal-gentamicin complexes against Staphylococcus aureus biofilms. International Medical Journal Malaysia, 16 (Supplementary Issue 2). p. 1. http://iiumedic.net/imjm/v1/download/Volume%2016%20No%201%20Supp%202/ID06_IMJM-Vol16-Supp2.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Shahdan, Intan Azura Mohd Sobri, Fatimah Zahrah Hanapi, Mohammad Faiz Hizzuan Ahmad Yusof @ Hanafi, Hanani How, Fiona Ni Foong Antibiotic susceptibility study Of metal-gentamicin complexes against Staphylococcus aureus biofilms
description	Introduction: Dental plaque is a structurally and functionally organized biofilm. Modern molecular biological techniques have identified about 1000 different bacterial species in the dental biofilm, twice as many as can be cultured. Inherent resistance of biofilm bacteria to conventional antibiotics is alarming. It induces antibiotic resistance to an order of three or more in magnitude greater than those displayed by planktonic bacteria. Staphylococcus aureus is the most dominant bacterial species isolated from the saliva and dental plaques. One of the reasons for its pathogenicity is its ability to form biofilms. In this study, the resistance of S. aureus biofilms against a eries of metal-antibiotics, an alternative to the conventional antibiotics, was investigated. Materials and Methods: A series of metal-antibiotic complexes derived from gentamicin was synthesized to give metal-gentamicin complexes. The metal contents of all the compounds were determined using Atomic Absorption Spectroscopy (AAS). Antibiotic susceptibility testing of the gentamicin-antibiotic complexes against several strains of S. aureus biofilms was conducted using broth microdilution assay. Results: The results showed that S. aureus is susceptible against Co(II) and Fe(II) gentamicin complexes; all were tested at 0.25 to 1 mmol concentrations. Conclusion(s): Co(II) and Fe(II)-gentamicin complexes demonstrated antimicrobial activity.
format	Article
author	Shahdan, Intan Azura Mohd Sobri, Fatimah Zahrah Hanapi, Mohammad Faiz Hizzuan Ahmad Yusof @ Hanafi, Hanani How, Fiona Ni Foong
author_facet	Shahdan, Intan Azura Mohd Sobri, Fatimah Zahrah Hanapi, Mohammad Faiz Hizzuan Ahmad Yusof @ Hanafi, Hanani How, Fiona Ni Foong
author_sort	Shahdan, Intan Azura
title	Antibiotic susceptibility study Of metal-gentamicin complexes against Staphylococcus aureus biofilms
title_short	Antibiotic susceptibility study Of metal-gentamicin complexes against Staphylococcus aureus biofilms
title_full	Antibiotic susceptibility study Of metal-gentamicin complexes against Staphylococcus aureus biofilms
title_fullStr	Antibiotic susceptibility study Of metal-gentamicin complexes against Staphylococcus aureus biofilms
title_full_unstemmed	Antibiotic susceptibility study Of metal-gentamicin complexes against Staphylococcus aureus biofilms
title_sort	antibiotic susceptibility study of metal-gentamicin complexes against staphylococcus aureus biofilms
publisher	Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia
publishDate	2017
url	http://irep.iium.edu.my/65146/ http://irep.iium.edu.my/65146/ http://irep.iium.edu.my/65146/1/The%20International%20Medical%20Journal%20Malaysia%20%282017%29%20Vol16%20Supplementary%20issue%20no2.pdf
first_indexed	2018-09-07T08:22:12Z
last_indexed	2018-09-07T08:22:12Z
_version_	1610936255600656384

Antibiotic susceptibility study Of metal-gentamicin complexes against Staphylococcus aureus biofilms

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