Local sequence and sequencing depth dependent accuracy of RNA-seq reads

Local sequence and sequencing depth dependent accuracy of RNA-seq reads

Abstract Background Many biases and spurious effects are inherent in RNA-seq technology, resulting in a non-uniform distribution of sequencing read counts for each base position in a gene. Therefore, a base-level strategy is required to model the non-uniformity. Also, the properties of sequencing re...

Full description

Bibliographic Details
Main Authors: Guoshuai Cai, Shoudan Liang, Xiaofeng Zheng, Feifei Xiao
Format: Article
Language:English
Published: BioMed Central 2017-08-01
Series:BMC Bioinformatics
Subjects:
RNA-seq
Non-uniformity
Bias
Base-level modeling
Overdispersion
Beta-binomial
Online Access:http://link.springer.com/article/10.1186/s12859-017-1780-z

Internet

http://link.springer.com/article/10.1186/s12859-017-1780-z

Similar Items