Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor Development

In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the...

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Main Authors:	Jouko Peltonen, Ronald Österbacka, Marjo Yliperttula, Jawad Sarfraz, Harri Härmä, Anni Määttänen, Tuomas Närjeoja, Tapani Viitala, Björn Törngren, Himadri Majumdar, Petri Ihalainen
Format:	Article
Language:	English
Published:	MDPI AG 2012-12-01
Series:	Biosensors
Subjects:	paper electronics nanoparticles inkjet printing immunoassays impedance spectroscopy
Online Access:	http://www.mdpi.com/2079-6374/3/1/1

id	doaj-art-fa0d03636f7b4b73a595c4c29d7ee11f
recordtype	oai_dc
spelling	doaj-art-fa0d03636f7b4b73a595c4c29d7ee11f2018-09-02T22:44:37ZengMDPI AGBiosensors2079-63742012-12-013111710.3390/bios3010001Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor DevelopmentJouko PeltonenRonald ÖsterbackaMarjo YliperttulaJawad SarfrazHarri HärmäAnni MäättänenTuomas NärjeojaTapani ViitalaBjörn TörngrenHimadri MajumdarPetri IhalainenIn this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where—(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth—all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.http://www.mdpi.com/2079-6374/3/1/1paper electronicsnanoparticlesinkjet printingimmunoassaysimpedance spectroscopy
institution	Open Data Bank
collection	Open Access Journals
building	Directory of Open Access Journals
language	English
format	Article
author	Jouko Peltonen Ronald Österbacka Marjo Yliperttula Jawad Sarfraz Harri Härmä Anni Määttänen Tuomas Närjeoja Tapani Viitala Björn Törngren Himadri Majumdar Petri Ihalainen
spellingShingle	Jouko Peltonen Ronald Österbacka Marjo Yliperttula Jawad Sarfraz Harri Härmä Anni Määttänen Tuomas Närjeoja Tapani Viitala Björn Törngren Himadri Majumdar Petri Ihalainen Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor Development Biosensors paper electronics nanoparticles inkjet printing immunoassays impedance spectroscopy
author_facet	Jouko Peltonen Ronald Österbacka Marjo Yliperttula Jawad Sarfraz Harri Härmä Anni Määttänen Tuomas Närjeoja Tapani Viitala Björn Törngren Himadri Majumdar Petri Ihalainen
author_sort	Jouko Peltonen
title	Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor Development
title_short	Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor Development
title_full	Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor Development
title_fullStr	Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor Development
title_full_unstemmed	Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor Development
title_sort	application of paper-supported printed gold electrodes for impedimetric immunosensor development
publisher	MDPI AG
series	Biosensors
issn	2079-6374
publishDate	2012-12-01
description	In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where—(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth—all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.
topic	paper electronics nanoparticles inkjet printing immunoassays impedance spectroscopy
url	http://www.mdpi.com/2079-6374/3/1/1
_version_	1612621463991353344

Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor Development

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