Development and validation of reversed phase high performance liquid chromatographic method for determination of amlodipine
Purpose: To develop and validate a new sensitive and low-cost method for the analysis of amlodipine in tablet dosage form using reversed phase high performance liquid chromatography (RP-HPLC) with ultraviolet (UV) detection. Methods: Standards and samples were prepared by dissolving amlodipine be...
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| Format: | Article |
| Language: | English |
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Pharmacotherapy Group, Faculty of Pharmacy, University of Benin
2015
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| Online Access: | http://irep.iium.edu.my/43812/ http://irep.iium.edu.my/43812/ http://irep.iium.edu.my/43812/1/TJPR_Amlodipine_2015.pdf |
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iium-438122017-11-08T09:33:59Z http://irep.iium.edu.my/43812/ Development and validation of reversed phase high performance liquid chromatographic method for determination of amlodipine Alaama, Mohamed Helaluddin , Abul Bashar Mohammed Mohamad , Huda Jamilah Amiruddin , Noor Syafawati SA, Abbas QD Chemistry RS Pharmacy and materia medica Purpose: To develop and validate a new sensitive and low-cost method for the analysis of amlodipine in tablet dosage form using reversed phase high performance liquid chromatography (RP-HPLC) with ultraviolet (UV) detection. Methods: Standards and samples were prepared by dissolving amlodipine besylate standard or amlodipine tablets in mobile phase and sonicated for 5 min. The samples were analysed by RP-HPLC equipped with quaternary pump and auto-injector. Separation was achieved using C18 column, and the mobile phase consisted of ammonium acetate buffer containing 0.02 % triethylamine TEA (pH = 4, adjusted using glacial acetic acid) and acetonitrile in the ratio 60:40 v/v. The flow rate was 1 ml/min and a UV detector was used for the detection of amlodipine at a wavelength of 248 nm. The method was validated according to International Conference of Harmonization (ICH) guidelines. Results: The retention time for amlodipine peak was 3.44 ± 0.41 min with a total run time of 6 min. The method was linear over the range of 0.5 - 40 μg/ml with coefficient of determination (R2) of 0.999. Recovery was 98.09 - 100.19 %, and the method showed high precision and repeatability. All validated parameters were within the range of ICH requirements. Conclusion: A new rapid sensitive and low-cost method has been developed and validated for the analysis of amlodipine in tablet dosage form. Pharmacotherapy Group, Faculty of Pharmacy, University of Benin 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/43812/1/TJPR_Amlodipine_2015.pdf Alaama, Mohamed and Helaluddin , Abul Bashar Mohammed and Mohamad , Huda Jamilah and Amiruddin , Noor Syafawati and SA, Abbas (2015) Development and validation of reversed phase high performance liquid chromatographic method for determination of amlodipine. Tropical Journal of Pharmaceutical Research, 14 (4). pp. 663-669. ISSN 1596-9827 (O), 1596-5996 (P) http://dx.doi.org/10.4314/tjpr.v14i4.15 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English |
| topic |
QD Chemistry RS Pharmacy and materia medica |
| spellingShingle |
QD Chemistry RS Pharmacy and materia medica Alaama, Mohamed Helaluddin , Abul Bashar Mohammed Mohamad , Huda Jamilah Amiruddin , Noor Syafawati SA, Abbas Development and validation of reversed phase high performance liquid chromatographic method for determination of amlodipine |
| description |
Purpose: To develop and validate a new sensitive and low-cost method for the analysis of amlodipine
in tablet dosage form using reversed phase high performance liquid chromatography (RP-HPLC) with
ultraviolet (UV) detection.
Methods: Standards and samples were prepared by dissolving amlodipine besylate standard or
amlodipine tablets in mobile phase and sonicated for 5 min. The samples were analysed by RP-HPLC
equipped with quaternary pump and auto-injector. Separation was achieved using C18 column, and the
mobile phase consisted of ammonium acetate buffer containing 0.02 % triethylamine TEA (pH = 4,
adjusted using glacial acetic acid) and acetonitrile in the ratio 60:40 v/v. The flow rate was 1 ml/min and
a UV detector was used for the detection of amlodipine at a wavelength of 248 nm. The method was
validated according to International Conference of Harmonization (ICH) guidelines.
Results: The retention time for amlodipine peak was 3.44 ± 0.41 min with a total run time of 6 min. The
method was linear over the range of 0.5 - 40 μg/ml with coefficient of determination (R2) of 0.999.
Recovery was 98.09 - 100.19 %, and the method showed high precision and repeatability. All validated
parameters were within the range of ICH requirements.
Conclusion: A new rapid sensitive and low-cost method has been developed and validated for the
analysis of amlodipine in tablet dosage form. |
| format |
Article |
| author |
Alaama, Mohamed Helaluddin , Abul Bashar Mohammed Mohamad , Huda Jamilah Amiruddin , Noor Syafawati SA, Abbas |
| author_facet |
Alaama, Mohamed Helaluddin , Abul Bashar Mohammed Mohamad , Huda Jamilah Amiruddin , Noor Syafawati SA, Abbas |
| author_sort |
Alaama, Mohamed |
| title |
Development and validation of reversed phase high
performance liquid chromatographic method for
determination of amlodipine |
| title_short |
Development and validation of reversed phase high
performance liquid chromatographic method for
determination of amlodipine |
| title_full |
Development and validation of reversed phase high
performance liquid chromatographic method for
determination of amlodipine |
| title_fullStr |
Development and validation of reversed phase high
performance liquid chromatographic method for
determination of amlodipine |
| title_full_unstemmed |
Development and validation of reversed phase high
performance liquid chromatographic method for
determination of amlodipine |
| title_sort |
development and validation of reversed phase high
performance liquid chromatographic method for
determination of amlodipine |
| publisher |
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin |
| publishDate |
2015 |
| url |
http://irep.iium.edu.my/43812/ http://irep.iium.edu.my/43812/ http://irep.iium.edu.my/43812/1/TJPR_Amlodipine_2015.pdf |
| first_indexed |
2018-09-07T06:26:15Z |
| last_indexed |
2018-09-07T06:26:15Z |
| _version_ |
1610928960697270272 |