Participation of annexin 1 in the response of Arabidopsis thaliana to lead exposure: potential for phytoremediation

Heavy metal pollution has become a serious public health and environmental concern. Lead (Pb) is one of the heavy metals known to bioaccumulate in plants. Phytoremediation is an emerging technology based on the ability of green plants to remove Pb from the environment in a cost-efficient and ecologi...

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Main Authors:	Phang, Ing Chia, Leung, David W. M., Clark, Gregory B., Taylor, Harry H., Garrill, Ashley, Song, Jason, Roux, Stanley J.
Format:	Conference or Workshop Item
Language:	English
Published:	2009
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/309/ http://irep.iium.edu.my/309/ http://irep.iium.edu.my/309/1/Participation_of_annexin_1_in_the_response_of_Arabidopis_thaliana_sedlings.pdf

id	iium-309
recordtype	eprints
spelling	iium-3092011-11-21T12:48:03Z http://irep.iium.edu.my/309/ Participation of annexin 1 in the response of Arabidopsis thaliana to lead exposure: potential for phytoremediation Phang, Ing Chia Leung, David W. M. Clark, Gregory B. Taylor, Harry H. Garrill, Ashley Song, Jason Roux, Stanley J. Q Science (General) Heavy metal pollution has become a serious public health and environmental concern. Lead (Pb) is one of the heavy metals known to bioaccumulate in plants. Phytoremediation is an emerging technology based on the ability of green plants to remove Pb from the environment in a cost-efficient and ecologically sound manner. Currently, an important research focus is to seek a better understanding of the mechanisms of Pb tolerance by plant cells, with the aim of genetically engineering plants with improved tolerance to Pb, and hence better phytoremediation capabilities in the near future. Annexin, a calcium-dependent membrane-binding protein is believed to play a role in many essential cellular processes. It has been shown that expression of annexin genes from Arabidopsis thaliana are differentially regulated in response to a variety of abiotic stresses. Thus annexins are likely be involved in the response of plants to heavy metal stress. This study aimed to obtain new insights into whether annexin 1 (AnnAt1), is involved in Pb tolerance in plant cells. Message levels of AnnAt1 were assessed in response to Pb treatments using quantitative real-time PCR. Expression results were analysed using REST 2008 and normalized against the mitosis protein YLS8. We found that Pb effect on AnnAt1 expression in plants exposed to lower Pb concentrations (25 µM, 50 µM, and 75 µM) was not significantly different from the controls. However, AnnAt1 message levels doubled (2.12-fold, S.E. range is 1.77 - 2.61, p < 0.001) in seedlings treated with 100 µM Pb, in comparison to the control plants. The relative contribution of AnnAt1 in defence against Pb stress will be discussed. 2009-12-01 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/309/1/Participation_of_annexin_1_in_the_response_of_Arabidopis_thaliana_sedlings.pdf Phang, Ing Chia and Leung, David W. M. and Clark, Gregory B. and Taylor, Harry H. and Garrill, Ashley and Song, Jason and Roux, Stanley J. (2009) Participation of annexin 1 in the response of Arabidopsis thaliana to lead exposure: potential for phytoremediation. In: 6th International Phytotechnologies Conference, 2-4 December 2009, St. Louis Reverfront, Hyatt Regency, Missouri, USA. http://phyto.mst.edu/2009_conference/Poster_Sessions.html
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Phang, Ing Chia Leung, David W. M. Clark, Gregory B. Taylor, Harry H. Garrill, Ashley Song, Jason Roux, Stanley J. Participation of annexin 1 in the response of Arabidopsis thaliana to lead exposure: potential for phytoremediation
description	Heavy metal pollution has become a serious public health and environmental concern. Lead (Pb) is one of the heavy metals known to bioaccumulate in plants. Phytoremediation is an emerging technology based on the ability of green plants to remove Pb from the environment in a cost-efficient and ecologically sound manner. Currently, an important research focus is to seek a better understanding of the mechanisms of Pb tolerance by plant cells, with the aim of genetically engineering plants with improved tolerance to Pb, and hence better phytoremediation capabilities in the near future. Annexin, a calcium-dependent membrane-binding protein is believed to play a role in many essential cellular processes. It has been shown that expression of annexin genes from Arabidopsis thaliana are differentially regulated in response to a variety of abiotic stresses. Thus annexins are likely be involved in the response of plants to heavy metal stress. This study aimed to obtain new insights into whether annexin 1 (AnnAt1), is involved in Pb tolerance in plant cells. Message levels of AnnAt1 were assessed in response to Pb treatments using quantitative real-time PCR. Expression results were analysed using REST 2008 and normalized against the mitosis protein YLS8. We found that Pb effect on AnnAt1 expression in plants exposed to lower Pb concentrations (25 µM, 50 µM, and 75 µM) was not significantly different from the controls. However, AnnAt1 message levels doubled (2.12-fold, S.E. range is 1.77 - 2.61, p < 0.001) in seedlings treated with 100 µM Pb, in comparison to the control plants. The relative contribution of AnnAt1 in defence against Pb stress will be discussed.
format	Conference or Workshop Item
author	Phang, Ing Chia Leung, David W. M. Clark, Gregory B. Taylor, Harry H. Garrill, Ashley Song, Jason Roux, Stanley J.
author_facet	Phang, Ing Chia Leung, David W. M. Clark, Gregory B. Taylor, Harry H. Garrill, Ashley Song, Jason Roux, Stanley J.
author_sort	Phang, Ing Chia
title	Participation of annexin 1 in the response of Arabidopsis thaliana to lead exposure: potential for phytoremediation
title_short	Participation of annexin 1 in the response of Arabidopsis thaliana to lead exposure: potential for phytoremediation
title_full	Participation of annexin 1 in the response of Arabidopsis thaliana to lead exposure: potential for phytoremediation
title_fullStr	Participation of annexin 1 in the response of Arabidopsis thaliana to lead exposure: potential for phytoremediation
title_full_unstemmed	Participation of annexin 1 in the response of Arabidopsis thaliana to lead exposure: potential for phytoremediation
title_sort	participation of annexin 1 in the response of arabidopsis thaliana to lead exposure: potential for phytoremediation
publishDate	2009
url	http://irep.iium.edu.my/309/ http://irep.iium.edu.my/309/ http://irep.iium.edu.my/309/1/Participation_of_annexin_1_in_the_response_of_Arabidopis_thaliana_sedlings.pdf
first_indexed	2018-09-07T02:50:47Z
last_indexed	2018-09-07T02:50:47Z
_version_	1610915405121978368

Participation of annexin 1 in the response of Arabidopsis thaliana to lead exposure: potential for phytoremediation

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