Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains

IBDV outbreak had been reported to infect broiler chicken in Malaysia and cause high mortality. It had been reported that the virulence and pathogenicity of the virus is related to its coated protein, specifically the VP2 hypervariable regions. To study this, the nucleotides and protein of the loca...

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Main Authors:	Ghazali, Mohd. Shahrin, Ahmad Raus, Raha, Mat Amin, Noriha, Shohaimi, Syamsiah Aini, Abu Bakar, Farah Diba, Zainuddin, Norafiza, Nor Muhammad, Nor Azlin Alia
Format:	Article
Language:	English
Published:	Scholars Research Library 2013
Subjects:	QH426 Genetics
Online Access:	http://irep.iium.edu.my/38464/ http://irep.iium.edu.my/38464/ http://irep.iium.edu.my/38464/1/ABR-2013-4-5-92-104.pdf

id	iium-38464
recordtype	eprints
spelling	iium-384642014-09-29T01:29:52Z http://irep.iium.edu.my/38464/ Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains Ghazali, Mohd. Shahrin Ahmad Raus, Raha Mat Amin, Noriha Shohaimi, Syamsiah Aini Abu Bakar, Farah Diba Zainuddin, Norafiza Nor Muhammad, Nor Azlin Alia QH426 Genetics IBDV outbreak had been reported to infect broiler chicken in Malaysia and cause high mortality. It had been reported that the virulence and pathogenicity of the virus is related to its coated protein, specifically the VP2 hypervariable regions. To study this, the nucleotides and protein of the local IBDV isolate was sequenced and analyzed by comparing it with other IBDV that had been reported previously. To obtain the virus sequence, local IBDV isolate strain 3529/92 was propagated in SPF eggs and RNA were extracted, amplify by rt (reverse transcriptase)-PCR before being inserted into pCR 2.1 TOPO for cloning and sequencing. The full length of the gene segment for the coat protein was constructed by concomitantly joining the fragments using the native restrictions sites of IBDV in pTrcHis2a expression vector. The sequence analysis revealed that the local IBDV strain 3529/92 consists of 3039 nucleotides which encodes for 1012 amino acids. BLAST analysis of the nucleotides sequence showed that the local strain shared greatest similarity with the Dutch D6948 vv IBDV subtypes. Analysis on the VP2 variable regions of 3529/92 showed most amino acid substitutions at VP2 variables regions are similar to the changes in VP2 variable regions of vvIBDV subtypes. Phylogenetic analysis prove that that 3529/92 isolates closely related with vvIBDV subtypes. The nucleotides sequences of ORF A2 3529/92 will give great insight in vaccine development against IBDV especially vvIBDV subtypes. Scholars Research Library 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38464/1/ABR-2013-4-5-92-104.pdf Ghazali, Mohd. Shahrin and Ahmad Raus, Raha and Mat Amin, Noriha and Shohaimi, Syamsiah Aini and Abu Bakar, Farah Diba and Zainuddin, Norafiza and Nor Muhammad, Nor Azlin Alia (2013) Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains. Annals of Biological Research, 4 (5). pp. 92-104. ISSN 0976-1233 http://scholarsresearchlibrary.com/ABR-vol4-iss5/ABR-2013-4-5-92-104.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QH426 Genetics
spellingShingle	QH426 Genetics Ghazali, Mohd. Shahrin Ahmad Raus, Raha Mat Amin, Noriha Shohaimi, Syamsiah Aini Abu Bakar, Farah Diba Zainuddin, Norafiza Nor Muhammad, Nor Azlin Alia Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains
description	IBDV outbreak had been reported to infect broiler chicken in Malaysia and cause high mortality. It had been reported that the virulence and pathogenicity of the virus is related to its coated protein, specifically the VP2 hypervariable regions. To study this, the nucleotides and protein of the local IBDV isolate was sequenced and analyzed by comparing it with other IBDV that had been reported previously. To obtain the virus sequence, local IBDV isolate strain 3529/92 was propagated in SPF eggs and RNA were extracted, amplify by rt (reverse transcriptase)-PCR before being inserted into pCR 2.1 TOPO for cloning and sequencing. The full length of the gene segment for the coat protein was constructed by concomitantly joining the fragments using the native restrictions sites of IBDV in pTrcHis2a expression vector. The sequence analysis revealed that the local IBDV strain 3529/92 consists of 3039 nucleotides which encodes for 1012 amino acids. BLAST analysis of the nucleotides sequence showed that the local strain shared greatest similarity with the Dutch D6948 vv IBDV subtypes. Analysis on the VP2 variable regions of 3529/92 showed most amino acid substitutions at VP2 variables regions are similar to the changes in VP2 variable regions of vvIBDV subtypes. Phylogenetic analysis prove that that 3529/92 isolates closely related with vvIBDV subtypes. The nucleotides sequences of ORF A2 3529/92 will give great insight in vaccine development against IBDV especially vvIBDV subtypes.
format	Article
author	Ghazali, Mohd. Shahrin Ahmad Raus, Raha Mat Amin, Noriha Shohaimi, Syamsiah Aini Abu Bakar, Farah Diba Zainuddin, Norafiza Nor Muhammad, Nor Azlin Alia
author_facet	Ghazali, Mohd. Shahrin Ahmad Raus, Raha Mat Amin, Noriha Shohaimi, Syamsiah Aini Abu Bakar, Farah Diba Zainuddin, Norafiza Nor Muhammad, Nor Azlin Alia
author_sort	Ghazali, Mohd. Shahrin
title	Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains
title_short	Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains
title_full	Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains
title_fullStr	Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains
title_full_unstemmed	Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains
title_sort	genomic analysis and comparison of very virulent infectious bursal disease virus (vvibdv) affecting malaysian poultry chickens with other ibdv strains
publisher	Scholars Research Library
publishDate	2013
url	http://irep.iium.edu.my/38464/ http://irep.iium.edu.my/38464/ http://irep.iium.edu.my/38464/1/ABR-2013-4-5-92-104.pdf
first_indexed	2018-09-07T05:58:49Z
last_indexed	2018-09-07T05:58:49Z
_version_	1610927234597519360

Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains

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