PHYSICAL AND IMMUNOBIOLOGICAL STUDIES OF TEICHOIC ACIDS OF PROBIOTIC LACTOBACILLI STRAINS

Aim. The aim of this study was to establish the chemical structure of teichoic acids of probiotic lactobacilli strains and to investigate their influence on murine macrophages phagocytosis and proliferation of the murine macrophages cell line J 774. Methods. Physical (infrared spectroscopy methodolo...

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Main Authors:	О. П. Лівінська, O. M. Василенко, T. В. Томила, I. Л. Гармашева, Н. К. Коваленко
Format:	Article
Language:	English
Published:	Odessa I. I. Mechnikov National University 2015-08-01
Series:	Mìkrobìologìâ ì Bìotehnologìâ
Subjects:	тейхоєві кислоти ЯМР спектроскопія інфрачервона спектроскопія фагоцитоз пробіотики
Online Access:	http://mbt.onu.edu.ua/article/view/48394

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spelling	doaj-art-c458f6b886ad463da144f1a532cce2e02018-09-04T06:12:10ZengOdessa I. I. Mechnikov National University Mìkrobìologìâ ì Bìotehnologìâ2076-05582307-46632015-08-0104(28)61410.18524/2307-4663.2014.4(28).4839448394PHYSICAL AND IMMUNOBIOLOGICAL STUDIES OF TEICHOIC ACIDS OF PROBIOTIC LACTOBACILLI STRAINSО. П. Лівінська0O. M. Василенко1T. В. Томила2I. Л. Гармашева3Н. К. Коваленко4Інститут мікробіології і вірусології ім. Д.К. Заболотного НАНУІнститут біоорганічної хімії та нафтохімії НАНУІнститут проблем матеріалознавства ім. І.М. Францевича НАНУІнститут мікробіології і вірусології ім. Д.К. Заболотного НАНУІнститут мікробіології і вірусології ім. Д.К. Заболотного НАНУAim. The aim of this study was to establish the chemical structure of teichoic acids of probiotic lactobacilli strains and to investigate their influence on murine macrophages phagocytosis and proliferation of the murine macrophages cell line J 774. Methods. Physical (infrared spectroscopy methodology, 1H, and 13C NMR experiments), immunobiological and statistical methods were used. Results. It was shown that cell walls of Lactobacillus plantarum 11/16 and Lactobacillus plantarum 195D contained glycerol teichoic acids with unusually high number of sugar elements. The teichoic acid of the strain L. plantarum 11/16 had 9 different pyranoses and furanoses sugars in alfa-configuration and L. plantarum 195D had 4 hexosopyranoses. The teichoic acids of L. plantarum 195D and 11/16 increased the percent phagocytosis by 15.00±5.72% and 13.75±4.65% respectively. Both teichoic acids in the doses of 32 and 64 μg/ml stimulated cell proliferation of murine macrophage cell line by 35–53%. It was not observed any considerable effect of studied teichoic acids on phagocytic index, pontaneous and stimulated NBT-test of peritoneal macrophages of mice.http://mbt.onu.edu.ua/article/view/48394тейхоєві кислотиЯМР спектроскопіяінфрачервона спектроскопіяфагоцитозпробіотики
institution	Open Data Bank
collection	Open Access Journals
building	Directory of Open Access Journals
language	English
format	Article
author	О. П. Лівінська O. M. Василенко T. В. Томила I. Л. Гармашева Н. К. Коваленко
spellingShingle	О. П. Лівінська O. M. Василенко T. В. Томила I. Л. Гармашева Н. К. Коваленко PHYSICAL AND IMMUNOBIOLOGICAL STUDIES OF TEICHOIC ACIDS OF PROBIOTIC LACTOBACILLI STRAINS Mìkrobìologìâ ì Bìotehnologìâ тейхоєві кислоти ЯМР спектроскопія інфрачервона спектроскопія фагоцитоз пробіотики
author_facet	О. П. Лівінська O. M. Василенко T. В. Томила I. Л. Гармашева Н. К. Коваленко
author_sort	О. П. Лівінська
title	PHYSICAL AND IMMUNOBIOLOGICAL STUDIES OF TEICHOIC ACIDS OF PROBIOTIC LACTOBACILLI STRAINS
title_short	PHYSICAL AND IMMUNOBIOLOGICAL STUDIES OF TEICHOIC ACIDS OF PROBIOTIC LACTOBACILLI STRAINS
title_full	PHYSICAL AND IMMUNOBIOLOGICAL STUDIES OF TEICHOIC ACIDS OF PROBIOTIC LACTOBACILLI STRAINS
title_fullStr	PHYSICAL AND IMMUNOBIOLOGICAL STUDIES OF TEICHOIC ACIDS OF PROBIOTIC LACTOBACILLI STRAINS
title_full_unstemmed	PHYSICAL AND IMMUNOBIOLOGICAL STUDIES OF TEICHOIC ACIDS OF PROBIOTIC LACTOBACILLI STRAINS
title_sort	physical and immunobiological studies of teichoic acids of probiotic lactobacilli strains
publisher	Odessa I. I. Mechnikov National University
series	Mìkrobìologìâ ì Bìotehnologìâ
issn	2076-0558 2307-4663
publishDate	2015-08-01
description	Aim. The aim of this study was to establish the chemical structure of teichoic acids of probiotic lactobacilli strains and to investigate their influence on murine macrophages phagocytosis and proliferation of the murine macrophages cell line J 774. Methods. Physical (infrared spectroscopy methodology, 1H, and 13C NMR experiments), immunobiological and statistical methods were used. Results. It was shown that cell walls of Lactobacillus plantarum 11/16 and Lactobacillus plantarum 195D contained glycerol teichoic acids with unusually high number of sugar elements. The teichoic acid of the strain L. plantarum 11/16 had 9 different pyranoses and furanoses sugars in alfa-configuration and L. plantarum 195D had 4 hexosopyranoses. The teichoic acids of L. plantarum 195D and 11/16 increased the percent phagocytosis by 15.00±5.72% and 13.75±4.65% respectively. Both teichoic acids in the doses of 32 and 64 μg/ml stimulated cell proliferation of murine macrophage cell line by 35–53%. It was not observed any considerable effect of studied teichoic acids on phagocytic index, pontaneous and stimulated NBT-test of peritoneal macrophages of mice.
topic	тейхоєві кислоти ЯМР спектроскопія інфрачервона спектроскопія фагоцитоз пробіотики
url	http://mbt.onu.edu.ua/article/view/48394
_version_	1612619245583073280

PHYSICAL AND IMMUNOBIOLOGICAL STUDIES OF TEICHOIC ACIDS OF PROBIOTIC LACTOBACILLI STRAINS

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