Isolation and characterization of biosurfactant-producing bacteria isolated from petroleum contaminated sites with the potential to be used in bioremediation

Surfactants have been widely used to facilitate biodegradation of petroleum pollutant; particularly those are being produced by microorganisms which are also known as biosurfactant. Six bacterial isolates from petroleum contaminated sites were found to be potential hydrocarbon degrader candidates...

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Main Authors:	Mohamad Zainal, Noor Shaidatul Lyana, Mohd Omar, Suhaila, Mohd Ashaaari, Mardiana
Format:	Article
Language:	English
Published:	Razi Publishing 2017
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/62360/ http://irep.iium.edu.my/62360/ http://irep.iium.edu.my/62360/1/Mardiana_2017.pdf

id	iium-62360
recordtype	eprints
spelling	iium-623602018-03-02T03:05:32Z http://irep.iium.edu.my/62360/ Isolation and characterization of biosurfactant-producing bacteria isolated from petroleum contaminated sites with the potential to be used in bioremediation Mohamad Zainal, Noor Shaidatul Lyana Mohd Omar, Suhaila Mohd Ashaaari, Mardiana QR Microbiology Surfactants have been widely used to facilitate biodegradation of petroleum pollutant; particularly those are being produced by microorganisms which are also known as biosurfactant. Six bacterial isolates from petroleum contaminated sites were found to be potential hydrocarbon degrader candidates based on their ability to grow on minimal media supplemented with petroleum crude oil as sole carbon source. In addition, they also found to be having potential in producing extracellular biosurfactant based on the results of screening for biosurfactant activity. These isolates were further identified and characterized. Morphological and biochemical characteristics of these isolates have been examined and molecular identification was done by amplifying 16S rRNA gene. Three isolates were identified as the member of the genus Pseudomonas; another two isolates were the member of genus Comamonas and one isolate from the genus Stenotrophomonas. Several qualitative screening methods (microplate assay, oil displacement test, emulsification assay and drop-collapse test) showed variation of results; suggesting the need to support these findings with quantitative screening based on measurement of surface activity. BSP 6 which was found to be closely related to Comamonas aquatica was the most promising biosurfactant producer found in this study based on two positive results out of four qualitative screening methods performed. Razi Publishing 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/62360/1/Mardiana_2017.pdf Mohamad Zainal, Noor Shaidatul Lyana and Mohd Omar, Suhaila and Mohd Ashaaari, Mardiana (2017) Isolation and characterization of biosurfactant-producing bacteria isolated from petroleum contaminated sites with the potential to be used in bioremediation. Science Heritage Journal / Galeri Warisan Sains (GWS), 1 (2). pp. 11-15. ISSN 2521-0858 https://zibelinepub.com/archives/2gws2017/2gws2017-11-15.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Mohamad Zainal, Noor Shaidatul Lyana Mohd Omar, Suhaila Mohd Ashaaari, Mardiana Isolation and characterization of biosurfactant-producing bacteria isolated from petroleum contaminated sites with the potential to be used in bioremediation
description	Surfactants have been widely used to facilitate biodegradation of petroleum pollutant; particularly those are being produced by microorganisms which are also known as biosurfactant. Six bacterial isolates from petroleum contaminated sites were found to be potential hydrocarbon degrader candidates based on their ability to grow on minimal media supplemented with petroleum crude oil as sole carbon source. In addition, they also found to be having potential in producing extracellular biosurfactant based on the results of screening for biosurfactant activity. These isolates were further identified and characterized. Morphological and biochemical characteristics of these isolates have been examined and molecular identification was done by amplifying 16S rRNA gene. Three isolates were identified as the member of the genus Pseudomonas; another two isolates were the member of genus Comamonas and one isolate from the genus Stenotrophomonas. Several qualitative screening methods (microplate assay, oil displacement test, emulsification assay and drop-collapse test) showed variation of results; suggesting the need to support these findings with quantitative screening based on measurement of surface activity. BSP 6 which was found to be closely related to Comamonas aquatica was the most promising biosurfactant producer found in this study based on two positive results out of four qualitative screening methods performed.
format	Article
author	Mohamad Zainal, Noor Shaidatul Lyana Mohd Omar, Suhaila Mohd Ashaaari, Mardiana
author_facet	Mohamad Zainal, Noor Shaidatul Lyana Mohd Omar, Suhaila Mohd Ashaaari, Mardiana
author_sort	Mohamad Zainal, Noor Shaidatul Lyana
title	Isolation and characterization of biosurfactant-producing bacteria isolated from petroleum contaminated sites with the potential to be used in bioremediation
title_short	Isolation and characterization of biosurfactant-producing bacteria isolated from petroleum contaminated sites with the potential to be used in bioremediation
title_full	Isolation and characterization of biosurfactant-producing bacteria isolated from petroleum contaminated sites with the potential to be used in bioremediation
title_fullStr	Isolation and characterization of biosurfactant-producing bacteria isolated from petroleum contaminated sites with the potential to be used in bioremediation
title_full_unstemmed	Isolation and characterization of biosurfactant-producing bacteria isolated from petroleum contaminated sites with the potential to be used in bioremediation
title_sort	isolation and characterization of biosurfactant-producing bacteria isolated from petroleum contaminated sites with the potential to be used in bioremediation
publisher	Razi Publishing
publishDate	2017
url	http://irep.iium.edu.my/62360/ http://irep.iium.edu.my/62360/ http://irep.iium.edu.my/62360/1/Mardiana_2017.pdf
first_indexed	2018-09-07T08:07:47Z
last_indexed	2018-09-07T08:07:47Z
_version_	1610935349245116416

Isolation and characterization of biosurfactant-producing bacteria isolated from petroleum contaminated sites with the potential to be used in bioremediation

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