Human lung and monocyte-derived macrophages differ with regard to the effects of β2-adrenoceptor agonists on cytokine release
Abstract Background β2-adrenoceptor agonists have been shown to reduce the lipopolysaccharide (LPS)-induced cytokine release by human monocyte-derived macrophages (MDMs). We compare the expression of β2-adrenoceptors and the inhibitory effect of formoterol and salmeterol on the LPS-induced release o...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BioMed Central
2017-06-01
|
| Series: | Respiratory Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12931-017-0613-y |
| id |
doaj-art-ee563f613beb4309b150535514c40dfa |
|---|---|
| recordtype |
oai_dc |
| spelling |
doaj-art-ee563f613beb4309b150535514c40dfa2018-08-15T23:45:35ZengBioMed CentralRespiratory Research1465-993X2017-06-0118111010.1186/s12931-017-0613-yHuman lung and monocyte-derived macrophages differ with regard to the effects of β2-adrenoceptor agonists on cytokine releaseTatiana Victoni0Hélène Salvator1Charlotte Abrial2Marion Brollo3Luis Cristovão Sobrino Porto4Vincent Lagente5Emmanuel Naline6Stanislas Grassin-Delyle7Philippe Devillier8Laboratory of Histocompatibility and Cryopresevation, Laboratory of Tissue RepairLaboratory of Research in Respiratory Pharmacology–UPRES EA220, UFR Sciences de la Santé Simone Veil, Université Versailles Saint-Quentin, Université Paris-SaclayLaboratory of Research in Respiratory Pharmacology–UPRES EA220, UFR Sciences de la Santé Simone Veil, Université Versailles Saint-Quentin, Université Paris-SaclayLaboratory of Research in Respiratory Pharmacology–UPRES EA220, UFR Sciences de la Santé Simone Veil, Université Versailles Saint-Quentin, Université Paris-SaclayLaboratory of Histocompatibility and Cryopresevation, Laboratory of Tissue RepairNutrition Metabolisms and Cancer, INSERM, INRA, Université Rennes 1, Université Bretagne LoireLaboratory of Research in Respiratory Pharmacology–UPRES EA220, UFR Sciences de la Santé Simone Veil, Université Versailles Saint-Quentin, Université Paris-SaclayDepartment of Airway Diseases, Foch HospitalLaboratory of Research in Respiratory Pharmacology–UPRES EA220, UFR Sciences de la Santé Simone Veil, Université Versailles Saint-Quentin, Université Paris-SaclayAbstract Background β2-adrenoceptor agonists have been shown to reduce the lipopolysaccharide (LPS)-induced cytokine release by human monocyte-derived macrophages (MDMs). We compare the expression of β2-adrenoceptors and the inhibitory effect of formoterol and salmeterol on the LPS-induced release of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and a range of chemokines (CCL2, 3, 4, and IL-8) by human lung macrophages (LMs) and MDMs. Methods LMs were isolated from patients undergoing resection and MDMs were obtained from blood monocytes in the presence of GM-CSF. LMs and MDMs were incubated in the absence or presence of formoterol or salmeterol prior to stimulation with LPS. The effects of formoterol were also assessed in the presence of the phosphodiesterase inhibitor roflumilast. Results LPS-induced cytokine production was higher in LMs than in MDMs. Salmeterol and formoterol exerted an inhibitory effect on the LPS-induced production of TNF-α, IL-6, CCL2, CCL3, and CCL4 in MDMs. In contrast, the β2-adrenoceptor agonists were devoid of any effect on LMs - even in the presence of roflumilast. The expression of β2-adrenergic receptors was detected on Western blots in MDMs but not in LMs. Conclusions Concentrations of β2-adrenoceptor agonists that cause relaxation of the human bronchus can inhibit cytokine production by LPS-stimulated MDMs but not by LMs.http://link.springer.com/article/10.1186/s12931-017-0613-yβ2-adrenoceptorCytokinesLipopolysaccharideLung macrophageMonocyte-derived macrophage |
| institution |
Open Data Bank |
| collection |
Open Access Journals |
| building |
Directory of Open Access Journals |
| language |
English |
| format |
Article |
| author |
Tatiana Victoni Hélène Salvator Charlotte Abrial Marion Brollo Luis Cristovão Sobrino Porto Vincent Lagente Emmanuel Naline Stanislas Grassin-Delyle Philippe Devillier |
| spellingShingle |
Tatiana Victoni Hélène Salvator Charlotte Abrial Marion Brollo Luis Cristovão Sobrino Porto Vincent Lagente Emmanuel Naline Stanislas Grassin-Delyle Philippe Devillier Human lung and monocyte-derived macrophages differ with regard to the effects of β2-adrenoceptor agonists on cytokine release Respiratory Research β2-adrenoceptor Cytokines Lipopolysaccharide Lung macrophage Monocyte-derived macrophage |
| author_facet |
Tatiana Victoni Hélène Salvator Charlotte Abrial Marion Brollo Luis Cristovão Sobrino Porto Vincent Lagente Emmanuel Naline Stanislas Grassin-Delyle Philippe Devillier |
| author_sort |
Tatiana Victoni |
| title |
Human lung and monocyte-derived macrophages differ with regard to the effects of β2-adrenoceptor agonists on cytokine release |
| title_short |
Human lung and monocyte-derived macrophages differ with regard to the effects of β2-adrenoceptor agonists on cytokine release |
| title_full |
Human lung and monocyte-derived macrophages differ with regard to the effects of β2-adrenoceptor agonists on cytokine release |
| title_fullStr |
Human lung and monocyte-derived macrophages differ with regard to the effects of β2-adrenoceptor agonists on cytokine release |
| title_full_unstemmed |
Human lung and monocyte-derived macrophages differ with regard to the effects of β2-adrenoceptor agonists on cytokine release |
| title_sort |
human lung and monocyte-derived macrophages differ with regard to the effects of β2-adrenoceptor agonists on cytokine release |
| publisher |
BioMed Central |
| series |
Respiratory Research |
| issn |
1465-993X |
| publishDate |
2017-06-01 |
| description |
Abstract Background β2-adrenoceptor agonists have been shown to reduce the lipopolysaccharide (LPS)-induced cytokine release by human monocyte-derived macrophages (MDMs). We compare the expression of β2-adrenoceptors and the inhibitory effect of formoterol and salmeterol on the LPS-induced release of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6 and a range of chemokines (CCL2, 3, 4, and IL-8) by human lung macrophages (LMs) and MDMs. Methods LMs were isolated from patients undergoing resection and MDMs were obtained from blood monocytes in the presence of GM-CSF. LMs and MDMs were incubated in the absence or presence of formoterol or salmeterol prior to stimulation with LPS. The effects of formoterol were also assessed in the presence of the phosphodiesterase inhibitor roflumilast. Results LPS-induced cytokine production was higher in LMs than in MDMs. Salmeterol and formoterol exerted an inhibitory effect on the LPS-induced production of TNF-α, IL-6, CCL2, CCL3, and CCL4 in MDMs. In contrast, the β2-adrenoceptor agonists were devoid of any effect on LMs - even in the presence of roflumilast. The expression of β2-adrenergic receptors was detected on Western blots in MDMs but not in LMs. Conclusions Concentrations of β2-adrenoceptor agonists that cause relaxation of the human bronchus can inhibit cytokine production by LPS-stimulated MDMs but not by LMs. |
| topic |
β2-adrenoceptor Cytokines Lipopolysaccharide Lung macrophage Monocyte-derived macrophage |
| url |
http://link.springer.com/article/10.1186/s12931-017-0613-y |
| _version_ |
1612699427620782080 |