Celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats
Objectives: Reduced cerebral blood flow (CBF)has been associated with neurodegenerative disorders. Since neuroinflammation is thought to play a significant role in chronic degenerative neurological disorders like Alzheimer's disease, the present study was planned to,assess the neuroprotective...
| Main Authors: | , , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English English English |
| Published: |
2013
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/33808/ http://irep.iium.edu.my/33808/ http://irep.iium.edu.my/33808/1/Oral_Presentation_Certificate.pdf http://irep.iium.edu.my/33808/2/Abstract.pdf http://irep.iium.edu.my/33808/3/Cover_Page.pdf |
| id |
iium-33808 |
|---|---|
| recordtype |
eprints |
| spelling |
iium-338082014-01-07T07:30:43Z http://irep.iium.edu.my/33808/ Celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats Saxena, Anil Kumar Mohd, Fadly Talib, Norlelawati A. R Medicine (General) RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action Objectives: Reduced cerebral blood flow (CBF)has been associated with neurodegenerative disorders. Since neuroinflammation is thought to play a significant role in chronic degenerative neurological disorders like Alzheimer's disease, the present study was planned to,assess the neuroprotective role of celecoxib in Alzheimer's model of rats(2VO). Methods:Experimentally,a condition of chronic cerebral hypoperfusion due to reduced CBF can be induced by permanent bilateral occlusion of common carotid arteries (2VO)in rats. After 1 week of acclimatization,fifteen Sprague Dawley rats weighing 200-250 g were equally divided into three groups. Group A served as - sham control, Group B - 2VO, and Group C - 2VO-C treated daily with celecoxib 50 mg/kg, orally following 2VO. On 8th week, all the rats were euthanized and the hippocampi were isolated. Viable neuronal cells in the hippocampal CA-1 region were counted and hippocampal COX-2 mRNA expression and prostaglandin E2(PGE-2)levels were estimated. Results: There was a significant difference in neuronal cell death, increase in COX-2 mRNA expression and PGE-2 levels in 2VO group as compared to sham control group. In celecoxib-treated 2VO-C rats,the viable neuronal cell count of the hippocampal CA-l region was significantly higher as compared to the untreated 2VO group. The hippocampal COX-2 mRNA expression and hippocampal PGE-2 levels were found to be significantly lower in the celecoxib-treated 2VO rats as compared to untreated 2VO rats. Conclusions: The results indicate that celecoxib could be successfully used in the management of Alzheimer's disease. No conflict of interest. 2013-12-08 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/33808/1/Oral_Presentation_Certificate.pdf application/pdf en http://irep.iium.edu.my/33808/2/Abstract.pdf application/pdf en http://irep.iium.edu.my/33808/3/Cover_Page.pdf Saxena, Anil Kumar and Mohd, Fadly and Talib, Norlelawati A. (2013) Celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats. In: XX World Congress on Parkinson"s Disease and Related Disorders, 8-11 Dec 2013 , Geneva, Switzerland. http://www.kenes.com/parkinson |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English English English |
| topic |
R Medicine (General) RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action |
| spellingShingle |
R Medicine (General) RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry RM300 Drugs and their action Saxena, Anil Kumar Mohd, Fadly Talib, Norlelawati A. Celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats |
| description |
Objectives: Reduced cerebral blood flow (CBF)has been associated with neurodegenerative disorders. Since neuroinflammation is thought to play a significant role in chronic degenerative neurological disorders like Alzheimer's
disease, the present study was planned to,assess the neuroprotective role of celecoxib in Alzheimer's model of rats(2VO).
Methods:Experimentally,a condition of chronic cerebral hypoperfusion due to reduced CBF can be induced by permanent
bilateral occlusion of common carotid arteries (2VO)in rats.
After 1 week of acclimatization,fifteen Sprague Dawley rats weighing 200-250 g were equally divided into three groups. Group A served as - sham control, Group B - 2VO, and Group C - 2VO-C treated daily with celecoxib 50 mg/kg, orally following 2VO. On 8th week, all the rats were euthanized and
the hippocampi were isolated. Viable neuronal cells in the hippocampal CA-1 region were counted and hippocampal COX-2 mRNA expression and prostaglandin E2(PGE-2)levels were estimated.
Results: There was a significant difference in neuronal cell
death, increase in COX-2 mRNA expression and PGE-2 levels in
2VO group as compared to sham control group. In celecoxib-treated 2VO-C rats,the viable neuronal cell count of the
hippocampal CA-l region was significantly higher as compared
to the untreated 2VO group. The hippocampal COX-2 mRNA expression and hippocampal PGE-2 levels were found to be significantly lower in the celecoxib-treated 2VO rats as compared to untreated 2VO rats.
Conclusions: The results indicate that celecoxib could be successfully used in the management of Alzheimer's disease.
No conflict of interest. |
| format |
Conference or Workshop Item |
| author |
Saxena, Anil Kumar Mohd, Fadly Talib, Norlelawati A. |
| author_facet |
Saxena, Anil Kumar Mohd, Fadly Talib, Norlelawati A. |
| author_sort |
Saxena, Anil Kumar |
| title |
Celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats |
| title_short |
Celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats |
| title_full |
Celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats |
| title_fullStr |
Celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats |
| title_full_unstemmed |
Celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats |
| title_sort |
celecoxib as a neuroprotective agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats |
| publishDate |
2013 |
| url |
http://irep.iium.edu.my/33808/ http://irep.iium.edu.my/33808/ http://irep.iium.edu.my/33808/1/Oral_Presentation_Certificate.pdf http://irep.iium.edu.my/33808/2/Abstract.pdf http://irep.iium.edu.my/33808/3/Cover_Page.pdf |
| first_indexed |
2018-09-07T05:34:06Z |
| last_indexed |
2018-09-07T05:34:06Z |
| _version_ |
1610925680016490496 |