Hippocampal GFAP and MAP2 mRNA expressions of curry leaves extract treatment in 2VO rats of neurodegeneration

Background: Experimental neurodegeneration can be induced by two-vessel occlusion intervention (2VO). Nigella sativa (Linn.) seeds extract (NSSE) has recently shown to enhance memory and learning task performances in rats with 2VO. Prevention of neuroinflammation and/or of toxic free radical formati...

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Main Authors:	Babar, Z.M., Azzubaidi, Marwan Saad, Sulaiman , Wan Azizi, Talib, Norlelawati A.
Format:	Conference or Workshop Item
Language:	English
Published:	2014
Subjects:	RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action
Online Access:	http://irep.iium.edu.my/36150/ http://irep.iium.edu.my/36150/ http://irep.iium.edu.my/36150/1/COnference_2014.pdf

id	iium-36150
recordtype	eprints
spelling	iium-361502014-04-16T06:27:14Z http://irep.iium.edu.my/36150/ Hippocampal GFAP and MAP2 mRNA expressions of curry leaves extract treatment in 2VO rats of neurodegeneration Babar, Z.M. Azzubaidi, Marwan Saad Sulaiman , Wan Azizi Talib, Norlelawati A. RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action Background: Experimental neurodegeneration can be induced by two-vessel occlusion intervention (2VO). Nigella sativa (Linn.) seeds extract (NSSE) has recently shown to enhance memory and learning task performances in rats with 2VO. Prevention of neuroinflammation and/or of toxic free radical formation are possible mechanisms. The glial fibrillary acidic protein (GFAP) is a vital neuroinflammatory marker in neuroscience researches. GFAP was found to be up-regulated in 2VO rats indicating astrogliosis. Microtubule associated protein type II (MAP2) is an important marker for neuroprotection during and after ischemic brain injury or hypoxia. It was found to be down-regulated in 2VO rats. Objective: The current study aimed to compare the hippocampal MAP-2 and GFAP mRNA expressions of 2VO (untreated) male rats in the experimental neurodegeneration with other groups of rats treated with NSSE. Method: 18 adult male Sprague Dawley rats were randomly divided into 3 groups (n=6); Healthy Control (HC); 2VO- without treatment (2VO); 2VO+NSSE-treated (NSSE). The NSSE group was pre-treated (2ml, 50 mg/kg/day, orally) for 10 days prior to 2VO surgery and continued until all animals were sacrificed at the end of 10th postoperative week. Hippocampal samples were then collected; total RNA was extracted, purified and relatively quantified via reverse transcribed cDNA as per ∆∆Cq of RT-qPCR assay. Results: There was a significant 3 folds difference of GFAP mRNA expression in both HC and NSSE groups as compared to that of 2VO. However, GFAP mRNA expression difference was not significant (P=0.52) of for NSSE vs. HC. Hippocampal MAP2 mRNA expression of NSSE group appeared to be insignificantly different from the untreated 2VO group. Conclusion: Prolonged treatment with NSSE has the potential to prevent hypoperfusion-induced neuroinflammation within the hippocampus. This may be a potential mechanism for neuroprotection against mild cerebral ischemic injuries. However, NSSE was unable to prevent loss of neuronal plasticity. 2014 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/36150/1/COnference_2014.pdf Babar, Z.M. and Azzubaidi, Marwan Saad and Sulaiman , Wan Azizi and Talib, Norlelawati A. (2014) Hippocampal GFAP and MAP2 mRNA expressions of curry leaves extract treatment in 2VO rats of neurodegeneration. In: International Conference on Natural Products 2014, 18th-19th March 2014, Palm Garden Hotel, IOI Resorts, Putrajaya, Malaysia. http://www.ukm.my/icnp2014/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action
spellingShingle	RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action Babar, Z.M. Azzubaidi, Marwan Saad Sulaiman , Wan Azizi Talib, Norlelawati A. Hippocampal GFAP and MAP2 mRNA expressions of curry leaves extract treatment in 2VO rats of neurodegeneration
description	Background: Experimental neurodegeneration can be induced by two-vessel occlusion intervention (2VO). Nigella sativa (Linn.) seeds extract (NSSE) has recently shown to enhance memory and learning task performances in rats with 2VO. Prevention of neuroinflammation and/or of toxic free radical formation are possible mechanisms. The glial fibrillary acidic protein (GFAP) is a vital neuroinflammatory marker in neuroscience researches. GFAP was found to be up-regulated in 2VO rats indicating astrogliosis. Microtubule associated protein type II (MAP2) is an important marker for neuroprotection during and after ischemic brain injury or hypoxia. It was found to be down-regulated in 2VO rats. Objective: The current study aimed to compare the hippocampal MAP-2 and GFAP mRNA expressions of 2VO (untreated) male rats in the experimental neurodegeneration with other groups of rats treated with NSSE. Method: 18 adult male Sprague Dawley rats were randomly divided into 3 groups (n=6); Healthy Control (HC); 2VO- without treatment (2VO); 2VO+NSSE-treated (NSSE). The NSSE group was pre-treated (2ml, 50 mg/kg/day, orally) for 10 days prior to 2VO surgery and continued until all animals were sacrificed at the end of 10th postoperative week. Hippocampal samples were then collected; total RNA was extracted, purified and relatively quantified via reverse transcribed cDNA as per ∆∆Cq of RT-qPCR assay. Results: There was a significant 3 folds difference of GFAP mRNA expression in both HC and NSSE groups as compared to that of 2VO. However, GFAP mRNA expression difference was not significant (P=0.52) of for NSSE vs. HC. Hippocampal MAP2 mRNA expression of NSSE group appeared to be insignificantly different from the untreated 2VO group. Conclusion: Prolonged treatment with NSSE has the potential to prevent hypoperfusion-induced neuroinflammation within the hippocampus. This may be a potential mechanism for neuroprotection against mild cerebral ischemic injuries. However, NSSE was unable to prevent loss of neuronal plasticity.
format	Conference or Workshop Item
author	Babar, Z.M. Azzubaidi, Marwan Saad Sulaiman , Wan Azizi Talib, Norlelawati A.
author_facet	Babar, Z.M. Azzubaidi, Marwan Saad Sulaiman , Wan Azizi Talib, Norlelawati A.
author_sort	Babar, Z.M.
title	Hippocampal GFAP and MAP2 mRNA expressions of curry leaves extract treatment in 2VO rats of neurodegeneration
title_short	Hippocampal GFAP and MAP2 mRNA expressions of curry leaves extract treatment in 2VO rats of neurodegeneration
title_full	Hippocampal GFAP and MAP2 mRNA expressions of curry leaves extract treatment in 2VO rats of neurodegeneration
title_fullStr	Hippocampal GFAP and MAP2 mRNA expressions of curry leaves extract treatment in 2VO rats of neurodegeneration
title_full_unstemmed	Hippocampal GFAP and MAP2 mRNA expressions of curry leaves extract treatment in 2VO rats of neurodegeneration
title_sort	hippocampal gfap and map2 mrna expressions of curry leaves extract treatment in 2vo rats of neurodegeneration
publishDate	2014
url	http://irep.iium.edu.my/36150/ http://irep.iium.edu.my/36150/ http://irep.iium.edu.my/36150/1/COnference_2014.pdf
first_indexed	2018-09-07T05:45:08Z
last_indexed	2018-09-07T05:45:08Z
_version_	1610926374535561216

Hippocampal GFAP and MAP2 mRNA expressions of curry leaves extract treatment in 2VO rats of neurodegeneration

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