Quantitative evaluation of p16INK4a promoter methylation using pyrosequencing in de novo diffuse large B-cell lymphoma

The p16INK4a tumor suppressor gene can be inactivated by a variety of events including promoter hypermethylation. In diffuse large B-cell lymphoma (DLBCL), p16INK4a methylation has been associated with advanced disease stage and higher IPI. The prognostic impact of p16INK4a methylation in DLBCL rem...

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Main Authors:	Zainuddin, Norafiza, Kanduri, Meena, Berglund, Mattias, Lindell, Monica, Amini, Rose-Marie, Roos, Göran, Sundström, Christer, Enblad , Gunilla, Rosenquist, Richard
Format:	Article
Language:	English
Published:	Pergamon 2011
Subjects:	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Online Access:	http://irep.iium.edu.my/4588/ http://irep.iium.edu.my/4588/ http://irep.iium.edu.my/4588/ http://irep.iium.edu.my/4588/1/p16.pdf

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recordtype	eprints
spelling	iium-45882013-06-20T03:00:04Z http://irep.iium.edu.my/4588/ Quantitative evaluation of p16INK4a promoter methylation using pyrosequencing in de novo diffuse large B-cell lymphoma Zainuddin, Norafiza Kanduri, Meena Berglund, Mattias Lindell, Monica Amini, Rose-Marie Roos, Göran Sundström, Christer Enblad , Gunilla Rosenquist, Richard RC0254 Neoplasms. Tumors. Oncology (including Cancer) The p16INK4a tumor suppressor gene can be inactivated by a variety of events including promoter hypermethylation. In diffuse large B-cell lymphoma (DLBCL), p16INK4a methylation has been associated with advanced disease stage and higher IPI. The prognostic impact of p16INK4a methylation in DLBCL remains unclear; however, it has been suggested to correlate with inferior outcome. To further investigate the clinical impact of p16INK4a methylation in DLBCL, promoter methylation of this gene was assessed quantitatively by pyrosequencing. Forty-two of 113 (37%) DLBCL patients with methylation level above 5% were categorized as methylated and subsequently divided into low, intermediate and high methylation categories. Overall, no association was shown between the extent of p16INK4a methylation and patients’ clinical characteristics, except disease stage (P = 0.049). Moreover, we could not reveal any impact of p16INK4a methylation on lymphoma-specific survival. Although >25% of p16INK4a methylation correlated with a better progression-free survival (P = 0.048) in patients <65 years old, the significance of this finding, if any, needs to be further investigated. In conclusion, our finding questions the role of p16INK4a promoter methylation as a negative prognostic factor in DLBCL. Pergamon 2011-04-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/4588/1/p16.pdf Zainuddin, Norafiza and Kanduri, Meena and Berglund, Mattias and Lindell, Monica and Amini, Rose-Marie and Roos, Göran and Sundström, Christer and Enblad , Gunilla and Rosenquist, Richard (2011) Quantitative evaluation of p16INK4a promoter methylation using pyrosequencing in de novo diffuse large B-cell lymphoma. Leukemia Research, 35 (4). pp. 438-443. ISSN 0145-2126 http://www.sciencedirect.com/science?_ob=ArticleListURL&_method=list&_ArticleListID=1815164858&_sort=r&_st=13&view=c&_acct=C000028758&_version=1&_urlVersion=0&_userid=565570&md5=5d40e637a60dab2a625f6a7b0bd78796&searchtype=a 10.1016/j.leukres.2010.10.001
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
spellingShingle	RC0254 Neoplasms. Tumors. Oncology (including Cancer) Zainuddin, Norafiza Kanduri, Meena Berglund, Mattias Lindell, Monica Amini, Rose-Marie Roos, Göran Sundström, Christer Enblad , Gunilla Rosenquist, Richard Quantitative evaluation of p16INK4a promoter methylation using pyrosequencing in de novo diffuse large B-cell lymphoma
description	The p16INK4a tumor suppressor gene can be inactivated by a variety of events including promoter hypermethylation. In diffuse large B-cell lymphoma (DLBCL), p16INK4a methylation has been associated with advanced disease stage and higher IPI. The prognostic impact of p16INK4a methylation in DLBCL remains unclear; however, it has been suggested to correlate with inferior outcome. To further investigate the clinical impact of p16INK4a methylation in DLBCL, promoter methylation of this gene was assessed quantitatively by pyrosequencing. Forty-two of 113 (37%) DLBCL patients with methylation level above 5% were categorized as methylated and subsequently divided into low, intermediate and high methylation categories. Overall, no association was shown between the extent of p16INK4a methylation and patients’ clinical characteristics, except disease stage (P = 0.049). Moreover, we could not reveal any impact of p16INK4a methylation on lymphoma-specific survival. Although >25% of p16INK4a methylation correlated with a better progression-free survival (P = 0.048) in patients <65 years old, the significance of this finding, if any, needs to be further investigated. In conclusion, our finding questions the role of p16INK4a promoter methylation as a negative prognostic factor in DLBCL.
format	Article
author	Zainuddin, Norafiza Kanduri, Meena Berglund, Mattias Lindell, Monica Amini, Rose-Marie Roos, Göran Sundström, Christer Enblad , Gunilla Rosenquist, Richard
author_facet	Zainuddin, Norafiza Kanduri, Meena Berglund, Mattias Lindell, Monica Amini, Rose-Marie Roos, Göran Sundström, Christer Enblad , Gunilla Rosenquist, Richard
author_sort	Zainuddin, Norafiza
title	Quantitative evaluation of p16INK4a promoter methylation using pyrosequencing in de novo diffuse large B-cell lymphoma
title_short	Quantitative evaluation of p16INK4a promoter methylation using pyrosequencing in de novo diffuse large B-cell lymphoma
title_full	Quantitative evaluation of p16INK4a promoter methylation using pyrosequencing in de novo diffuse large B-cell lymphoma
title_fullStr	Quantitative evaluation of p16INK4a promoter methylation using pyrosequencing in de novo diffuse large B-cell lymphoma
title_full_unstemmed	Quantitative evaluation of p16INK4a promoter methylation using pyrosequencing in de novo diffuse large B-cell lymphoma
title_sort	quantitative evaluation of p16ink4a promoter methylation using pyrosequencing in de novo diffuse large b-cell lymphoma
publisher	Pergamon
publishDate	2011
url	http://irep.iium.edu.my/4588/ http://irep.iium.edu.my/4588/ http://irep.iium.edu.my/4588/ http://irep.iium.edu.my/4588/1/p16.pdf
first_indexed	2018-09-07T03:11:55Z
last_indexed	2018-09-07T03:11:55Z
_version_	1610916734716346368

Quantitative evaluation of p16INK4a promoter methylation using pyrosequencing in de novo diffuse large B-cell lymphoma

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